Fhdc1 (NM_001205355) Mouse Untagged Clone

CAT#: MC229415

Fhdc1 (untagged) - Mouse FH2 domain containing 1 (Fhdc1), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Fhdc1
• Synonyms: 6330505N24Rik; Gm126
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229415 representing NM_001205355
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCATGTTATGAATTGTGTCTCCCTGGCCAGCGATAAAGAAAATGGGACTCTTGCCACAGCAGCTGCAT
  TCATGACTGGGCAGACGTCACCCTCTGCATCTCCTCCACCTCCTCCTCCACCGCCTCCACCTCCACCTTG
  TCCACATTCAGGGGAGGGCTTTTCCCCCTCCCCTCCTCCTCCTCTGCCACCTCCACTTCCTGGGGGACCT
  CCCATACCCCCTCCTCCTCCTCCAGGACTCCCCTCAGTTTCTTACCTGAATGGCTACAGCTCCCTGGGTA
  AGAAGAAACGAATGCGGAGCTTTTTCTGGAAAACCATTCCAGAGGAGCAAGTTCGAGGCAAGACCAACAT
  CTGGACCTTAGCAGCTAAGCAGCAGCATCAGTACCAGATCGACAAGAAGACCATTGAGGAGCTTTTTGGG
  CAGCAAGAAGACACCTCCAAGGCTTCTCTTCCCAAGAGAGGAGGAGCTTTGAATTCATCCTTCAGAGATG
  CCCGGGAAGAGGTTACCGTCTTGGATGCAAAGCGAAGTATGAACATTGGCATATTTCTTAAGCAGTTTAA
  AAAGTCTCCTCAGTCCATCGTAGAGGATATCTATCAAGGAAAGAGCGAGCATTACGGATCAGAGACGCTT
  CGAGAAATCCTTAAGCTTTTGCCGGAGTCAGAAGAGGTAAAGAAATTAAAGGCATTTAATGGCGATGTAT
  CCAAGCTCTCGCTGGCAGATTCCTTTCTGCACTGCTTGATTCAGGTGCCAAACTATTCACTTCGGATTGA
  AGCCATGGTGCTAAAAAAGGAGTTTTTGCCATCTTGTTCCTCTCTGTTCAAAGACATAAGGACTCTGAGA
  GCAGCTACAAAAGAGCTGATGTTATGTGAGGAGCTACATTCAATATTACACTTGGTGCTCCAGGCTGGGA
  ATATCATGAATGCCGGAGGGTATGCCGGCAATGCCGTAGGATTTAAACTGTCTTCTCTACTCAAATTGGC
  GGACACGAAGGCAAACAAACCTGGAATGAATCTTCTGCATTTTGTAGCTCAGGAAGCCCAGAAGCAAGAT
  GCCATCCTTCTAAACTTCTCTGAGAAGTTGCAGCACGTGCAAGAGACATCGAGATTATCTCTGGATATCA
  CCGAGGCAGAGCTGCACTCTCTCTTTGTCAGAACGAAATCGCTGCAGGAAAACATCCAGCTTGACCAGGA
  GCTGTGCCAGCAGATGGAAGACTTCCTCCAGTTTGCTGTGGAGAAGTTGGCGGAGCTGGAACTCTGGAAA
  CGGGAGCTGCAGGGTGAAGCTCACACCCTTATAGACTTTTTCTGCGAAGACAAAGAAACCATGAAACTGG
  ATGAATGCTTCCAGATATTCAGAGACTTCTGTACCAGATTCAACAAAGCAGTTAAGGACAATCATGACCG
  GGAGGAACAGGAGCGGAAGCAGCTACAGAGGCAGAAGGAGATGGAACAGAAGCGGTATTCCTGGTCAACT
  GGGGAACTCGGGAGCTTTGGCAGGAGCAGCAGTGAGAATGATGTGCAGATGCTAGCTAAGACAGGCACAG
  AAGACCTGCCCTCCTTCCTGAAGCCCAGGCCCAACAGCCCCTCCTACCGGCCCCCCAACACACGTCGCTC
  CCGCCTCTCCCTGGGCATCTCTGCGGACCGGGAGCTTTTGACCTTCCTGGAGAGTGCCACCAGCAGTCCA
  GAGGACCCCAACAAATTCAACAGTTTGCCCCGGAGCAGTCCCCGACAAGCTCGGCCCACCATCGCCTGGA
  TGGAACCCAGAGAACAACAGAGCCATGGCCCTAACTTTACACATGAACCTCAGGCCTCAAAGATCCAGGA
  GAAAGCCCCTCCCCCTGCTTGGCAAAATCAGCTTCCGACAACCTGGCGGGAGGAGCCTGCTTCTCCCCTC
  CCACTGGCTGGGCGGAGCAGGCCGAGCCTCCGAAAAAGGAACAGTGAACCTGTGGGCTTGGGTCCCACAC
  AAAGCCCTCCACTCTTACCATTGGATCTGGGTGTTAGGGAGCATGAGTTGGTCACAGGGCTGACCCAGTT
  TGACCTCCAGAGTCCCAAGAGCCTGGAGGAAGGATCCCAACTGACTCTGAATGACTTCTGTCCCACAAAG
  CTGCCATCTCCAGGGGATAGGAGCTCACAGCCCTTTGCTGCTGGCGGTGACAGTCTCCCACCCAAGGACA
  CAGACACCCAGGAGGTTCTCAGCCCAGCTGGGGAGGATGACAGGACCATCTCTGATGAACCCAGCAGTGA
  GGCTCTCGTGTCTGTGGTTGTCACCGACACTGAAGATAAGGATGCTGGACCTCTGTTATATGTCTCAGAT
  ACCACAGACTGCTCACTGACTCTGGACTGCTCCGAGGGAATGGACTCCAGAGCCGGGGGAGACAAGCAAG
  AGGAAGAGAAGGAAGGGGATGGCTCAGTGTCCTCTGGGGCTGGAGAGGCAGGAAGCAGCCAGGTTTCCTC
  CAACTCTGTTTCCAGCCCCCCTGGGGAGGTTCCTGCTCCAAAATCCAGTAAGAGTGAGCTGAGCTGCCAA
  GGTGGCCTTCCGAAGGACAGACCCAGCAGAGGGAAGGATGCTATAGCACCAAAGAGAAATTCCTTCAAAG
  AGGCTTCAGTAGGGGCTTCAAAGCCTGTCAGTGCACGGCGCAGCCAAGGGGTAACAACCAAGCCTGTGCG
  AACCTTGAACTCCTCCGAGAACGAACATATGCGCAAAGTGGTCCCTATCTCCAAGTCCAGCAGGGGAGCC
  GGGCCCTGGAAGCGGCCGGAACCGACACCCAAGGCTACCCCTCGGGAGACACCCAGCAGCACAGACACAC
  CACTATCACGAAGGAGCTCAGTGAGAGGAACTTCGGACACTTCGCCCAGGAGGCCCCAGGTGAGTGGATC
  TGGGGCAGAGGAGCCCAGGCTGCCCCGCTCGAGTGGCAGCATCAGTGGACGGCCAGGGAAGGACGCCCCG
  CTGCAGCCCAGAGCCTCGTTCAGGAAGCCTAGCGCCAAGCCCCTCAGAAACATCCCCAGGCAGAAACCTG
  AAGAAAACAAGGTCAGCAGCCCCAACTCTCCTGACCCCGAGAGTCCCAAGGAAGAGCCCAAGGCTCCTCA
  GGCTACGGGTGTCTCTCGAGCCCTTCCCCCCATCCCAAGTTTTGCTCGAAATACGGTGGCCTCGTCATCT
  CGGAGCCTGCGAACGGATGCTCCACCCGCGGCCAGAACCACTGGCCTCACCAGGACAGTCTCCCAGCGGC
  AGCTTAGGGTCAAAGGTGGCTCCGAGGATTCTGCCTCCAAGGACATTGGCACTTTGAAGAGGGCCAGCAG
  TGCTCGGGCCTCCAAGAAGTGTCCTGAATCTGCTGGGGGTTCAAGTGCCAATGTAGAAACCTCACTGAAG
  GGCAGAGGGACGACGGAAAGATCCTCTCTCAGACTGAAGGACTCGGGGCAGGCCACCCTCGGGAGGATCC
  TCCGTCCCCTGCAGAAATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001205355
• Insert Size: 3450 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001205355.1, NP_001192284.1
• RefSeq Size: 6001 bp
• RefSeq ORF: 3450 bp
• Locus ID: 229474
• UniProt ID: Q3ULZ2
• Cytogenetics: 3 F1
• Gene Summary: Microtubule-associated formin which regulates both actin and microtubule dynamics. Induces microtubule acetylation and stabilization and actin stress fiber formation (PubMed:18815276). Regulates Golgi ribbon formation (PubMed:26564798). Required for normal cilia assembly. Early in cilia assembly, may assist in the maturation and positioning of the centrosome/basal body, and once cilia assembly has initiated, may also promote cilia elongation by inhibiting disassembly (PubMed:29742020).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.