Dnajb12 (NM_019965) Mouse Untagged Clone

CAT#: MC201823

Dnajb12 (untagged) - Mouse DnaJ (Hsp40) homolog, subfamily B, member 12 (Dnajb12), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Dnajb12
• Synonyms: Dj10; mDj10
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC034162 sequence for NM_019965
  CCGGCCTGCCCCGCGACGCGCCGGCGGCTGGCGCAGCCCTTCGCTCGCCCGGCCTCCCCCTCCCTGGTTC CGTGCTCGGGTTCCGCCATGGAATCCAACAAGGATGAAGCCGAGCGCTGTATCAGCATCGCCCTCAAGGC CATCCAAAGCAACCAGCCCGAGCGGGCGCTGCGCTTCCTGGAGAAGGCGCAGCGGCTCTACCCGACGCCA CGAGTCAGCGCACTGATCGAGTCCCTCAACCAAAAACCACAGTCCACCGGTGATCACCCTCAACCCACAG ACACTACCCATACCACCACCAAGAAGGCAGGCGGGACCGAAACCCCCTCAGCCAACGGCGAGGCAGGAGG AGGGGAGAGCGCCAAGGGCTATACCTCAGAGCAGGTGGCAGCTGTGAAAAGGGTCAAGCAGTGTAAAGAT TACTATGAGATCCTTGGGGTAAGCAGAAGTGCCTCAGATGAGGACCTAAAGAAGGCCTACCGCAAGCTGG CCCTCAAGTTCCATCCAGACAAGAACCATGCGCCTGGGGCCACTGAAGCTTTCAAAGCCATTGGCACAGC GTATGCCGTACTCAGCAACCCAGAGAAAAGGAAACAGTACGACCAGTTTGGTGATGACAAGAGCCAGGCT GCCCGGCACGGCCACAGCCACGGGGACTTCCACCGAGGCTTTGAGGCTGACATCTCCCCAGAAGACCTCT TTAACATGTTCTTTGGTGGCGGCTTTCCTTCCAGTAACGTTCATGTCTACAGCAATGGCCGAATGCGGTA CACCTACCAGCAGAGGCAAGACCGCAGAGACAACCAGGGTGATGGCGGGCTAGGAGTGTTCGTGCAGCTG ATGCCCATCCTCATCCTCATCCTCGTGTCTGCGCTCAGCCAGCTCATGGTGTCCAGTCCTCCCTACAGCC TGAGCCCGAGACCGTCAGTGGGCCACATCCACAAGCGAGTCACTGACCACCTGAACGTCGCCTACTACGT GGCAGACACCTTCTCCGAAGAGTACACGGGCTCCAGCCTCAAAACAGTGGAACGGAATGTAGAGGATGAC TACATCGCCAACCTGCGCAACAACTGCTGGAAGGAGAAGCAGCAGAAGGAAGGCTTGCTGTACCGAGCCC GCTACTTTGGTGACACAGACATGTACCACAGAGCACAGAAGATGGGCACCCCGAGTTGCAATCGGCTGTC AGAGGTGCAGGCCTCCCTGCATGGATAGTTCTGGGCCAGCCACACCACCCAGGTCCAAACTATGAAATCC CTGGAGAATTTTTGGTGACACACACTGAGCCAGCCACAGTGATGGACTGTATATTTAAGGAAAGACATAC ACAGAAAAAATTTAAATGGAACTGGAAGCCAAACACCGCACAGCCATCCTCTCTCAGACAGTGAATGCAG AAAGCTCTTAGGACAGACAGACAGACAGCCTACCTCGGGCTGCTTCCTCCTCTCCCAGGGCAGGCAAGAA GCTCCGCATCACTGGCTCCTAGGATACCGAAACCATGGCAACGAAAGTAGAATGTAAAACTTGCAGCAAA TGTCTGTGGGGAAGGACTGGGGGAGGGGACACCCCACTGTGTCTCCTCCCTCCTCCCTGAGCTGCCACCA GTCACTCAAGGAGAAATCAGGCTGAGGCATGGGCCAAGGAACAGAGAGGAGAGAATTCCAGAGAACGTAA CTTAGAAATGCGTATGTATGTATATATATATCTATTTATATGTAAATAGACATATATAAAGATATATATA TATATATAAAGTCTTTTAAAACTAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_019965
• Insert Size: 1131 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC034162, AAH34162
• RefSeq Size: 1789 bp
• RefSeq ORF: 1131 bp
• Locus ID: 56709
• UniProt ID: Q9QYI4
• Cytogenetics: 10 B4
• Gene Summary: Acts as a co-chaperone with HSPA8/Hsc70; required to promote protein folding and trafficking, prevent aggregation of client proteins, and promote unfolded proteins to endoplasmic reticulum-associated degradation (ERAD) pathway. Acts by determining HSPA8/Hsc70's ATPase and polypeptide-binding activities. Can also act independently of HSPA8/Hsc70: together with DNAJB14, acts as a chaperone that promotes maturation of potassium channels KCND2 and KCNH2 by stabilizing nascent channel subunits and assembling them into tetramers. While stabilization of nascent channel proteins is dependent on HSPA8/Hsc70, the process of oligomerization of channel subunits is independent of HSPA8/Hsc70. When overexpressed, forms membranous structures together with DNAJB14 and HSPA8/Hsc70 within the nucleus; the role of these structures, named DJANGOs, is still unclear.[UniProtKB/Swiss-Prot Function]