Ppfia2 (NM_001205341) Mouse Untagged Clone

CAT#: MC229522

Ppfia2 (untagged) - Mouse protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 2 (Ppfia2), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ppfia2
• Synonyms: 5330438O12; B230207K17Rik; E130120L08Rik; mKIAA4112
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229522 representing NM_001205341
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGTGTGAAGTGATGCCCACGATTAACGAGGACACCCCAATGAGCCAACGGGGTTCCCAAAGCAGTG
  GCTCCGACTCGGACTCCCATTTTGAGCAACTCATGGTCAATATGCTGGATGAAAGGGACCGCCTACTGGA
  CACACTCCGAGAGACCCAGGAGAGCCTCTCACTTGCCCAGCAAAGGCTCCAGGATGTCATCTATGACAGA
  GATTCACTCCAGAGACAGCTGAATTCAGCCCTACCTCAGGACATCGAATCCCTAACAGGAGGGCTGACTG
  GTTCTAAGGGGGCTGATCCACCGGAGTTTGCTGCACTGACTAAAGAGTTGAATGCTTGCCGTGAACAACT
  TCTAGAAAAGGAAGAAGAAATCTCAGAGCTGAAAGCCGAAAGAAACAACACAAGACTGTTACTGGAACAC
  TTGGAGTGCCTTGTGTCCCGACATGAACGGTCACTAAGAATGACGGTGGTCAAGCGGCAAGCCCAGTCTC
  CCTCTGGAGTGTCCAGTGAAGTGGAGGTTCTCAAGGCACTGAAATCTTTGTTTGAACACCACAAAGCCTT
  GGATGAAAAGGTAAGGGAGCGACTGAGGGTTTCTTTAGAAAGAGTCTCTGCACTGGAAGAGGAACTAGCT
  GCAGCTAATCAGGAGATTGTTGCCTTGCGTGAGCAAAATGTTCATATTCAAAGGAAAATGGTATCAAGTG
  AGGGGTCCACGGAGTCAGAGCATCTTGAAGGAATGGAAGCAGGCCAGAAAGTTCATGAAAAGCGTCTATC
  CAATGGCTCCATAGATTCGACAGATGACACCAGCCAAATCGTCGAGCTGCAAGAATTGCTTGAGAAGCAA
  AACTATGAAATGGCCCAAATGAAAGAACGCTTAACAGCTCTCTCTTCCCGGGTGGGAGAGGTGGAACAAG
  AAGCAGAGACAGCAAGAAAGGACCTCATTAAAACAGAAGAAATGAACACAAAATATCAAAGGGACATCCG
  AGAGGCCATGGCTCAGAAGGAAGATATGGAAGAAAGAATCACAACTCTGGAGAAGCGTTACCTGAGTGCT
  CAGAGAGAATCTACCTCCATTCATGATATGAATGATAAACTAGAAAATGAATTGGCGAACAAGGAAGCCA
  TCTTGCGGCAGATGGAAGAAAAAAACAGGCAATTGCAAGAGCGTCTTGAACTGGCTGAGCAAAAGTTACA
  GCAGACCATGAGAAAAGCTGAAACTTTACCTGAAGTAGAGGCTGAACTTGCTCAGAGAATTGCAGCCCTA
  ACAAAGGCTGAAGAGAGGCATGGAAATATTGAAGAACGTATGAGACACCTCGAGGGCCAACTTGAAGAGA
  AGAATCAAGAACTTCAAAGAGCTAGACAAAGGGAGAAAATGAATGAAGAGCATAATAAGAGATTATCGGA
  TACGGTGGACAGGCTTCTGACAGAATCCAATGAGCGCCTGCAACTACACCTGAAGGAAAGGATGGCTGCT
  TTGGAAGAGAAGAATGTTTTGATTCAAGAATCAGAAAACTTCAGAAAAAATCTTGAAGAGTCCTTACATG
  ATAAGGAAAGATTAGCAGAAGAAATCGAGAAGCTGAGGTCTGAACTTGATCAAATGAAAATGAGAACAGG
  TTCTCTAATTGAACCCACTATATCAAGAACTCATATAGACACCTCCACTGAGTTGCGGTATTCAGTTGGG
  TCCCTTGTAGACAGCCAGTCTGATTACAGAACCACTAAAGTAATACGAAGACCAAGGAGAGGCCGCATGG
  GTGTGCGAAGAGATGAACCAAAGGTGAAATCCCTTGGAGATCATGAGTGGAACAGAACTCAGCAAATTGG
  AGTACTAGGAAGCCACCCTTTCGAAAGTGACACTGAAATGTCTGATATTGATGATGATGACAGAGAAACA
  ATTTTTAGCTCAATGGATCTTCTCTCTCCAAGTGGCCATTCTGATGCCCAGACTCTAGCTATGATGCTTC
  AAGAACAATTGGATGCAATCAATAAAGAAATAAGACTAATTCAAGAAGAAAAGGAATCGACAGAATTGCG
  TGCTGAAGAAATTGAGAATAGAGTGGCTAGTGTAAGCTTAGAAGGGCTGAATTTGGCGAGGGTCCATCCA
  GGTACCTCCATCACTGCCTCTGTTACAGCTTCATCACTGGCCAGTTCATCTCCCCCAAGTGGACACTCAA
  CGCCAAAGCTTACACCAAGAAGCCCTGCCAGGGAAATGGACCGCATGGGAGTGATGACCCTGCCAAGTGA
  TCTAAGGAAACATCGGAGAAAGATTGCAGTGGTGGAAGAAGATGGACGGGAGGATAAAGCCACAATTAAA
  TGCGAGACTTCTCCTCCCCCAACACCCAGAGCCGTCAGAATGACTCACACCTTACCATCTTCCTACCACA
  ATGATGCCCGGAGCAGTTTATCTGCCTCTCTTGAGCCAGACAGTCTTGGGCTTGGCAGTGCCAATAGCAG
  CCAAGATTCTCTCCACAAAGCCCCCAAGAAGAAAGGAATCAAGTCTTCAATTGGGCGCTTATTTGGGAAA
  AAAGAGAAGGCTCGACTCGGGCAGCTTCGAGGCTTCATGGAGACAGAAGCTGCAGCACAGGAATCCCTGG
  GCTTAGGCAAACTTGGAACTCAAGCTGAAAAGGACAGAAGGCTGAAGAAAAAGCATGAACTTCTTGAAGA
  AGCTCGTAGAAAAGGATTACCTTTTGCCCAGTGGGATGGACCCACCGTGGTTGCGTGGCTGGAGCTCTGG
  CTGGGAATGCCAGCTTGGTACGTGGCAGCATGCAGAGCCAACGTGAAAAGTGGTGCCATCATGTCAGCGT
  TATCGGACACTGAAATCCAAAGGGAGATTGGAATCAGCAACCCTCTGCACCGCTTAAAGCTCAGGCTAGC
  TATCCAAGAGATGGTTTCTCTCACCAGCCCTTCAGCGCCTCCGACATCGCGTACTCCTTCAGGCAATGTG
  TGGGTGACCCATGAAGAAATGGAAAATCTGACAGCTCCAGCAAAAACGAAAGAATCTGAGGAAGGAAGCT
  GGGCCCAGTGTCCGGTTTTTCTACAGACCCTGGCTTATGGAGATATGAACCACGAGTGGATTGGAAATGA
  ATGGCTTCCCAGCCTGGGGTTACCTCAATACAGAAGTTACTTTATGGAATGCTTGGTTGATGCAAGAATG
  TTAGACCACCTCACAAAAAAGGATCTTCGTGTCCATTTAAAAATGGTGGATAGTTTCCATCGAACAAGTT
  TACAGTATGGAATTATGTGCTTGAAAAGATTGAATTATGACAGAAAAGAACTAGAGAGAAGACGAGAAGC
  AAGTCAACATGAAATAAAAGATGTTTTAGTGTGGAGCAATGATCGAGTTATTCGCTGGATACAAGCAATT
  GGACTTCGGGAATATGCAAACAACATTCTTGAGAGTGGCGTGCATGGCTCACTTATAGCCCTGGATGAGA
  ACTTTGACTACAGCAGCTTGGCTCTATTGTTGCAGATTCCAACACAGAACACCCAGGCAAGGCAGATTCT
  TGAACGTGAGTACAACAACCTCTTGGCCCTGGGAACTGAGCGGAGACTGGATGAAAGTGACGACAAGAAT
  TTCAGACGGGGATCAACCTGGCGAAGGCAGTTCCCTCCTCGTGAAGTTCATGGAATCAGCATGATGCCTG
  GGTCCTCAGAAACATTACCAGCTGGATTCAGGTTGACCACAACGTCTGGGCAGTCCAGGAAAATGACGAC
  AGACGTTGCTTCATCAAGACTGCAGAGGTTAGACAACTCCACTGTTCGCACATACTCATGTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001205341
• Insert Size: 3774 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001205341.1, NP_001192270.1
• RefSeq Size: 6454 bp
• RefSeq ORF: 3774 bp
• Locus ID: 327814
• Cytogenetics: 10 D1
• Gene Summary: Alters PTPRF cellular localization and induces PTPRF clustering. May regulate the disassembly of focal adhesions. May localize receptor-like tyrosine phosphatases type 2A at specific sites on the plasma membrane, possibly regulating their interaction with the extracellular environment and their association with substrates (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.