Rell2 (NM_153793) Mouse Tagged ORF Clone

CAT#: MG204053

Rell2 (tGFP-tagged) - Mouse RIKEN cDNA 4631403P03 gene (4631403P03Rik)

Product Images

Circular map for MG204053

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Rell2
• Synonyms: 4631403P03Rik; ependolin
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG204053 representing NM_153793
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGGAACCACAGCCTGACCTGGAGCCGCCCCAACATGGGCTGTACATGCTCTTCCTGCTTGTGCTGG
  TCTTCTTCCTCATGGGCCTTGTAGGCTTCATGATCTGCCACGTGCTCAAGAAGAAAGGCTACCGCTGCCG
  CACGTCTAGGGGCTCAGAGCCTGATGATGCCCAGCTCCAGCCCCCTGAAGACGATGACGTGAATGAGGAC
  ACAGTAGAGCGGATTGTTCGCTGCATCATCCAAAATGAAGCCAATGCTGAGGCCTTGAAGGAGATGCTAG
  GGGACAGTGAAGGAGAAGGGACGGTGCAGCTCTCCAGTGTGGATGCCACCTCCAGCCTGCAGGACGGAGC
  CCCCTCCCATCATCACACAGTGCACCTTGGCTCTGCAGCTCCTTGCATCCACTGCAGCCGCAGCAAGAGG
  CCCCCACTTGTCCGTCAGGGTCGCTCTAAGGAAGGAAAAAGCCGTCCCAGACCTGGAGAGACCACTGTTT
  TTTCAGTGGGCAGATTCCGAGTAACACACATTGAGAAGCGCTACGGCCTACATGAACATCGTGATGGTTC
  CCCTACGGACAGAAGCTGGGGCTCTGGTGGAGGGCAGGAGCCAGGGGGTAGTCAGGCGGCTGGAGGAGGG
  CAGCCTAGGACAGGGACAGCTGCCATTGAGAGGCTGCTCCCTGAGCCGCCACCCTCCCAGGCCGCAGCCA
  CCCACTCAGTGCAGAACGGAAGACTCCAGGATGCCAGCCTCGTCCCTTGTACACTTGAGGGGACCCCTGG
  AACCTCTGCAGAACTGAACTTGGGCCCTAGAGGGAGAGACCCAAGCCCAGGGCTGTCTAGTCAAGAAGCA
  AATGGACAGCCAACCAAACTGGACACCTCAGGTCAGCAGGTA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG204053 representing NM_153793
  Red=Cloning site Green=Tags(s)
  
  MSEPQPDLEPPQHGLYMLFLLVLVFFLMGLVGFMICHVLKKKGYRCRTSRGSEPDDAQLQPPEDDDVNED
  TVERIVRCIIQNEANAEALKEMLGDSEGEGTVQLSSVDATSSLQDGAPSHHHTVHLGSAAPCIHCSRSKR
  PPLVRQGRSKEGKSRPRPGETTVFSVGRFRVTHIEKRYGLHEHRDGSPTDRSWGSGGGQEPGGSQAAGGG
  QPRTGTAAIERLLPEPPPSQAAATHSVQNGRLQDASLVPCTLEGTPGTSAELNLGPRGRDPSPGLSSQEA
  NGQPTKLDTSGQQV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_153793
• ORF Size: 882 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153793.1, NP_722488.1
• RefSeq Size: 2090 bp
• RefSeq ORF: 912 bp
• Locus ID: 225392
• UniProt ID: Q8BRJ3
• Cytogenetics: 18 B3
• Gene Summary: Induces activation of MAPK14/p38 cascade, when overexpressed. Induces apoptosis, when overexpressed.[UniProtKB/Swiss-Prot Function]