Fmn1 (NM_001285458) Mouse Untagged Clone

CAT#: MC229563

Fmn1 (untagged) - Mouse formin 1 (Fmn1), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Fmn1
• Synonyms: Fmn; formin-1; ld
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229563 representing NM_001285458
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGGCACTCACTGCACCCTCCAGCTGCATAACCCCATTGCCGAACTCTGCTATATCAGCTTCTATC
  TTCCAAAAGGGGAAGTCAGAGGATTTTCATACAAGGGCACTGTAACTCTAGACAGATCCAATAACGCTTT
  CCATAACTGCTACCAAGTCAGGGAGGGGCCAGACATCACCAGCCTCAGCCAGCAGCCAAACGAACATCCA
  GGCGACATATTTTTCAAACAGACTCCCACAAAAAACATCCTGACAGAGCTATACAAGCTCACAGCAGAGA
  AGGAGAGGCTGCTGGACAGTCTGCTGAGGTCAGACAACATCCTCGGTGTTTCAATGGGGAGCCAGGAGGG
  AAAGTTGCAGGAGCTGTCAGTAATCCTTGCCACTGGGGATGAGTATTTCCAGAGTGCTGGCAACTGGCGC
  AGAGAACTCCCTGTGAGCTCTCTCATTAGGAGGAGCACCCAAGAGAACAAAAAGCCCCGGAGGTCTGGCA
  GGAGGAGAGAGAGCCCGGAGGAGCTCCGGCAGAAGAGAACCAGGAGGAAAGGGCGTGGCTGCCAGGAGTC
  AGCATTTCAGATGGGGAAGGACCAGGTCTGTTCCAGTAGCTCCCTTTCTTTTCGAGCTCGGCCTAATCTC
  CGGCTCCTAGAAGAAAGAGGAAATTTAGTTCCTCGGGGAACGCTTACCTCTTCGCTACGGAGAAGAGAGA
  GCTGCCCAGCCAACATCCTCAGGACACCGGATGCAGACCTGGCCTTCGGAAACTCTGGGAGAACCTCAGA
  GGACACTGATCTTGAAGGACCTCTGTCCCCTGACAGCAGCCCCACTGAGGTAGGAGATGCTGATGTGGGA
  GGGCAGCTCAAGAGTTCTCACCAGCAGGAGCCCCCACAACCAAATGTGTCTGAAAGCCATGGGAAACATG
  CAGGGGCAGAGAGGTGGAGCAGCAGGACTCGGAAGTCAAAGTCATTGGAGAGGACTTGCAGTAAGAAACC
  TGTTTCCAAAGTGGTGGCCAAGATTCAGGAACCATCTGCCCCAGTAAAACGAATAGTTAGGGCGCATCAT
  GACGGCAAGGGAAGGGTTGCCTATGGCCCAGAGACCCAAACTGAGTTTATCCCAAAAGCTGACTTCCTCA
  CCCTCCCAGGAGGTGAAACTGAGACTCATAGTTCCGGGAGGCTGGAGGAGGAGCAGCCAGGGATCAAGTC
  ATTGCGGTCCTCAGCCCCAGAAAGAGCCTCCATTACCAAAGAACCAGCCAGCACCGAGGCCGCTGTGAAC
  AAGGTTCTCCGCAAGGTGATAGAGAGTGAGAAGTTAGACGAAGCTACAGAGGGGAAAAGGTTGGGCTTCT
  CTCTCAACACAAGGGCCACCCACACTTTCCCAGAAACTCGAAGCCAAAGGAAGGCTGGGCTACCGCAGAG
  TGGCCATAAATTCTTGCTTCTGGATCTGCCGCACACGGTAGGTCCTGATTCCCCACAACCCAAATGCGAT
  GAGAAGAAGCCAACCCCCCAGGTTCCAACAGCTCTTGGCATGGTATTTAATAATTCATCGCCTCAGTCCA
  GCGCACACAAACGGCTGTCACCTGTTCCCTCGCCTCTGTCTCCAAGGTGTCCCAGTCCACAGCAGCATCA
  TAGGATCCTTCTGCTCCCACCACTGCCTAGTGAGGGAGAAGTTGTTTTTAATGAGTACCCTAGTAGAAAG
  AACGACGTCTCCTCTGGGTTCCCCTCTGCTGACACCTTGGAGCCATCATCTACTACGAAGGTCACGGAGA
  CCAAAGGAGCCAGCCCGACTTCCCTCAGAGCAAGCCAAACTTGGCTGGTGTCTGAGGAAGCTTCCGAAAA
  AGGCTTGGGGCCAGAGAAGATCACAGCTCCACCCCAGCACCAGTTGCCACCAGAATACCAGGCTGCTATC
  CTACATCTGAAGAGGGAGCACAAAGAAGAAATCGAAACCCTGCAGGCTCAGTTTGAACTCAAGACATTTC
  ACATCCGGGGTGAGCACGCATTAGTAACAGCAAGACTTGAAGAAGCCATTGAAAATCTCAAGCAACAGTT
  AGGAAAGCGCCGGGAAGGATGTGAAGAGATGAGAGATGTGTGCATTTCCACAGATGATGACTGCTCTCCA
  AAGGCTTTCAGAAATGTGTGCATCCAGACAGACAGAGAGACCTTCCTCAAGCCCTGTGATGCTGAAAGCA
  AAGCAACCAGAAGCAGCCAGATAGTACCCAAGAAGCTGACTATCTCCTTAACCCAGCTCTCTCCCTCAAA
  AGACAGCAAAGACATCCACGCCCCATTCCAGACAAGAGAAGGCACCTCCTCATCTAGTCAGCATAAGATA
  TCCCCTCCAGCTCCCCCCACGCCACCACCACTCCCTCCACCTCTCATTCCTCCACCTCCGCCCCTCCCAC
  CTGGACTTGGACCTTTGCCTCCAGCACCACCCATACCACCTGTGTGTCCTGTGTCGCCACCGCCACCTCC
  TCCGCCTCCTCCTCCAACTCCCGTGCCTCCAAGTGATGGACCACCACCACCACCACCTCCTCCACCACCA
  CTTCCCAATGTCCTAGCTCTTCCTAACAGTGGAGGTCCTCCTCCTCCTCCACCTCCTCCTCCGCCAGGAC
  TTGCACCCCCACCTCCTCCTGGACTGTCCTTTGGACTCAGCTCTTCTTCCAGCCAGTATCCTCGTAAACC
  AGCCATTGAGCCCAGCTGTCCTATGAAACCTTTGTATTGGACCAGAATACAAATAAATGATAAAAGCCAA
  GACGCCGCACCAACTTTATGGGACTCCTTAGAAGAGCCTCATATTAGGGACACAAGTGAATTTGAATATT
  TATTCTCCAAGGACACAACTCAACAGAAGAAAAAACCCCTGTCAGAGGCCTACGAAAAGAAGAACAAAGT
  CAAAAAGATCATCAAGTTATTGGATGGAAAGCGATCTCAAACTGTGGGAATCTTGATATCTAGTTTACAT
  CTAGAAATGAAAGATATTCAACAGGCCATATTTACTGTGGATGACTCCGTGGTTGACCTGGAGACCTTAG
  CAGCCTTATATGAAAATCGAGCCCAGGAGGATGAACTGACTAAAATAAGAAAGTACTATGAGACATCCAA
  AGAAGAAGACTTGAAACTGCTGGACAAACCTGAACAATTTTTGCATGAGTTAGCCCAGATTCCCAATTTT
  GCCGAACGTGCCCAGTGCATAATCTTCAGGGCTGTATTTTCTGAGGGTATCACATCCTTACATCGAAAAG
  TAGAGATTGTCACACGGGCCTCGAAGGGCTTGTTGCACATGAAGAGTGTGAAGGATATCTTAGCTCTCAT
  TCTGGCTTTTGGAAACTACATGAATGGAGGAAACAGGACGCGAGGGCAAGCAGACGGATATAGTTTAGAA
  ATTCTGCCCAAACTCAAAGACGTCAAAAGTCGGGACAATGGGATGAATCTGGTGGACTATGTTGTGAAGT
  ACTACCTGCGATACTATGATCAGGAAGCTGGAACAGACAAGAGTGTTTTCCCGCTGCCTGAACCACAGGA
  TTTCTTCCTGGCCTCTCAAGTCAAGTTTGAAGACCTCCTAAAGGATTTGAGGAAGCTGAAGCGTCAACTA
  GAAGCAAGTGAGCAACAGATGAAGCTGGTGTGCAAGGAGTCCCCAAGGGAGTACCTGCAGCCTTTCAAGG
  ACAAACTGGAGGAGTTCTTCAAGAAAGCCAAAAAAGAGCACAAGATGGAAGAAAGTCACTTGGAGAATGC
  ACAGAAAAGTTTTGAAACAACAGTGGGATATTTTGGAATGAAGCCAAAGACTGGAGAGAAGGAGGTCACC
  CCCAGCTATGTGTTTATGGTGTGGTTTGAGTTCTGCAGTGACTTCAAGACCATTTGGAAGCGGGAGAGTA
  AGAACATATCTAAAGAAAGATTGAAAATGGCTCAGGCATCCGTCAGCAAACTGACATCAGAGAAGAAAGT
  GGAGACAAAGAAAATCAATCCCACCGCTAGTCTGAAAGAAAGACTGCGTCAGAAGGAAGCCAGCGTGGCC
  ACCAACTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001285458
• Insert Size: 3999 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001285458.1, NP_001272387.1
• RefSeq Size: 11523 bp
• RefSeq ORF: 3999 bp
• Locus ID: 14260
• Cytogenetics: 2 57.3 cM
• Gene Summary: Plays a role in the formation of adherens junction and the polymerization of linear actin cables.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) lacks three alternate in-frame exons compared to variant 1. The resulting protein (isoform 3) is shorter compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.