Ncor2 (NM_001253905) Mouse Untagged Clone

CAT#: MC229724

Ncor2 (untagged) - Mouse nuclear receptor co-repressor 2 (Ncor2), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ncor2
• Synonyms: N-CoR; SMRT; SMRTe
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229724 representing NM_001253905
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAGGATCCACACAGCCTGTGGCACAGACATGGCGGGCTGCTGAGCCCCGCTACCCACCCCATGGCA
  TCTCCTACCCGGTGCAGATAGCCCGGTCCCACACGCCTCTGTACAACCAGCCGTCTGACACACGCCAGTA
  CCATGAAAACATCAAAATAAACCAGGCGATGCGGAAGAAGCTGATCTTGTACTTTAAGCGGAGGAACCAC
  GCGCGCAAGCAGTGGGAACAGCGCTTCTGCCAGCGCTATGACCAGCTCATGGAGGCGTGGGAGAAGAAGG
  TAGAGCGCATAGAGAACAATCCGCGAAGGAGGGCCAAGGAGAGCAAGGTGAGGGAGTACTACGAGAAACA
  GTTCCCGGAGATCCGCAAGCAGCGGGAGCTGCAGGAGCGCATGCAGAGCAGGGTGGGCCAGCGTGGCAGT
  GGGCTCTCCATGTCGGCTGCCCGCAGTGAGCATGAGGTTTCTGAGATCATTGATGGCTTGTCTGAGCAGG
  AGAATCTGGAGAAGCAGATGCGCCAGCTGGCCGTGATCCCGCCCATGTTGTACGACGCGGACCAGCAGAG
  GATCAAGTTCATCAACATGAATGGACTCATGGATGACCCCATGAAGGTCTACAAGGACCGTCAGGTTACC
  AACATGTGGAGCGAGCAGGAGAGGGACACCTTCCGTGAGAAGTTTATGCAGCACCCTAAGAACTTTGGCC
  TGATTGCCTCATTCCTGGAGAGAAAGACGGTCGCTGAGTGTGTCCTCTATTACTACCTGACCAAGAAGAA
  TGAAAATTACAAGAGCTTGGTGAGGCGGAGCTATCGGCGCCGTGGCAAGAGCCAGCAGCAGCAGCAGCAG
  CAACAACAGCAGCAGCAGCAGCAGATGGCACGGAGCAGCCAGGAGGAGAAGGAGGAGAAGGAGAAGGAGA
  AGGAGGCCGACAAGGAGGAAGAGAAGCAGGATGCGGAGAACGAGAAGGAAGAACTCAGCAAGGAGAAGAC
  AGACGACACTTCTGGCGAGGACAACGATGAGAAAGAGGCCGTGGCCTCCAAAGGCCGCAAAACTGCCAAC
  AGCCAAGGCCGCCGCAAAGGCCGTATCACGCGCTCCATGGCCAACGAGGCCAACCATGAGGAGACAGCCA
  CCCCACAGCAAAGTTCAGAGCTGGCTTCCATGGAGATGAACGAGAGTTCTCGCTGGACTGAGGAAGAGAT
  GGAGACAGCAAAGAAAGGCCTCCTGGAACATGGGAGGAACTGGTCAGCCATTGCCCGTATGGTGGGCTCC
  AAGACCGTGTCCCAGTGTAAGAACTTCTACTTCAACTACAAGAAGAGGCAGAACCTGGACGAAATCCTTC
  AGCAGCACAAGCTAAAGATGGAGAAGGAGAGGAACGCTCGGAGGAAGAAGAAGAAGACCCCAGCTGCGGC
  GAGCGAGGAGACAGCCTTCCCACCTGCCGCTGAGGACGAAGAGATGGAAGCATCAGGCGCAAGTGCCAAT
  GAGGAAGAGCTGGCGGAGGAGGCAGAAGCCTCACAGGCCTCTGGGAATGAGGTTCCCAGAGTTGGGGAGT
  GCAGTGGCCCAGCTGCTGTCAACAACAGCTCTGATACTGAGAGTGTCCCATCCCCGCGTTCAGAAGCCAC
  GAAGGACACTGGGCCTAAACCCACTGGCACTGAAGCATTGCCCGCTGCCACCCAGCCACCTGTTCCTCCT
  CCAGAAGAACCGGCAGTAGCCCCTGCTGAGCCCTCCCCAGTCCCTGATGCCAGTGGCCCACCATCCCCAG
  AGCCTTCCCCATCACCTGCCGCACCCCCGGCTACTGTGGACAAGGATGAACAAGAAGCCCCGGCTGCTCC
  AGCTCCCCAGACAGAGGATGCCAAGGAGCAGAAGTCTGAGGCCGAGGAGATCGATGTGGGAAAGCCAGAG
  GAGCCCGAGGCCTCTGAGGAGCCCCCGGAGAGTGTAAAGAGTGACCACAAGGAGGAGACCGAGGAAGAGC
  CTGAAGACAAAGCCAAGGGCACAGAGGCCATTGAAACTGTGTCTGAGGCACCACTTAAGGTGGAGGAGGC
  TGGTAGCAAGGCAGCTGTGACCAAGGGTTCCAGCTCAGGTGCCACCCAGGACAGTGACTCCAGTGCCACC
  TGCAGTGCCGATGAGGTGGACGAACCCGAAGGAGGTGACAAGGGCAGGCTGCTGTCACCAAGGCCCAGCC
  TCCTCACCCCGGCTGGAGATCCCCGGGCCAGTACCTCGCCCCAGAAGCCGCTGGACCTGAAGCAGCTGAA
  GCAGCGAGCAGCCGCCATCCCCCCTATCGTCACCAAGGTCCATGAGCCCCCCCGGGAGGACACAGTACCC
  CCAAAGCCAGTTCCCCCTGTGCCTCCACCCACGCAGCACCTACAGCCAGAGGGTGACGTGTCTCAGCAGT
  CGGGAGGAAGTCCACGTGGCAAGTCCCGCAGCCCAGTGCCTCCTGCCGAGAAAGAGGCAGAGAAACCCGC
  ATTCTTTCCGGCTTTCCCAACTGAGGGCCCAAAGCTACCGACTGAGCCCCCACGCTGGTCATCGGGCCTG
  CCCTTCCCCATCCCTCCACGGGAGGTGATCAAGACTTCCCCACACGCCGCTGACCCCTCTGCCTTCTCCT
  ACACACCCCCCGGTCACCCGCTGCCTCTGGGCCTCCACGATAGTGCCCGGCCCGTCCTGCCACGTCCCCC
  CATCTCTAACCCCCCACCCCTCATCTCCTCTGCCAAGCATCCCGGCGTACTTGAGAGGCAGCTGGGTGCC
  ATCTCCCAGCAGGGGATGTCAGTCCAGCTTCGTGTGCCTCACTCAGAGCATGCCAAGGCCCCCATGGGCC
  CTCTCACCATGGGGCTGCCCCTTGCCGTGGACCCTAAGAAGCTGGGGACAGCACTGGGTTCCGCCACCAG
  TGGAAGCATCACCAAGGGCCTCCCCAGTACCCGGGCTGCAGACGGCCCCAGCTACAGAGGCTCTATCACC
  CACGGCACGCCCGCAGACGTCCTCTACAAGGGTACCATCAGCAGGATCGTCGGTGAGGACAGCCCAAGTC
  GCCTTGACCGGGCACGAGAGGACACCCTGCCCAAGGGCCATGTCATCTATGAGGGCAAGAAAGGCCACGT
  CCTATCCTATGAAGGTGGTATGTCCGTGTCACAGTGCTCTAAGGAGGATGGAAGGAGCAGCTCGGGCCCA
  CCCCATGAGACTGCCGCCCCTAAACGCACCTATGACATGATGGAGGGCCGTGTAGGCAGGACTGTCACCT
  CAGCCAGCATAGAGGGACTCATGGGCCGCGCCATCCCTGAGCAGCACAGCCCCCACCTCAAGGAGCAGCA
  TCACATCCGAGGCTCCATCACGCAAGGCATCCCGAGGTCCTATGTGGAGGCGCAGGAGGACTACTTACGG
  CGGGAGGCCAAGCTCTTGAAGCGAGAAGGGACACCACCACCCCCACCACCACCTCGGGACCTGACTGAGA
  CCTACAAGCCCCGGCCCCTGGACCCTCTGGGTCCCCTGAAGCTGAAGCCGACTCACGAGGGTGTGGTAGC
  AACTGTGAAGGAGGCGGGCCGCTCTATCCATGAGATCCCGAGAGAGGAGCTGCGCCGCACACCTGAGCTA
  CCCCTGGCACCACGGCCTCTGAAGGAGGGTTCCATCACCCAGGGCACCCCACTCAAGTACGACTCTGGGG
  CACCCTCCACTGGCACCAAGAAACACGACGTGCGCTCCATCATCGGCAGCCCCGGCCGGCCTTTCCCTGC
  CCTGCACCCGCTGGACATAATGGCTGACGCCCGGGCACTGGAGCGTGCCTGCTATGAAGAGAGTCTGAAG
  AGCCGGTCAGGGACCAGCAGTGGTGCAGGGGGCTCCATCACACGTGGGGCTCCAGTCGTCGTGCCTGAAC
  TGGGCAAGCCACGGCAAAGCCCACTGACTTACGAAGACCACGGGGCACCCTTCACCAGTCACCTGCCACG
  TGGCTCCCCTGTGACCACGAGGGAGCCCACGCCACGCCTTCAGGAAGGCAGCCTCCTATCCAGCAAGGCG
  TCCCAGGACCGGAAGCTGACATCTACACCCCGGGAGATCGCCAAGTCCCCACACAGCACTGTGCCCGAGC
  ACCACCCTCACCCCATCTCCCCCTATGAGCACTTGCTCCGGGGCGTGACTGGTGTGGACCTGTACCGTGG
  TCACATCCCATTGGCCTTTGACCCCACCTCCATACCCCGAGGGATCCCTCTGGAAGCAGCAGCCGCAGCC
  TACTACCTGCCCCGGCACTTGGCCCCCAGCCCCACCTACCCACACCTGTACCCACCTTACCTCATCCGCG
  GCTACCCTGACACGGCGGCCCTGGAGAACCGCCAGACCATCATCAATGACTACATCACCTCGCAGCAGAT
  GCACCACAACGCTGCCTCCGCCATGGCCCAGCGTGCTGACATGCTGAGGGGTCTGTCACCGCGAGAGTCC
  TCGCTGGCCCTCAATTATGCCGCTGGCCCAAGAGGCATTATCGACCTGTCCCAAGTGCCACACCTGCCCG
  TGCTGGTGCCACCAACGCCAGGCACCCCTGCCACCGCCATCGACCGCCTTGCCTACCTCCCCACTGCGCC
  CCCACCCTTCAGCAGCCGCCACAGTAGCTCACCGCTGTCCCCAGGAGGCCCCACTCACCTAGCTAAACCA
  ACTGCCACATCTTCATCGGAGCGGGAACGGGAACGTGAGCGGGAACGAGACAAGTCCATCCTCACGTCTA
  CCACTACAGTGGAGCATGCACCCATCTGGAGACCTGGTACGGAGCAGAGCAGCGGGGCTGGGGGCAGCAG
  CCGCCCCGCCTCCCACACCCACCAGCACTCGCCCATCTCCCCCCGGACCCAGGACGCCTTGCAGCAGAGG
  CCCAGTGTGCTGCACAACACGAGCATGAAGGGCGTGGTCACCTCCGTGGAACCCGGCACGCCCACGGTCC
  TGAGGTGGGCCAGGTCCACCTCCACCTCTTCGCCTGTCCGCCCAGCTGCCACATTCCCACCTGCCACCCA
  CTGCCCACTTGGTGGCACCCTTGAAGGGGTCTACCCTACCCTCATGGAGCCCGTCCTGTTACCCAAGGAG
  ACCTCTCGGGTCGCCCGGCCCGAGCGGCCCCGTGTGGACGCTGGCCATGCCTTCCTCACCAAACCCCCGG
  CCCGGGAGCCCGCCTCCTCACCCAGCAAGAGCTCCGAGCCCCGATCCCTAGCACCCCCCAGCTCCAGCCA
  CACAGCCATCGCCCGCACCCCAGCAAAGAACCTTGCACCCCACCATGCCAGTCCGGACCCGCCGGCGCCC
  ACCTCGGCCTCAGATCTGCACCGAGAAAAGACTCAAAGTAAACCCTTTTCCATCCAGGAATTGGAACTCC
  GTTCTCTGGGTTACCACAGTGGAGCTGGCTACAGCCCCGATGGGGTGGAGCCCATCAGCCCGGTGAGCTC
  CCCCAGCCTGACCCACGACAAGGGGCTCTCCAAACCTCTGGAAGAGCTAGAGAAGAGCCACTTGGAAGGG
  GAGCTGCGGCACAAGCAGCCAGGCCCCATGAAGCTCAGCGCGGAGGCTGCCCATCTCCCACATCTGCGGC
  CACTGCCCGAGAGCCAGCCCTCATCTAGCCCACTCCTCCAGACTGCCCCAGGCATCAAAGGTCACCAGAG
  GGTGGTCACCTTGGCTCAGCACATCAGTGAGGTCATTACGCAGGACTACACGCGCCACCACCCGCAGCAG
  CTCAGTGGCCCCCTTCCCGCCCCTCTCTACTCCTTTCCCGGAGCCAGCTGCCCTGTCCTGGATCTTCGCC
  GCCCACCCAGTGACCTCTACCTCCCACCCCCCGACCATGGCACCCCAGCCCGGGGATCCCCCCACAGTGA
  AGGGGGCAAAAGGTCCCCAGAACCCAGCAAAACATCGGTCCTGGGCAGCAGCGAGGATGCCATTGAGCCT
  GTGTCCCCACCAGAGGGCATGACTGAGCCAGGACATGCTCGGAGCACTGCGTACCCACTGCTGTATCGAG
  ACGGGGAACAGGGCGAGCCCAGGATGGGCTCTAAGTCTCCAGGCAACACCAGCCAGCCGCCAGCCTTCTT
  CAGTAAGCTGACTGAGAGCAACTCCGCCATGGTGAAGTCGAAGAAGCAGGAGATCAACAAGAAACTCAAC
  ACCCACAACCGGAACGAGCCAGAATACAATATTGGCCAGCCTGGGACGGAAATCTTCAACATGCCCGCCA
  TCACTGGAGCAGGCCTTATGACCTGTAGAAGCCAGGCGGTGCAAGAACACGCCAGCACCAACATGGGGCT
  AGAGGCCATTATTAGAAAGGCACTCATGGGTAAATATGATCAGTGGGAAGAGCCCCCGCCGCTCGGCGCC
  AATGCTTTTAACCCTCTGAATGCCAGCGCCAGTCTGCCCGCTGCTGCTATGCCCATAACCACTGCTGACG
  GACGGAGTGACCACGCACTCACCTCGCCAGGTGGAGGTGGGAAAGCCAAGGTCTCTGGCAGACCTAGCAG
  CCGAAAAGCCAAGTCGCCAGCACCAGGCCTAGCGTCCGGAGACCGACCCCCTTCTGTCTCCTCAGTACAC
  TCAGAGGGGGACTGCAATCGCCGAACACCACTCACCAACCGTGTGTGGGAGGACCGGCCCTCATCTGCAG
  GGTCCACGCCATTCCCCTACAACCCTTTGATTATGAGGCTACAGGCAGGTGTCATGGCCTCCCCGCCCCC
  ACCTGGCCTTGCGGCAGGCAGCGGGCCCCTAGCTGGTCCCCACCACGCCTGGGATGAGGAGCCCAAGCCA
  CTGCTGTGTTCACAGTATGAGACACTCTCGGACAGCGAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001253905
• Insert Size: 6762 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001253905.1, NP_001240834.1
• RefSeq Size: 8146 bp
• RefSeq ORF: 6762 bp
• Locus ID: 20602
• UniProt ID: Q9WU42
• Cytogenetics: 5 G1.1
• Gene Summary: Transcriptional corepressor. Mediates the transcriptional repression activity of some nuclear receptors by promoting chromatin condensation, thus preventing access of the basal transcription. Isoform 1 and isoform 5 have different affinities for different nuclear receptors. Involved in the regulation BCL6-dependent of the germinal center (GC) reactions, mainly through the control of the GC B-cells proliferation and survival. Recruited by ZBTB7A to the androgen response elements/ARE on target genes, negatively regulates androgen receptor signaling and androgen-induced cell proliferation.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) has an additional exon in the 5' UTR and has multiple coding region differences, compared to variant 1. The resulting isoform (3) has identical N- and C-termini, but has some internal segment differences, compared to isoform 1.