Gadd45a (NM_007836) Mouse Tagged ORF Clone

CAT#: MG201332

Gadd45a (tGFP-tagged) - Mouse growth arrest and DNA-damage-inducible 45 alpha (Gadd45a)

Product Images

Circular map for MG201332

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Gadd45a
• Synonyms: AA545191; Ddit1; GADD45
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG201332 representing NM_007836
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTTTGGAGGAATTCTCGGCTGCAGAGCAGAAGACCGAAAGGATGGACACGGTGGGCGATGCCCTGG
  AGGAAGTGCTCAGCAAGGCTCGGAGTCAGCGCACCATTACGGTCGGCGTGTACGAGGCTGCCAAGCTGCT
  CAACGTAGACCCCGATAACGTGGTACTGTGCCTGCTGGCTGCTGACGAAGACGACGACCGGGATGTGGCT
  CTGCAGATCCATTTCACCCTCATCCGTGCGTTCTGCTGCGAGAACGACATCAACATCCTGCGGGTCAGCA
  ACCCGGGTCGGCTAGCTGAGCTGCTGCTACTGGAGAACGACGCGGGCCCGGCGGAGAGCGGGGGCGCCGC
  GCAGACCCCGGACCTGCACTGTGTGCTGGTGACGAACCCACATTCATCACAATGGAAGGATCCTGCCTTA
  AGTCAACTTATTTGTTTTTGCCGGGAAAGTCGCTACATGGATCAGTGGGTGCCCGTGATTAATCTCCCGG
  AACGG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG201332 representing NM_007836
  Red=Cloning site Green=Tags(s)
  
  MTLEEFSAAEQKTERMDTVGDALEEVLSKARSQRTITVGVYEAAKLLNVDPDNVVLCLLAADEDDDRDVA
  LQIHFTLIRAFCCENDINILRVSNPGRLAELLLLENDAGPAESGGAAQTPDLHCVLVTNPHSSQWKDPAL
  SQLICFCRESRYMDQWVPVINLPER
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007836
• ORF Size: 495 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007836.1, NP_031862.1
• RefSeq Size: 1224 bp
• RefSeq ORF: 498 bp
• Locus ID: 13197
• UniProt ID: P48316
• Cytogenetics: 6 C1
• Gene Summary: Might affect PCNA interaction with some CDK (cell division protein kinase) complexes; stimulates DNA excision repair in vitro and inhibits entry of cells into S phase. In T-cells, functions as a regulator of p38 MAPKs by inhibiting p88 phosphorylation and activity.[UniProtKB/Swiss-Prot Function]