Tgtp1 (NM_011579) Mouse Untagged Clone

CAT#: MC208047

Tgtp1 (untagged) - Mouse T-cell specific GTPase 1 (Tgtp1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tgtp1
• Synonyms: Gtp2; Irgb6; Mg21; Tgtp
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC208047 representing NM_011579
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTGGGCCTCCAGCTTTGATGCATTCTTTAAGAATTTTAAAAGGGAAAGCAAAATCATCTCTGAAT
  ATGACATCACCTTGATTATGACTTACATAGAGGAAAATAAGCTACAGAAAGCTGTTTCTGTAATTGAAAA
  GGTACTGAGAGACATCGAGAGTGCTCCTCTGCACATAGCTGTGACAGGGGAAACAGGCGCAGGGAAGTCC
  ACTTTCATCAATACCCTGAGGGGGGTGGGGCATGAAGAAAAAGGTGCAGCCCCCACTGGGGCAATAGAGA
  CAACCATGAAGAGAACTCCATACCCACACCCAAAGCTTCCCAACGTGACAATATGGGACCTGCCTGGCAT
  TGGGACCACTAACTTCACACCACAAAACTATCTGACAGAAATGAAGTTTGGTGAGTACGACTTCTTCATT
  ATCATCTCAGCTACACGTTTCAAAGAAAATGATGCACAACTGGCCAAAGCCATTGCACAGATGGGGATGA
  ATTTCTACTTTGTCAGAACCAAGATAGACAGCGACTTAGATAATGAACAGAAGTTTAAGCCTAAGAGTTT
  CAATAAGGAGGAAGTCCTCAAGAATATTAAGGATTACTGCTCTAATCATCTTCAGGAGTCTCTCGACAGT
  GAGCCTCCAGTCTTCCTAGTCTCTAACGTTGATATATCGAAGTATGACTTCCCAAAGCTGGAAACTAAAC
  TCCTACAGGATCTCCCAGCCCACAAGCGCCACGTCTTCTCACTGTCTTTGCAAAGTCTTACTGAGGCCAC
  CATTAACTACAAGAGAGATTCCCTGAAGCAAAAAGTCTTCCTAGAAGCCATGAAGGCTGGAGCATTAGCC
  ACCATTCCACTTGGTGGCATGATCAGTGATATCTTAGAGAATCTGGATGAAACATTCAATCTCTACAGGT
  CTTACTTTGGGCTGGATGATGCTTCACTGGAAAACATTGCCCAGGATTTGAACATGTCTGTGGATGACTT
  CAAGGTACACCTTCGATTTCCCCATTTGTTTGCAGAACACAATGATGAGTCCTTAGAAGACAAGCTATTT
  AAATATATCAAACACATTTCTTCAGTTACTGGTGGGCCAGTCGCTGCAGTCACTTACTATCGCATGGCTT
  ATTATTTGCAGAATCTCTTTCTTGATACTGCAGCCAATGATGCCATAGCTCTTCTGAATAGTAAAGCACT
  TTTTGAGAAGAAGGTGGGACCATATATATCTGAGCCCCCCGAGTACTGGGAAGCTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_011579
• Insert Size: 1248 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011579.3, NP_035709.3
• RefSeq Size: 2809 bp
• RefSeq ORF: 1248 bp
• Locus ID: 21822
• UniProt ID: Q62293
• Cytogenetics: 11 B1.2
• Gene Summary: Involved in innate cell-autonomous resistance to intracellular pathogens, such as Toxoplasma gondii. During avirulent type II T. gondii infection, recruited to the parasitophorous vacuole (PV) membrane, leading to PV vesiculation and rupture, and subsequent digestion of the parasite within the cytosol (PubMed:19265156, PubMed:24563254). Not recruited to virulent type I T. gondii PV membrane (PubMed:19265156). May confer an antiviral state for vesicular stomatitis virus (PubMed:9725230).[UniProtKB/Swiss-Prot Function]