Frem1 (NM_001198811) Mouse Untagged Clone

CAT#: MC229714

Frem1 (untagged) - Mouse Fras1 related extracellular matrix protein 1 (Frem1), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Frem1
• Synonyms: BC037594; crf11; D430009N09; D630008K06; eye; eyes2; heb; qbrick
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229714 representing NM_001198811
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCACTCTCCAGGCTGCACTGGGCCCAAGGCTCAGTGGTTCTTGCTGCTCCAGCTGCTTCTGCTGCACC
  TGGACCGGGTCAGTGCCACCTTCATCAGCATCAACCGGGGCCTGAGGGTGATGAAGGGTAGTTCTGCCTT
  CCTGTCTGGAGATCACCTGAGGGTTGCAGTCCCTAAAGAAAAGGACGCCTGCAGACTGGAGGTGGTGATG
  AACGAGCCCGTAACCCAGAGGGTTGGGAAACTCTCTCCACAGGTCTTTGATTGCCATTTTCTTCCCAACG
  AAGTGAAGTATGTTCACAATGGATGCCCAATTCTGGATGAAGACTCTGTGAAGCTTAGACTTTACAGGTT
  TACTGAAACAGACACCTTCATGGAAACCTTTCTCTTGCGGGTTTATCTTGTGGAACCAGACTGTAACATC
  ATCCGTATGAGCAGTAATGTGTTGGAAGTGACTGAATTCTATGGCTTATCGCAAGCCATTGATAAGAACC
  TGCTCCAGTTTGATTATGATAGGACTGCCAGCCTGGATTGCACCATCAGACTAGACCCACTGAGGACTCA
  GCTGCCAGCTCACGGCAAGCTGGTCGTGGTGAATCGTAAGTCAGAGGGGCCTCGTGGAGATCAGCCACAC
  AGCTTCTTCTCTGAGACAGAGCTAGGGGCTGGACTGAAGTGTCCAGATGGAAGCTGTGCCCTGGAATTGA
  AGCAAGTGGCAAGTCTGAAAGTGAGCTGTGAGGAGTTTCTGCTGACGGGGTTTCACTACCAACACATGCA
  GCCCCCTTCACCCAACATTGATTACATCCCCATTCAGTTGGATCTCACCGACAGAAGGAGCAAAACCGTA
  TACAAGTCAGAGAGTGCGTGGTTGCCTGTCTATATCAGAGTTGGCATTCCTAACCAGGTCCCAAGGGCTG
  CATTTATGGCCATGTTTGTTCTGGAGGTGGACCAGTTCATACTGACCCCTCTGACAACCTCCGTTCTGGA
  CTGTGAGGAGGATGAGACTCCGAAACCCTTGTTGGTGTTCAACATCACTAAAGCCCCACTCCAGGGCTAT
  GTGACCCACCTGCTGGATCATACCAGACCCATCTCTTCATTCACCTGGAAGGATCTCAGTGACATGCAAG
  TTGCCTACCAGCCACCAAACAGCAGCCACCCTGAGAGGAGACATTACACGATGGAGCTGGAGGTGTACGA
  CTTCTTCTTTGAGAGGAGTGCCCCCATCACTGTCCACATCTCCATCAGAACAGCAGACACGAATGCACCA
  CGGGTGTCTTGGAATACAGGTTTGAATCTCCTAGAGGGGCAGTCTCGGGCCATCACTTGGGAACAATTTC
  AGATCGTTGACAATGATGACATTGGTGCTGTGCAGTTGGTCACCATTGGTGGCCTGCAGCATGGACGGCT
  TACAGTACGAGAGGGGAAAGGATTTCTCTTCACCGTGACTGACCTTCAAGCTGGAGTTGTCCGCTATCAT
  CACGATGACAGTGATACCACCAAAGACTTTGTGGCTTTCAGGATATTCGATGGCCACCACAGCAGCCACC
  ACAAGTTTCCTATCAACATCTTGCCTAAAGATGACAGCCCCCCATTCCTCATCACCAATGTCGTGATTGA
  ACTAGAAGAGGGGAAGACCATCTTGATCCAAGGGTCTATGTTGAGAGCTTCAGATATGGACTCCAGTGAT
  GACTACATCTTCTTTAATATCACAAAGTTTCCACAGGCTGGGGAGATCATGAAGAAGCCAGGGCCAAGAC
  TGATAGGGTATCCTGTCCCTGGCTTCCTGCAGAGAGACCTGTTCAGTGGAATCATTTACTATCGTCACTT
  TGGTGGAGAAATCTTTGAAGATTCCTTTGAATTCGTACTGTGGGACAGCCATGAGCCTCCAAATCTCTCA
  GTGCCACAGGTGGTGACAATTCATATCACTCCAGTAGATGACCAGCTTCCTAAAGAAGCTCCTGGCATTT
  CCCGGCATTTGGTTGTCAAGGAAACAGAGGTGGCCTACATCACTAAAAAGCACCTACATTTCCTAGACAT
  GGAATCCCGTGATGGGGAACTCATCTACACAGTAACACGCCCTCCATGTTTCTCCTTCAGCCACAGACAC
  TTGGATGCTGGGAAGCTATTCATGGTGGACAGCATACCAAAGCTAACCAAAAATCCGACAGCCCCAGGGC
  TAAGTTCATTTACTCAGCATGCCGTGAACCATATGAAAGTGGCCTACATGCCACCCATGCAAGATATAGG
  CCCCTCCCCTCGACATGTGCAGTTCACAGTGTCTGTCAGTAACCAGCATGGAGGTGCTTTGCATGGAATC
  TGCTTCAACATCACAGTTCTCCCAGTGGACAACCAGGTTCCCGAGGTATTCACCAACACCCTGAGAGTGG
  TCGAGGGCGGTCAGTGCACCATCAGCACAGAGCACATTTTGGTTTCTGATGTGGACACCCCGCTAGACAG
  CATCAGCCTCTCTCTTAAGGAGAGGCCTCTGCATGGCGGGGTAGAGCTGGACGGATTTCCTCTAAACCCA
  AGAGGCACATTTTCTTGGCGAGACCTCAATACCTTAAAAGTTTGGTATCAACATGATGGATCTGAGGTTC
  TTCAGGATGAGATATTTTTGGAGGTCACAGATGGCACAAATTCAGCAGCATTTGTTCTCCACATTGAGGT
  GTTCCCTGTGAATGATGAGCCACCCATTCTGAAGGCTGACCTCATCCCCATGATGCACTGCTCTGAGGGT
  GGAGAGGTGACCATCACCCCTGAATACATTTCCGCTACTGATGCAGACAGCGATGACTTAGAACTGCTGT
  TTCTGATTGCCCGAGAGCCTCAGCATGGAGTGGTGAGAAAGGCTGGACTTCACGTGGACAGGTTCTCTCA
  GGGAGATGTCATCTCAGGGGCTGTGACATACAAACATACAGGTGGCGAGATTGGCCTTGAACCTTGTTCT
  GACACTGTTGTATTGGTGGTTTCGGATGGTGAAGCTGACCCTTTGATGAACGGCTGTTGCTACGATGGAC
  CTGATTCATCTGTTCCTCTTCATAAGTCCTTCCCCACATACCAGCTCAACATCACAGTACATCCAGTCGA
  CAACCAGCCACCTTCAATTATAATAGGTAGGATGCTCACAGTGGATGAAGGCTTCTCAGCAGCCCTTACT
  ACTCATCACTTGACTGTTGTTGATTGGGACACTGCCCCGGATGACTTAAAATTCATGTTGGCTTCTCAGC
  CCCAGTTTGGCTACTTAGAAAACGCACTGCCTTCTGCAGGCTTTGAGAAAAGTAACATCGGCATAAGGAT
  AGCCTCATTCCAGTGGACAGACATGAAGGCTTCACACATTAACTATGTGCAGTCCAGGCACCTGAGAGTG
  GAACCAACTGCTGACCAGTTCACAGTATATGCCACAGACGGGAAGCATCGCTCCTTGGAGACAACATTTC
  ACGTTATCATCAACCCCACAAACGATGAAGCTCCTGACCTTGCAGTACAGAACATCACTGTGTATGAGGG
  GCACATGGTAGAACTGGATTCTTCCATCATCAGTGCTACTGACAGGGATATTCCCAAGGACCCCTTGCTC
  TTCAGCATCGCTCTCAAGCCCCAGCACGGCCTGCTCGTCGATGCTGCCATCAGCAAGGACTCCCATCAGA
  TCAAGCAGCTGCAACACGAAATCCACAGCTTCTCCGTGGACCTTCTCAAGAATGGAATGAAGCTGGTCTA
  TGCCCACGACGACTCAGAGAGCTCTGCTGATAACTTCGTGATCCAGTTGTCAGACGGGAAACATAAAATC
  CTTAAAACCATCTCAGTAAACATTACCCCAGTGAATGACGAGACACCAACGCTAAGCAAGAAGGCTGAAA
  TCTCAATGGCTGTGGGCGATACTCGCGTCCTGTCTAGTGCTGTTCTTTCAGCCACAGATAAAGACTCGCC
  CAGAGAGAAGATTCACTATGTATTTGAAAGACTTCCCCAAAATGGACAACTTCAACTTAAGATAGGGAGG
  GACTGGGTCCCTCTCTCCACTGGCATGCAGTGCACCCAGGAGGATGTGGACCTGAACTTGCTAAGATACA
  CGCATGCTGGTAAAACGGACTCCCAGGATGGAGACAGCTTCACCTTCTACCTCTGGGATGAGGACAACAG
  GTCACCTGCATTTGACTGTCACATCATCATTGAAGACATGGGCAAAGGTGATATTGTCATTCATGCAAAA
  CCACTCGTGGTAGTCAAAGGTGACAGAGGTCTCTTGACGACTGCCACCCTTCTGGCTGTGGATGGAGCAG
  ACAAGCCTGAGGAACTGCTCTACCTCATCACCTCCCCACCACGGCATGGCCAAGTGGAGTACGTCCACTC
  TCCTGGAGTCCCCATTGCCAGCTTCAGCCAAATGGACATAGCAGGACAGACAGTGTGCTACATACACAAG
  AGCAGGACAGCTGTCCCCACTGACAGCTTCAGATTTACCATCAGCAATGGACTGCAGACCCAGCGTGGGG
  TGTTTGAAATCACACTGCAGACTGTGGACAGCGCCTTGCCTGTGCTGACCAAGAACAAAAGGCTGAGGCT
  AGCCGAAGGGGCCATGGGCCTCCTGTCCGCTGATCACCTTCAGCTGACTGACCCCGACACACCTCCAGAG
  AACCTGACCTTCTTTCTGGCTCAGCTCCCACGCCACGGGTACCTCTTCCTGAGAGGGAAAGCACTCCAAC
  ACAACTTCACCCAGCGAGATGTGGACAGCGGGGGCGTGGCCTACCAGCACTCAGGAGGCGGCGCCCGGGA
  GGACTATTTTACTTTTCTAGCCACAGACAGGAAGAACCAAGGCTTTGTTGTGGATGGGAAAGTTCAGAAA
  GAGCCTGTTCGCTTCACAATCCAGGTGGATCAGCTGGACAAGGCAGCGCCCCGTATCACACACTTGCACT
  CCCCTACTCAAGTGGGGCTCTTGAAAAATGGCTGCTATGGGATTTACATCACTTCCCGTGTGCTGAAGGC
  ATCAGACCCTGACACAGAGGATGACCAGATCATCTTTAAGATTTTACGAGGCCCATTGTACGGACGTCTG
  GAGAACACAACAACAGGTGAATTCATCCACGAGCGATTTAGCCAGAAGGACTTAAGCCATAAGACCATCC
  TTTACATCATAAACCCTTCTTTGCAAGTGACTTCCGATATCCTGGAATTTCAGGCCATGGACCCCACAGG
  GAACACTGCCACTCCCCAGAGCTTGGACTTGAGATGGTCGTATATTGAATGGGCCCAGACTGCATATGAG
  GTTTGTGAGAATGTGGGCTTATTGCCCTTGGAAGTTACCAGAAGGGGATATCCCATGGACTCAGCCTTTG
  TGGGTGTAGAGGTTAACCAAGTGTCAGCTACAGTTGGAAAAGATTTTACTGTCACTCCATCCAAACTGCT
  TCAATTTGACCCAGGAATGTCAACTAAGATGTGGAATATAGCAATTACCTATGACGGATTAGAGGAAGAT
  GATGAGGTCTTTGAAGTAATTCTGAACTCCCCTGTGAATGCGGTTCTTGGCACGCAGACAAAAGCGGCGG
  TGAAAATTTTGGACTCAAAAGGAGGACGGTGCCATCCTTCAAATTCCTTCAACCAAAGCAAGCACAGCAC
  GTGGGGGAAGGGCCCTTGGCATCCGCTGCCCTCAGGCTCATCCTCGCTCACCACTTCAGGTTCTCCTCTT
  CTGGAAAGGCCTCCTCCATCTTTCACCAGTGGAGATGCTCTTCAGGGTTTTGGTCTCACCGACCTCACTC
  AAAGGAAGACAATGACCCAGGGGAATGGCAAATCAGTTCTCCCATCCTCTGTTTGTAGAAATGGGACAGA
  CACCATCTACAATTACCATGGTATAGTTTCCTTGAAACTAGAGGGCGACAGATTCTCAGCTCACAAACGG
  AAGGCCAAAATATCCATTGTTAGTCAGCCACAGAGGACAATCAAGGTGGCAGAGCTGCCTCTAGCTGATA
  AGGTGGAATCCACAACTGATTTGCACTTTCTCAGACAGGGTCTGCGGCCCTTGTTTCCAAAGAATTGTTC
  TGTGGACTTAAAGGGACTCTTTCATTTTGAAGAAAGCACCCACAGATTGTATCAATGTGATGGGATCTCC
  TGGAAAGCCTGGAGCCCCCAAACCAAGGGGCTGGAGGACAGATCCTGCCCAGGTGGATGGCTCCTTCATT
  CTGGCTACTGTCACATCTTGGTCACAAGACAGAAAGGCACCTGGACCACAGCTACCCGAGCTTGCAGAGA
  ACAGCACCAGGGCGACCTTGTGACCGTACTCTCCAGAAGGCATATGCAGTGGCTATGGGCGATGAGTGGA
  AGAAAACCCTTCTGGATAGGCTTGAAGAACCAACCGAGGACTGGCCACTGGGAGTGGATCGGAGGTGAAC
  CTGTTGCTTTTACCAACTGGAGGAGAGGGGCCCCTCTGCACCCAAAACCTGGAAAGAACTGTGCTTTGGT
  TCAGAAAAGAGGACAATGGCAGACAAAGAACTGTAGCAAAGGCAAGGCTCACAATTTTGTGTGCTCAAGG
  AAACTCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001198811
• Insert Size: 6519 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001198811.1, NP_001185740.1
• RefSeq Size: 9524 bp
• RefSeq ORF: 6519 bp
• Locus ID: 329872
• UniProt ID: Q684R7
• Cytogenetics: 4 C3
• Gene Summary: Extracellular matrix protein that plays a role in epidermal differentiation and is required for epidermal adhesion during embryonic development.[UniProtKB/Swiss-Prot Function]