Disp1 (NM_001278218) Mouse Untagged Clone

CAT#: MC229617

Disp1 (untagged) - Mouse dispatched homolog 1 (Drosophila) (Disp1), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Disp1
• Synonyms: 1190008H24Rik; DispA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229617 representing NM_001278218
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGC
  GCCC
  
  ATGGCTGTGATCAGCGGAAGTGACTCTGTGCTCCTGAGCAATGGCAGCATCTCGACCAGCACCTCCAACC
  CTAGTCCCCTCAGCCCCAGTGATGGAGACCTCCCAGCCCAGCACCTCGGACCCAGAGAAACCCCAAGAAC
  AAAAGCAAGCCCAAATGGATGCCTGCAACTTAATGGCACAGTTAAGTCATCCTTTCTGCCTTTAGACAAC
  CAAAGAACACCTCAGACGCCAACTCAATGCTGCCACCCTTGTCCATACCATCACCCTGTGAGCAGCCATA
  GCAATCACCAGGAGTGCCATCCCGAGGCTGGCCTTGCAGCCTCCCCCGCTTTGGCCTCGTGTCGCATGCA
  GCCACATTCCGAATACTCTGCATCTCTGTGTCCAAACCATTCACCCGTGTATCAGGCTGCGCACTGCCTT
  CAGCCCTCGCCATCTTTCTGCCTTCATCACCCGTGGCCTGACCATTTCCAGCATCAACCTGTACGGCAGC
  ACCTGACCATCATCAGGCCGTCCAGACCTTTCAAGTTCCCGAGAAGTTACGCAGCCCTGCTGGCTGACTG
  GCCTGTGGTGGTCCTGGGCATGTGCACGCTGCTCATCGTGGTCTGTGCCCTGGTGGGGGTCCTAGTGCCA
  GAGCTCCCCGACTTCTCGGACCCGCTGCTGGGTTTTGAACCAAGAGGGACCACGATAGGACAGAGGCTGG
  TCACGTGGAATAACATGATGCGAAACACAGGGTACAAAGCAACATTGGCCAACTATCCTTATAAGTATGC
  AGAGGAGCAAGCCCGAAGCCACCGGGATGACCGATGGTCAGACGATCACCATGAGAGAGAGAGAAGAGAA
  GTGGACTGGAACTTCCAGAAAGACAGCTTCTTCTGCGATGTTCCAAGTGATGGATACTCCAGAGTAGTGT
  TTGCTTCAGCAGGAGGGGAGACTCTGTGGAATTTACCTGCAATTAAGTCGATGTGTGATGTGGATAATTC
  CAGGATCAGATCCCACCCCCAGTTCAGCGATCTCTGCCAGAGGACCACTGCCGTCTCCTGCTGCCCGAGC
  TGGACCCTGGGGAATTACATCGCCATTCTGAATAACAGATCGTCCTGTCAGAAGATTGTTGAGCGAGATG
  TCTCTCACACGCTGAAGCTGCTCCGGACCTGCGCCAAGCACTACCAAAACGGCACCCTGGGGCCAGACTG
  CTGGGACAAGGCGGCCAGAAGGAAGGACCAGCTCAAGTGTACCAACGTGCCGCGCAAGTGTACCAAATAC
  AACGCCGTGTACCAGATTCTCCACTATCTGGTAGACAAGGACTTCATGACCCCAAAGACGGCTGACTACG
  CCGTGCCAGCTTTAAAGTACAGCATGCTCTTCTCCCCCACGGAGAAAGGGGAGAGCATGATGAACATTTA
  CCTGGACAACTTCGAAAACTGGAACTCCTCGGACGGCATCACCACCGTCACCGGCATCGAGTTTGGCATC
  AAACACAGCTTGTTCCAGGATTACCTCCTCATGGACACCGTGTACCCCGCCATCGCCATCGCCATTGTCC
  TGCTCATCATGTGTGTCTACACCAAGTCCATGTTCATCACGCTCATGACCATGTTTGCCATCATCAGCTC
  GCTCATTGTCTCCTACTTCCTCTACCGAGTCGTCTTTAACTTCGAGTTTTTCCCCTTTATGAACCTCACG
  GCGCTCATCATCCTGGTCGGGATTGGCGCGGACGACGCTTTTGTCCTGTGCGACGTCTGGAACTACACCA
  AGTTCGACAAGCCTCGCGCGGAAACTTCGGAGGCGGTGAGTGTCACCTTGCAGCACGCGGCCCTCTCCAT
  GTTTGTCACCAGCTTCACCACTGCCGCTGCCTTCTACGCTAACTACGTCAGCAACATCACGGCCATCAGG
  TGCTTCGGGGTGTACGCGGGGACCGCCATATTGGTGAATTACGTCTTGATGGTCACCTGGCTCCCGGCTG
  TGATTGTTCTGCACGAGCGGTACCTCCTCAATATATTCACCTGCTTCAGGAAGCCCCAGCCACAAGCCTA
  CGACAAGAGCTGCTGGGCAGTGCTCTGTCAGAAGTGCCGCAGGGTGCTCTTTGCCGTCTCAGAGGCATCG
  CGGATTTTCTTTGAAAAGGTGCTGCCGTGCATAGTCATCAAGTTTCGCTACCTTTGGCTCATCTGGTTCC
  TCGCCCTGACTGTGGGCGGGGCCTACATCGTGTGCGTAAACCCAAAGATGAAACTGCCGTCGCTGGAGCT
  GTCTGAGTTCCAGGTGTTCCGGTCCTCTCATCCTTTCGAGCGCTATGATGCCGAGTTCAAGAAGCTCTTC
  ATGTTTGAGCGGGTTCACCACGGAGAGGAGCTGCATATGCCCATCACGGTGATCTGGGGCGTGTCCCCAG
  AAGACAGCGGCGACCCTCTGAACCCCAAGAGCAAAGGGGAGCTGACACTAGATAGCACGTTCAACATTGC
  TAGCCCAGCCTCCCAGGCCTGGATTTTACACTTCTGTCAGAAGCTGAGGAATCAGACCTTCTTCCACCAG
  ACGGAGCAGCAGGACTTCACCAGCTGTTTCATCGAGACCTTCAAACAGTGGATGGAGAACCAGGACTGTG
  ACGAGCCTGCCCTGTATCCCTGCTGCAGCCACTGCAGCTTCCCCTATAAGCAAGAGGTTTTCGAGCTGTG
  CATCAAGAAGGCCATCATGGAGCTGGACAGAAGCACGGGATATCATCTGAATAACAAGACCCCCGGGCCA
  AGGTTCGACATCAACGACACCATCAGGGCGGTGGTGCTGGAGTTCCAGAGCACCTTCCTCTTCACCCTGG
  CCTACGAGAAGATGCAGCAGTTCTACAAGGAGGTGGACTCGTGGATTTCCCACGAGCTGAGTTCGGCCCC
  CGAGGGCCTCAGCCGCGGCTGGTTTGTCAGCAACCTGGAGTTCTACGACCTGCAGGACAGCCTTTCCGAC
  GGCACCCTCATCGCCATGGGGCTCTCGGTGGCCGTTGCCTTCAGCGTGATGCTGCTGACCACGTGGAATA
  TCATCATAAGCCTGTACGCCATCGTCTCCATAGCTGGAACCATATTTGTCACAGTCGGGTCCCTGGTCCT
  GCTGGGCTGGGAGCTGAACGTGCTGGAGTCCGTCACCATCTCGGTCGCAGTCGGCCTGTCTGTAGACTTT
  GCCGTCCACTACGGGGTGGCGTACCGCTTGGCTCCAGATCCCGACCGAGAAGGCAAGGTCATCTTCTCTC
  TGAGCCGTATGGGCTCTGCGATCGCCATGGCTGCACTGACCACCTTCGTGGCGGGGGCCATGATGATGCC
  CTCCACGGTCCTGGCTTACACGCAGCTGGGCACGTTCATGATGCTCGTCATGTGCGTGAGCTGGGCCTTC
  GCCACCTTCTTCTTCCAGTGCCTGTGTCGGTGCCTGGGGCCACAAGGCACCTGCGGCCAGATCCCTTTCC
  CTACAAAACTCCAGTGCAGTCCCTTTTCCCACACCTTGTCTGCAAGGCCTGGGGACAGGGGACCAAGCAA
  AACACATGCCGCCAGTGCGTACAGCGTAGATGCCAGGGGCCAGAAATCCCAACTGGAGCATGAGTTTTAC
  GAGTTACAGCCCCTGGCATCTCACAGCTGCACTTCCTCGGAGAAGACTACGTACGAAGAGCCACACACCT
  GCTCCGAATTTTTCAACGGCCAAGCAAAGAATTTAAGGATGCCTGTGCCTGCAGCCTACAGCAGCGAACT
  CACCAAAAGCCCCAGCAGTGAGCCAGGCTCAGCCTTGCTGCAGTCCTGTCTGGAGCAGGACACCGTGTGC
  CATTTCTCTCTCAATCCGAGATGTAACTGCCGAGACGCCTACACACACTTACAGTACGGATTACCAGAAA
  TCCACTGCCAGCAGATGGGTGACTCCTTATGCCACAAGTGTGCCTCCACTGCAGGCGGCTTTGTCCAGAT
  TCAGAGTTCGGTGGCACCTCTGAAGGCCTCACACCAAGCCGCCGAGGGCCTTCTGCACCCTGCCCAGCAC
  ATGCTGCCCCCAGGGATGCAGAATTCTCGGCCTAGGAATTTCTTTCTCCACTCAGTGCAGCACTTTCAGG
  CCCAAGAAAACCTGGGTCGCACCAGCACACACAGCACGGATGAGCGTCTTCCCAGGACAGCAGAGCTGTC
  ACCGCCACCGTCTGACAGCAGGAGCACTGAGTCTTTCCAAAGAGCTTGTTGCCATCCTGAGAATAACCAA
  AGGAGACTCTGCAAAAGTAGAGACCCAGGGGACACAGAGGGCAGTGGAGGGACAAAATCCAAGGTCTCTG
  GTTTACCAAACCAGACTGACAAGGAGGAGAAGCAAGTGGAGCCAAGCCTGCTGCAGACCGATGAAACTGT
  GAACTCAGAACATTTAAATCATAACGAATCAAACTTTACATTCAGCCATTTACCAGGGGAGGCTGGCTGC
  AGGTCCTGCCCAAACAGTCCACAGAGTTGTAGAAGCATCATGAGATCGAAGTGCGGGACTGAGGACTGCC
  AGACGCCAAACCTTGAAGCCAATGTGCCTGCTGTACCAACACACTCAGACCTGTCTGGCGAGAGTCTGTT
  AATAAAAACACTATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: AscI-MluI
• ACCN: NM_001278218
• Insert Size: 4566 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001278218.1, NP_001265147.1
• RefSeq Size: 4942 bp
• RefSeq ORF: 4566 bp
• Locus ID: 68897
• UniProt ID: Q3TDN0
• Cytogenetics: 1
• Gene Summary: Functions in hedgehog (Hh) signaling. Regulates the release and extracellular accumulation of cholesterol-modified hedgehog proteins and is hence required for effective production of the Hh signal. Synergizes with SCUBE2 to cause an increase in SHH secretion (PubMed:22902404).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1, 2, 3, and 4 encode the same protein.