Gpm6a (BC023461) Mouse Tagged ORF Clone

CAT#: MG203545

Gpm6a (tGFP-tagged) - Mouse glycoprotein m6a (cDNA clone MGC:32434 IMAGE:5041793)

Product Images

Circular map for MG203545

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Gpm6a
• Synonyms: MGC38999, M6A
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG203545 representing BC023461
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGTGCTTCGAGTGCTGCATTAAATGCCTGGGAGGTATTCCCTATGCTTCTCTGATTGCAACCATCC
  TGCTGTATGCAGGCGTTGCCCTGTTCTGTGGCTGTGGCCATGAAGCCCTTTCTGGAACAGTCAACATTCT
  GCAGACCTACTTTGAGTTGGCAAGGACTGCTGGAGACACACTGGATGTTTTCACTATGATTGACATCTTT
  AAGTATGTGATCTATGGCATTGCGGCTGCTTTCTTTGTCTATGGCATTTTACTGATGGTAGAAGGTTTCT
  TCACAACTGGGGCTATCAAAGATCTCTATGGAGACTTCAAAATCACCACCTGTGGCAGATGTGTGAGCGC
  TTGGTTTATCATGCTGACATACCTCTTCATGTTGGCCTGGCTGGGAGTCACAGCTTTCACCTCACTGCCC
  GTGTACATGTATTTCAATGTGTGGACCATCTGCCGGAACACCACTCTAGTGGAGGGAGCAAATCTCTGCT
  TGGATCTGCGTCAGTTTGGGATTGTGACAATTGGAGAGGAAAAGAAAATTTGTACTGCCTCTGAGAACTT
  CCTGAGGATGTGTGAATCTACTGAGCTGAATATGACCTTCCACTTGTTCATTGTGGCACTTGCTGGAGCT
  GGAGCAGCAGTTATTGCTATGGTCCACTACCTGATGGTTCTGTCTGCCAACTGGGCCTATGTGAAAGATG
  CCTGCCGCATGCAGAAGTACGAAGACATCAAGTCAAAGGAAGAGCAGGAGCTGCACGACATCCATTCTAC
  TCGCTCTAAAGAGCGGCTCAATGCGTACACA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG203545 representing BC023461
  Red=Cloning site Green=Tags(s)
  
  MGCFECCIKCLGGIPYASLIATILLYAGVALFCGCGHEALSGTVNILQTYFELARTAGDTLDVFTMIDIF
  KYVIYGIAAAFFVYGILLMVEGFFTTGAIKDLYGDFKITTCGRCVSAWFIMLTYLFMLAWLGVTAFTSLP
  VYMYFNVWTICRNTTLVEGANLCLDLRQFGIVTIGEEKKICTASENFLRMCESTELNMTFHLFIVALAGA
  GAAVIAMVHYLMVLSANWAYVKDACRMQKYEDIKSKEEQELHDIHSTRSKERLNAYT
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC023461
• ORF Size: 803 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC023461, AAH23461
• RefSeq Size: 2780 bp
• RefSeq ORF: 803 bp
• Locus ID: 234267
• Cytogenetics: 8 B1.3
• Gene Summary: Involved in neuronal differentiation, including differentiation and migration of neuronal stem cells. Plays a role in neuronal plasticity and is involved in neurite and filopodia outgrowth, filopodia motility and probably synapse formation. Gpm6a-induced filopodia formation involves mitogen-activated protein kinase (MAPK) and Src signaling pathways. Conflictingly, PubMed:22162747 reports that induced cellular protrusions are simple membrane-wrapped tubules without actin or tubulin-based cytoskeletons and with Gpm6a gliding along membrane edges indicative for a function in actin-independent membrane deformation. May be involved in neuronal NGF-dependent Ca(2+) influx. May be involved in regulation of endocytosis and intracellular trafficking of G-protein-coupled receptors (GPCRs); enhances internalization and recycling of mu-type opioid receptor.[UniProtKB/Swiss-Prot Function]