Xpo1 (NM_134014) Mouse Untagged Clone

CAT#: MC229335

Xpo1 (untagged) - Mouse exportin 1, CRM1 homolog (yeast) (Xpo1), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Xpo1
• Synonyms: AA420417; Crm1; Exp1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229335 representing NM_134014
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCAGCAATTATGACAATGTTAGCAGACCATGCAGCTCGTCAGCTGCTTGATTTCAGCCAAAAACTGG
  ATATCAACCTTTTAGACAACGTGGTGAATTGCTTATACCATGGAGAAGGAGCCCAGCAAAGAATGGCTCA
  AGAAGTACTGACACATTTAAAGGAGCATCCTGATGCTTGGACACGGGTCGACACAATATTGGAGTTTTCG
  CAGAACATGAACACGAAATACTATGGACTACAGATTTTGGAAAATGTGATCAAAACAAGGTGGAAGATCC
  TTCCAAGGAACCAGTGTGAAGGAATAAAAAAATATGTTGTTGGTCTCATTATCAAGACATCCTCTGACCC
  AACTTGTGTAGAGAAAGAAAAGGTGTATATTGGGAAATTGAATATGATTCTTGTTCAGATACTTAAGCAA
  GAGTGGCCTAAGCATTGGCCAACTTTTATCAGTGACATTGTTGGAGCGAGTAGGACCAGTGAAAGTCTAT
  GTCAAAATAATATGGTGATTCTTAAACTCTTGAGTGAAGAAGTGTTTGATTTCTCTAGTGGACAGATAAC
  CCAAGTTAAAGCAAAGCATTTAAAAGACAGCATGTGCAATGAGTTTTCACAAATATTTCAGCTTTGTCAG
  TTTGTGATGGAAAATTCCCAAAATGCTCCCCTTGTTCATGCTACATTAGAAACACTGCTGAGATTTCTGA
  ACTGGATTCCACTGGGATACATCTTTGAGACCAAGTTAATCAGCACATTAATTTACAAGTTCCTGAATGT
  TCCAATGTTCCGAAATGTGTCTCTAAAGTGCCTCACTGAGATTGCTGGTGTGAGTGTAAGCCAATATGAG
  GAACAATTTGAAACTCTGTTTACACTTACAATGATGCAGCTAAAACAGATGCTTCCTTTAAACACTAATA
  TCCGACTTGCATACTCGAATGGGAAAGATGATGAACAGAACTTTATTCAAAATCTCAGCCTGTTTCTCTG
  TACCTTTCTAAAAGAGCACGGTCAACTTCTAGAAAAACGACTGAATCTCAGGGAAGCACTCATGGAGGCT
  CTTCATTATATGTTGTTGGTGTCTGAAGTGGAAGAAACTGAAATTTTTAAAATCTGCCTTGAGTACTGGA
  ATCATTTGGCAGCTGAGCTCTACAGAGAGAGTCCATTTTCTACATCTGCTTCTCCCTTGTTATCTGGAAG
  TCAACACTTTGATATTCCTCCTAGGAGACAGCTGTACTTGACCGTGTTATCAAAGGTCCGTTTATTGATG
  GTTAGTCGCATGGCTAAACCAGAGGAGGTACTGGTTGTAGAAAATGATCAGGGAGAAGTTGTAAGAGAGT
  TCATGAAGGATACTGATTCCATAAATTTATATAAGAATATGAGAGAAACATTAGTTTATCTTACTCATCT
  GGATTATGTAGATACGGAAATAATAATGACTAAGAAACTTCAAAATCAAGTGAATGGTACAGAATGGTCA
  TGGAAGAATTTGAATACGTTGTGTTGGGCAATAGGCTCCATCAGTGGCGCAATGCATGAAGAGGATGAGA
  AGCGGTTTCTTGTTACTGTTATAAAGGATCTTTTAGGATTATGTGAGCAGAAAAGAGGCAAAGATAATAA
  AGCTATTATTGCATCGAATATCATGTACATAGTAGGTCAATACCCAAGATTTTTGAGAGCACACTGGAAA
  TTTTTGAAGACAGTAGTAAACAAGCTATTCGAATTCATGCATGAGACCCATGATGGAGTCCAGGATATGG
  CTTGTGACACATTTATTAAAATAGCTCAAAAATGCCGTAGGCATTTTGTTCAGGTTCAAGTTGGAGAAGT
  AATGCCGTTCATTGATGAGATTTTAAACAACATAAATACTATAATTTGTGATCTTCAGCCTCAGCAGGTT
  CATACATTTTATGAAGCTGTGGGGTACATGATTGGTGCACAGACAGACCAGACAGTTCAAGAGCATTTGA
  TAGAAAAATACATGCTACTCCCTAATCAAGTGTGGGACAGTATAATTCAGCAGGCTACCAAAAATGTGGA
  TATCCTGAAAGATCCTGAAACAGTCAAGCAGCTGGGTAGCATATTAAAAACAAATGTGAGAGCCTGCAAA
  GCTGTTGGTCATCCCTTTGTAATTCAGCTTGGCAGAATTTATTTAGACATGCTTAATGTATATAAGTGCC
  TCAGTGAAAATATTTCTGCAGCTATTCAAGCTAATGGTGAGATGGTTACAAAGCAACCATTGATTAGAAG
  TATGCGGACAGTAAAAAGGGAAACTTTAAAGTTAATATCTGGTTGGGTGAGCCGTTCCAATGATCCTCAG
  ATGGTAGCTGAAAACTTTGTTCCTCCTTTGTTGGATGCAGTTCTTATTGATTACCAGAGAAATGTACCAG
  CTGCTAGAGAACCAGAAGTGCTTAGTACTATGGCCATTATTGTCAACAAATTAGGTGGACATATAACAGC
  TGAAATACCTCAAATATTCGATGCAGTTTTTGAGTGCACATTGAATATGATAAATAAGGATTTTGAAGAG
  TATCCAGAACACAGAACAAACTTTTTCTTATTACTTCAAGCTGTCAATTCTCACTGTTTCCCAGCATTCC
  TAGCTATTCCACCTGCACAGTTCAAGCTGGTTTTGGATTCTATTATTTGGGCTTTTAAACATACTATGAG
  GAATGTTGCAGATACAGGCTTACAGATACTTTTTACACTTTTACAAAATGTTGCACAAGAGGAAGCTGCC
  GCACAGAGTTTTTATCAAACTTATTTTTGTGATATTCTTCAGCATATTTTCTCTGTTGTAACAGACACTT
  CACATACTGCAGGTCTAACAATGCATGCATCAATTCTTGCATATATGTTTAATTTGGTTGAAGAAGGAAA
  AATAAGTACACCACTAAATCCTGGAAATCCAGTTAACAACCAAATGTTTATTCAAGACTATGTGGCTAAT
  CTCCTTAAATCTGCATTCCCTCATCTACAAGATGCTCAAGTAAAGCTCTTTGTAACAGGACTTTTCAGCT
  TAAATCAGGATATTCCTGCTTTCAAAGAACACCTAAGGGATTTCTTAGTTCAAATAAAGGAGTTTGCAGG
  TGAAGATACATCGGATCTATTTTTGGAAGAAAGAGAAACAGCCCTTCGACAGGCTCAGGAGGAGAAACAT
  AAACTTCAAATGTCTGTACCTGGGATTCTTAATCCACACGAAATTCCAGAAGAAATGTGTGATTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_134014
• Insert Size: 3216 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_134014.3, NP_598775.2
• RefSeq Size: 5144 bp
• RefSeq ORF: 3216 bp
• Locus ID: 103573
• UniProt ID: Q6P5F9
• Cytogenetics: 11 A3.2
• Gene Summary: Mediates the nuclear export of cellular proteins (cargos) bearing a leucine-rich nuclear export signal (NES) and of RNAs. In the nucleus, in association with RANBP3, binds cooperatively to the NES on its target protein and to the GTPase Ran in its active GTP-bound form. Docking of this complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the cargo from the export receptor. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Involved in U3 snoRNA transport from Cajal bodies to nucleoli. Binds to late precursor U3 snoRNA bearing a TMG cap (By similarity).[UniProtKB/Swiss-Prot Function]