Dnajb12 (NM_019965) Mouse Tagged ORF Clone

CAT#: MG205857

Dnajb12 (tGFP-tagged) - Mouse DnaJ (Hsp40) homolog, subfamily B, member 12 (Dnajb12)

Product Images

Circular map for MG205857

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Dnajb12
• Synonyms: Dj10; mDj10
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG205857 representing NM_019965
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAATCCAACAAGGATGAAGCCGAGCGCTGTATCAGCATCGCCCTCAAGGCCATCCAAAGCAACCAGC
  CCGAGCGGGCGCTGCGCTTCCTGGAGAAGGCGCAGCGGCTCTACCCGACGCCACGAGTCAGCGCACTGAT
  CGAGTCCCTCAACCAAAAACCACAGTCCACCGGTGATCACCCTCAACCCACAGACACTACCCATACCACC
  ACCAAGAAGGCAGGCGGGACCGAAACCCCCTCAGCCAACGGCGAGGCAGGAGGAGGGGAGAGCGCCAAGG
  GCTATACCTCAGAGCAGGTGGCAGCTGTGAAAAGGGTCAAGCAGTGTAAAGATTACTATGAGATCCTTGG
  GGTAAGCAGAAGTGCCTCAGATGAGGACCTAAAGAAGGCCTACCGCAAGCTGGCCCTCAAGTTCCATCCA
  GACAAGAACCATGCGCCTGGGGCCACTGAAGCTTTCAAAGCCATTGGCACAGCGTATGCCGTACTCAGCA
  ACCCAGAGAAAAGGAAACAGTACGACCAGTTTGGTGATGACAAGAGCCAGGCTGCCCGGCACGGCCACAG
  CCACGGGGACTTCCACCGAGGCTTTGAGGCTGACATCTCCCCAGAAGACCTCTTTAACATGTTCTTTGGT
  GGCGGCTTTCCTTCCAGTAACGTTCATGTCTACAGCAATGGCCGAATGCGGTACACCTACCAGCAGAGGC
  AAGACCGCAGAGACAACCAGGGTGATGGCGGGCTAGGAGTGTTCGTGCAGCTGATGCCCATCCTCATCCT
  CATCCTCGTGTCTGCGCTCAGCCAGCTCATGGTGTCCAGTCCTCCCTACAGCCTGAGCCCGAGACCGTCA
  GTGGGCCACATCCACAAGCGAGTCACTGACCACCTGAACGTCGCCTACTACGTGGCAGACACCTTCTCCG
  AAGAGTACACGGGCTCCAGCCTCAAAACAGTGGAACGGAATGTAGAGGATGACTACATCGCCAACCTGCG
  CAACAACTGCTGGAAGGAGAAGCAGCAGAAGGAAGGCTTGCTGTACCGAGCCCGCTACTTTGGTGACACA
  GACATGTACCACAGAGCACAGAAGATGGGCACCCCGAGTTGCAATCGGCTGTCAGAGGTGCAGGCCTCCC
  TGCATGGA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG205857 representing NM_019965
  Red=Cloning site Green=Tags(s)
  
  MESNKDEAERCISIALKAIQSNQPERALRFLEKAQRLYPTPRVSALIESLNQKPQSTGDHPQPTDTTHTT
  TKKAGGTETPSANGEAGGGESAKGYTSEQVAAVKRVKQCKDYYEILGVSRSASDEDLKKAYRKLALKFHP
  DKNHAPGATEAFKAIGTAYAVLSNPEKRKQYDQFGDDKSQAARHGHSHGDFHRGFEADISPEDLFNMFFG
  GGFPSSNVHVYSNGRMRYTYQQRQDRRDNQGDGGLGVFVQLMPILILILVSALSQLMVSSPPYSLSPRPS
  VGHIHKRVTDHLNVAYYVADTFSEEYTGSSLKTVERNVEDDYIANLRNNCWKEKQQKEGLLYRARYFGDT
  DMYHRAQKMGTPSCNRLSEVQASLHG
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019965
• ORF Size: 1128 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019965.3
• RefSeq Size: 1789 bp
• RefSeq ORF: 1131 bp
• Locus ID: 56709
• UniProt ID: Q9QYI4
• Cytogenetics: 10 B4
• Gene Summary: Acts as a co-chaperone with HSPA8/Hsc70; required to promote protein folding and trafficking, prevent aggregation of client proteins, and promote unfolded proteins to endoplasmic reticulum-associated degradation (ERAD) pathway. Acts by determining HSPA8/Hsc70's ATPase and polypeptide-binding activities. Can also act independently of HSPA8/Hsc70: together with DNAJB14, acts as a chaperone that promotes maturation of potassium channels KCND2 and KCNH2 by stabilizing nascent channel subunits and assembling them into tetramers. While stabilization of nascent channel proteins is dependent on HSPA8/Hsc70, the process of oligomerization of channel subunits is independent of HSPA8/Hsc70. When overexpressed, forms membranous structures together with DNAJB14 and HSPA8/Hsc70 within the nucleus; the role of these structures, named DJANGOs, is still unclear.[UniProtKB/Swiss-Prot Function]