Nrap (NM_001286552) Mouse Untagged Clone

CAT#: MC229639

Nrap (untagged) - Mouse nebulin-related anchoring protein (Nrap), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Nrap
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229639 representing NM_001286552
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAGGGATTGACGGCAAAGAAGATGGCGAGCCATTTAAATCCGTTCTCCATTGGGACATGAAGTCCA
  AAGCTGGAGCAGGGGCAGCTAGCCGGCTGATGAATGAGAGAGACTACTGGCCAGGGTATGCAGAAGGGAA
  CACTTGGTGTCCAGGAGCTCTGCCGGACCCTGAGATTGTAAGGATGGTTGAAGCTCGACAGTCTCTCGGT
  GAGCAGGGGTACACAGAAGACCGTGAACAGCAGCAGGGCAAGGGGAGCTTTCCAGCCATGATCACCCCTG
  CCTACCAAAGGGCCAAGGCAGCCAACCAGCTGGCCAGCCAGGTACAATACAAGAGAGGCCACGATGAGCG
  GGTCTCCACATTCACTCCAGTGGCGGACACGCCCGAGCTGCTCCGTGCCAAAGCTGGGGGACAGCTTCAA
  AATGACGTGAGGTACACAGAAGACGGTGGGCAGCAGAGAGGGAAAGGCAGTTTTCCTGCTATGATCACAC
  CAGCTTATCAGATAGCCAAAAGGGCCACTGAGCTGGCGAGTGATGTGAGGTACCATCAACAATATCATAG
  AGAAATGAAGGGAATGGCTAGTCCTGTCGGAGCCGAGGGTGGGATGACAAAGGACTCTGTAGACCGATGT
  GGCCAGGTTTACTCAGAAGAGTGTGATGAACCCAGAGGAAAGGGCAGCTTCCCGGCCATGATCACCCCAG
  CGTATCAGAACGCCAAGAAGGCCAATGAACTTGTTAGTGACATAAAATACCGGCAGGACTTCCATAAGAT
  GAAAGGTGCAGCGCATTTTCACTCCCTTGCAGCCCAGGACAACTTGGTTCTTAAGCGAGCTCAGAGTGTG
  AGCAAGCTTGTGAGTGAGAATAAATATAAAGAAAACTATCAGACCCAGCTGAGAGGCCACTATGACGGAG
  TCGGCATGGACAGACGCATGCTCCACGCTCTCAAAGTTGGCAGCCTGGCTAGCAATGTTGCCTACAAGGC
  TGATTATAAACATGACGTTGTGGATTACAACTACCTGGCCACTGCCACTCCATTCTACCAAACAACGATG
  AGGCTGGTTCCCCTGAAGGATGTCAACTACAGGCAGAACATTGACAGAATGAAGTTCAGCTCGGTGACAA
  ACACGCCACAGATTGTTCAAGCTAAGATCAACGCCCAGCAGCTGAGCCATGTGAATTACCGAGCTGACTA
  CGAGAGAAACAAGCTGAACTACACATTGCCCCAGGACGCCCCTCAGCTGCTGAAGGCCAAGGCCAATGCA
  GAACTGTTCAGCGAGGTGAAGTACAAAGAAGGCTGGCAGAAGACAAAGGGGAAAGGCTTTGAGATGAAGC
  TGGACGCCATGTCTCTGTTGGCCGCCAAAGCCTCGGGGGAGCTTGCTAGCAGCGTTAAATACAAAGAAGA
  ATATGAGAAAATGAAGGGCAGAGCCCTGGGAGCCACAGACTCTAAGCTTCTGCATTCTCTGCAGGTGGCC
  AAGATGAGCAGCGAGGTTGAATATAAGAAAGGGTTTGAGGAGAGTAAAACCCACTTTAACCTGCCCATGG
  ACATGGTGAACCTCAGGCACGCTAAGAAGGCCCAAGCTCTTGCCAGTGACCTTGACTACAGAAAGAAACT
  GCATGACTACACGGTGCTGCCTGAAGATATGAAAACTCAGTGGGCCAAGAAAGCCTACGGGCTGCAGAGC
  GAGCTGCAGTACAAAGCTGACCTGGCATGGATGAGGGGAGTCGGGTGGCTGACAGAGGGGAGTCTCAACC
  TGGAACAGGCGAAGAAGGCTGGACAGCTCATCAGCGAGAAAAACTACCGGCAGAGGGTGGATGAGCTGAA
  GTTCACCAGTGTGGCTGACAGCTCCCAGATGGAGCATGCCAAGAAGAGCCAGGGGCTGCAGAACGCGGTG
  GCCTACAAGGCAGGAAACGAGCAATCCGTCCATCAGTACACCATCAGCAAAGACGAGCCTCTCTTCTTAC
  GAGCCCGGGCCAATGCTGCTCAACTCAGCGAGACCCTGTACAAGAGCAGCTGGGAAAAGCAGAAGGCCAA
  AGGGTTTGAGCTGAGACTTGACTCCTTGGCATTCCTGACAGCCAAAGCCAAGAGAGACCTGGCTAGTGAG
  GTGAAATACAAAGAGGACTATGAAAGGTCCAGAGGGAAACTCATTGGGGCGAAGAGTGCACAGGGGGATT
  CGCAGATGAGCCACTCCCTGCAGATGTCGAAGCTACAGAGTGACCTGGAGTACAAGAAGGGCTTTGAAGA
  CACCAGATCTCAGTGCCACATCTCCCTTGACATGGTTCACCTCGTCCATGCTCGCCAGGCACAGCACCTA
  GCCACAGACGTAGGCTACAGGACAGCGTCTCACTGCTTCACGGCTCTGCCCACAGACATGAAGGTGGAGT
  GGGCCAAGAAGGCTTACGGCCTGCAGAGTGATAACCAATACAGGGCAGACATGAAGTGGATGAAAGGCAC
  AGGCTGGGTGGCCACCGGGTCATTACATGTGGAGCAGGCGAAGAAGGCAGGAGAACTCATTAGTGAGAAG
  AAGTACCGTCAGCACCCAGATGCTTTGAAGTTTACCAGTATTAAAGACACTCCGGAGATGGTCCAGGCCA
  GGATTAGTTATACCCAAGCAGTGGATAGACTGTACAGGGAGCAAGGGGAGAATGTGAAGCACCACTACAC
  ACAGACTGCAGACTTGCCTGAAGTCCTGCTGGCCAAACTGAACGCCATGAACATCAGCGAGACTCGCTAT
  AAGGAATCTTGGAGCCGGCTTCGAGATGGAGGATACAAGCTGAGGCTGGATGCCCTTCCATTCCAGGCAG
  CAAAGGCGTCCAGCGAAGTTATAAGTGACTACAAATACAAAGAAGCATTTGAGAGAATGAAAGGCCAGAT
  GCTCGGCTCCCGGAGCTTGGAAGATGACCTCAGCCTTGCTCATTCAGTGCACGCGACCTCGCTGCAGAGT
  GATGTGAATTATAAGAAAGGCTTTGAACACGCAAAGGCTCACTTCCATCTGCCGCTGGATATGGTGACCT
  TGGTGCATGCCAAGAAGGCTCAAACCCTGGCCAGCGACCAGGACTACAGACATCCGCTCCCCCAGCACAC
  AGTCTTGGCAGAAGATCTGAGGCTGAGCTGTGCCAAGAAAGCTCACAAGTTACAGAGTGAGAATCTGTAC
  CGCTCGGACTTGAACTTCATGCGTGGTGTTCCCTGTGTCGTTCCTGGAACACTGGAGATTGAAGGAAGAA
  AGAAAGCCTCTGAGCTCATCAGCGAGAGTAAATACCGCCAGCATCCTGGGTCATTCAAGTACACGGCCGT
  GACAGATACTCCCAACCTCCTGCATGCCAAGTACAGCAACCAGATCACCAATGAGCGTCTCTATAAAGCG
  GCTGGCGAGGACGCACGGCACCAATACACGATGACCCTTGGTCTGCCTGAGTTCATCCGAGCCAAAACCA
  ACGCAGCCAACCTGAGCGAGGCAAAATACAAGGAGGCTTGGCATAATCTTCGTGCTCAAGGCTACAAGCT
  GACGATAGACGCCCTCCCCTTCCAGGCTGCTCGGGCCTCTGGAGACATAGCCAGTGACTTTCTCTACAGG
  CACGAGTTTGTGAAGGAGCGTGGACAGCTCATTGGGGTGCGGAATGTGAGTGACGACCCGCGGCTACTGC
  ACTGCCTACGGATGGGCCAGCTGCAGAGTGAGAATCAGTACAGGAAGGAGGCGGCCAGCAGCCAAGCTCA
  GTGCCACCTGCCCATGGATATGATGTACCTGGTCCATGCCAGGAAGGCCCAGGCCCTGGCCAGTGACCAC
  GACTACCGGACACAGTGCCACGAGTTCACCGCCCTGCCTGAGGATCTGAAGATGGCCTGGGCCAAGAAAG
  CGCACGCCCTGCAGAGTGAGTTTCGCTACAAGGCGGACTTGATGGGCATGAAAGGGACAGGATGGCTGGC
  TCTGCAGTCTCCTCAGATAGAGAGTGCAAAGAAGGCTGGAGACCTAATCAGTGAGACCAAGTACCGTAAA
  AAACCAGACAGCATCAAGTTCACTACCGTGGTTGACTCTCCAGACCTCATTCATGCCAAGGAGAGCTACA
  TGCACTGTAACGAGCGCTTGTACAGGTTGGGAGATGCAGCCTCCCTGCATAGATACACCCCAATCCCAGA
  CCACCCGGATTTCACCCGAGCCCGCATGAACGCAATGCATTTGAGTGACAAAGTCTACAGGAATGCCTGG
  GAGCAGAGCCGGGCTGGTGGCTATGACTTCAGGCTGGATGCTATCCCATTCCAGACTGCACGGGTGTCCA
  GGGATATCGCCAGTGATTTCCGGTACAAAGAGGCATTTCTGAGGGACCGGGGCCTACAGATTGGGTACCG
  TAGCATCAGTGACGACCCGAGGACGACGCACTTCCTCCGAGTCGGCAGGCTCCAGAGTGACAACGAGTAC
  AGGAAGGCCTTTGCCAAAGGTCGCTCCCAGTTCCACAGTCGCGCTGACCAACCAGGCTTCCTCCAGGCCA
  AGAGGAGCCAGCAGCTGGCCAGCGATGTCCTCTATAGGCAGCCACTGCCTCAGCACACCAGCGACCCAGA
  ACAGCTGGGCCTGAAGCATGCCCGGAAAGCCCATCAGCTGCAGAGTGATGTCAAGTACAAATCTGACTTG
  AACCTGACTCGAGGTGTTGGCTGGACCCCTCCTGGCTCCTACAAAGTGGAAATGGCTCGACGGGCTGCAG
  AACTGGCCAACAGGAGGGGCCCGGGGATCCGGGGGGCTTCTGTGGAGCCAGAGGCAGCAGCGGCGCTAGG
  AGATCATCAGAGCAGGGGGGTGAACCCGGATGCCTCGGAGATCCTGCATATCCACAAGAAGAAGACTCTG
  CTGATGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001286552
• Insert Size: 4839 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001286552.1, NP_001273481.1
• RefSeq Size: 5305 bp
• RefSeq ORF: 4839 bp
• Locus ID: 18175
• UniProt ID: Q80XB4
• Cytogenetics: 19 51.8 cM
• Gene Summary: May be involved in anchoring the terminal actin filaments in the myofibril to the membrane and in transmitting tension from the myofibrils to the extracellular matrix.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) differs in its 5' UTR, lacks an in-frame exon in the central coding region, and initiates translation at a downstream start codon, compared to variant 2. The encoded isoform (3) is shorter, compared to isoform S.