Txnip (BC011212) Mouse Tagged ORF Clone

CAT#: MG206284

Txnip (tGFP-tagged) - Mouse thioredoxin interacting protein (cDNA clone MGC:18703 IMAGE:4193517)

Product Images

Circular map for MG206284

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Txnip
• Synonyms: THIF, VDUP1, mVDUP1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG206284 representing BC011212
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGATGTTCAAGAAGATCAAGTCTTTTGAGGTGGTCTTCAACGACCCCGAGAAAGTGTACGGCAGCG
  GGGAGAAGGTGGCCGGACGGGTAATTGTGGAAGTGTGTGAAGTTACCCGAGTCAAAGCTGTCAGGATCCT
  GGCTTGCGGCGTGGCCAAGGTCCTGTGGATGCAAGGGTCTCAGCAGTGCAAACAGACTTTGGACTACTTG
  CGCTATGAAGACACACTTCTCCTAGAAGAGCAGCCTACAGCAGGTGAGAACGAGATGGTGATCATGAGGC
  CTGGAAACAAATATGAGTACAAGTTCGGCTTCGAGCTTCCTAGAGGGCCCCTGGGAACATCCTTTAAAGG
  AAAATATGGTTGCGTAGACTACTGGGTGAAGGCTTTTCTCGATCGCCCCAGCCAGCCAACTCAAGAGGCA
  AAGAAAAACTTCGAAGTGATGGATCTAGTGGATGTCAATACCCCTGACTTAATGGCACCAGTGTCTGCCA
  AAAAGGAGAAGAAAGTTTCCTGCATGTTCATTCCTGATGGACGTGTGTCAGTCTCTGCTCGAATTGACCG
  AAAAGGATTCTGTGAAGGTGATGACATCTCCATCCATGCTGACTTTGAGAACACGTGTTCCCGAATCGTG
  GTCCCCAAAGCGGCTATTGTGGCCCGACACACTTACCTTGCCAATGGCCAGACCAAAGTGTTCACTCAGA
  AGCTGTCCTCGGTCAGAGGCAATCACATTATCTCAGGGACTTGCGCATCGTGGCGTGGCAAGAGCCTCAG
  AGTGCAGAAGATCAGACCATCCATCCTGGGCTGCAACATCCTCAAAGTCGAATACTCCTTGCTGATCTAC
  GTCAGTGTCCCTGGCTCCAAGAAAGTCATCCTTGATCTGCCCCTAGTGATTGGCAGCAGGTCTGGTCTGA
  GCAGCCGGACATCCAGCATGGCCAGCCGGACGAGCTCTGAGATGAGCTGGATAGACCTAAACATCCCAGA
  TACCCCAGAAGCTCCTCCTTGCTATATGGACATCATTCCTGAAGATCACAGACTAGAGAGCCCCACCACC
  CCTCTGCTGGACGATGTGGACGACTCTCAAGACAGCCCTATCTTTATGTACGCCCCTGAGTTCCAGTTCA
  TGCCCCCACCCACTTACACTGAGGTGGATCCGTGCGTCCTTAACAACAACAACAACAACAACAACAACAG
  CAACGTGCAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG206284 representing BC011212
  Red=Cloning site Green=Tags(s)
  
  MVMFKKIKSFEVVFNDPEKVYGSGEKVAGRVIVEVCEVTRVKAVRILACGVAKVLWMQGSQQCKQTLDYL
  RYEDTLLLEEQPTAGENEMVIMRPGNKYEYKFGFELPRGPLGTSFKGKYGCVDYWVKAFLDRPSQPTQEA
  KKNFEVMDLVDVNTPDLMAPVSAKKEKKVSCMFIPDGRVSVSARIDRKGFCEGDDISIHADFENTCSRIV
  VPKAAIVARHTYLANGQTKVFTQKLSSVRGNHIISGTCASWRGKSLRVQKIRPSILGCNILKVEYSLLIY
  VSVPGSKKVILDLPLVIGSRSGLSSRTSSMASRTSSEMSWIDLNIPDTPEAPPCYMDIIPEDHRLESPTT
  PLLDDVDDSQDSPIFMYAPEFQFMPPPTYTEVDPCVLNNNNNNNNNSNVQ
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC011212
• ORF Size: 1202 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC011212, AAH11212
• RefSeq Size: 2799 bp
• RefSeq ORF: 1202 bp
• Locus ID: 56338
• Cytogenetics: 3 41.93 cM
• Gene Summary: May act as an oxidative stress mediator by inhibiting thioredoxin activity or by limiting its bioavailability. Interacts with COPS5 and restores COPS5-induced suppression of CDKN1B stability, blocking the COPS5-mediated translocation of CDKN1B from the nucleus to the cytoplasm. Inhibits the proteasomal degradation of DDIT4, and thereby contributes to the inhibition of the mammalian target of rapamycin complex 1 (mTORC1) (By similarity). Functions as a transcriptional repressor, possibly by acting as a bridge molecule between transcription factors and corepressor complexes, and over-expression will induce G0/G1 cell cycle arrest. Required for the maturation of natural killer cells. Acts as a suppressor of tumor cell growth.[UniProtKB/Swiss-Prot Function]