Sema6d (NM_001291000) Mouse Untagged Clone

CAT#: MC229333

Sema6d (untagged) - Mouse sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant 7

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Sema6d
• Synonyms: 1110067B02Rik; AA409156; D330011G23; mKIAA1479
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229333 representing NM_001291000
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGTTCCTTCTGCTTTGGTTCTGCGTGCTGTTCCTTCTGGTCTCCAGGTTACGGGCGGTCAGCTTCC
  CAGAAGACGATGAGCCCCTCAACACGGTTGACTATCACTATTCAAGGCAATATCCGGTTTTTAGAGGACG
  CCCTTCAGGCAACGAATCGCAGCACAGGCTGGACTTTCAGCTGATGTTGAAAATTCGAGACACACTTTAT
  ATTGCTGGCAGGGATCAAGTCTATACAGTGAACTTAAATGAAATCCCCCAAACAGAGGTGATACCAAGCA
  AGAAGCTGACGTGGAGGTCCAGACAGCAGGATCGAGAAAATTGTGCTATGAAAGGCAAGCATAAAGATGA
  ATGCCACAACTTCATCAAAGTCTTTGTCCCAAGAAATGATGAGATGGTTTTTGTCTGTGGTACCAATGCT
  TTCAACCCGATGTGCAGATACTATAGGTTGAGAACGTTAGAGTATGATGGGGAAGAAATTAGTGGCCTGG
  CACGATGCCCGTTTGATGCCCGACAAACCAATGTCGCCCTCTTTGCTGATGGAAAACTCTATTCTGCCAC
  AGTGGCTGATTTCCTGGCCAGTGATGCTGTCATTTACAGAAGCATGGGAGATGGATCTGCCCTTCGCACA
  ATAAAATACGATTCCAAGTGGATCAAAGAACCACACTTCCTTCATGCCATAGAATATGGAAACTATGTCT
  ATTTCTTCTTCAGAGAAATCGCCGTGGAACATAATAACTTAGGCAAGGCTGTGTATTCCCGCGTGGCTCG
  CATTTGTAAAAACGACATGGGTGGCTCACAGCGGGTCCTGGAGAAACACTGGACTTCCTTCCTTAAGGCT
  CGGCTGAACTGCTCCGTTCCTGGAGATTCCTTTTTCTACTTCGACGTCCTGCAGTCTATAACAGACATAA
  TCCAAATCAATGGCATCCCCACTGTGGTTGGGGTCTTCACCACACAGCTCAACAGCATTCCTGGTTCTGC
  AGTCTGTGCCTTTAGCATGGACGACATTGAGAAAGTGTTCAAAGGGCGGTTCAAAGAGCAGAAAACCCCA
  GACTCTGTTTGGACAGCAGTTCCCGAAGACAAAGTACCAAAACCAAGGCCTGGCTGTTGTGCCAAACACG
  GCCTCGCAGAAGCTTACAAGACCTCCATCGACTTTCCAGATGACACCCTGGCTTTCATCAAGTCCCACCC
  GCTGATGGACTCTGCCGTCCCACCCATTGCCGATGAGCCCTGGTTCACAAAGACACGGGTCAGGTACAGG
  TTGACAGCCATCGAAGTGGACCGTTCAGCAGGGCCATACCAAAACTACACAGTCATCTTTGTTGGCTCTG
  AAGCTGGCGTGGTACTTAAAGTTTTGGCAAAGACCAGTCCTTTCTCTCTGAATGACAGTGTATTACTCGA
  AGAGATTGAAGCTTATAACCCAGCCAAGTGCAGCGCCGAGAGTGAGGAGGACAGAAAGGTGGTCTCATTA
  CAGCTGGACAAGGATCACCATGCTTTATACGTGGCCTTCTCTAGCTGCGTGGTCCGCATCCCCCTCAGCC
  GCTGTGAGCGCTACGGATCGTGTAAAAAGTCTTGCATTGCATCACGTGACCCGTACTGTGGTTGGTTAAG
  CCAGGGAGTTTGTGAGAGAGTGACCCTAGGGATGCTCCCTGGAGGATATGAGCAGGACACGGAGTACGGC
  AACACAGCCCACCTAGGGGACTGCCACGAAAGTTTGCCTCCTTCAACTACACCAGATTACAAAATATTTG
  GCGGTCCAACATCTGACATGGAGGTATCCTCATCTTCTGTTACCACTGTGGCAAGTAGCCCAGAAATTAC
  ATCTAAAGTGATTGATACCTGGAGACCTAAACTGACGAGCTCCCGGAAATTTGTAGTTCAAGATGACCCA
  AATACTTCTGATTTTACTGATACTATATCAGGTGTACGGTGGGAAGTCCAGTCTGGAGAATCCAATCAGA
  TGGTCCACATGAATGTCCTCATCACCTGCGTGTTTGCCGCTTTTGTCTTGGGCGCGTTCATCGCAGGAGT
  GGCCGTGTACTGCTACCGTGACATGTTCGTTCGGAAGAACAGAAAGATCCATAAAGACGCAGAATCCGCC
  CAGTCGTGCACAGACTCCAGCGGAAGCTTCGCCAAGCTGAACGGCCTCTTTGACAGCCCCGTCAAGGAAT
  ACCAGCAGAACATTGATTCTCCCAAACTCTACAGCAACCTGCTGACCAGTCGGAAGGAACTGCCACCAAA
  CACGGATACAAAGTCCATGGCCGTGGACCACAGAGGCCAGCCTCCCGAGCTGGCTGCTCTCCCCACGCCG
  GAATCCACACCTGTCCTCCACCAGAAGACCCTGCAGGCCATGAAGAGCCACTCTGAGAAGGCCCACAGCC
  ACGGTGCTTCAAGGAAAGAACACCCCCAGTTTTTTCCTTCTAGTCCTCCACCCCATTCCCCATTGAGTCA
  CGGGCATATCCCCAGTGCCATCGTTCTTCCAAACGCCACTCACGACTACAATACATCCTTCTCCAACTCG
  AATGCCCACAAAGCCGAAAAGAAGCTTCAGAGCATGGATCACCCTCTTACGAAGTCATCCAGTAAGCGGG
  AGCACCGGCGGTCTGTGGATTCCAGGAATACTCTCAATGATCTCCTGAAGCATCTAAATGACCCAAACAG
  TAACCCCAAAGCCATCCTGGGAGAGATCCATATGGCTCATCAAACCCTCATGCTGGACCCGGTGGGACCA
  ATGGCTGAGGTCCCACCCAAGGTCCCTAACCGGGAGGCATCTCTATACTCCCCTCCCTCCACACTCCCCA
  GAAATAGTCCAACCAAGAGAGTAGATGTCCCCACCACTCCTGGGGTGCCAATGACTTCTCTGGAAAGACA
  AAGGGGTTATCACAAAAATTCCTCCCAGAGGCACTCTATATCTGCCGTGCCTAAAAACTTAAACTCACCA
  AATGGTGTTTTGTTATCTAGACAGCCGAGTATGAACCGTGGAGGCTATATGCCCACCCCAACAGGGGCGA
  AGGTGGACTATATTCAGGGGACACCGGTGAGTGTTCATCTGCAGCCCTCCCTCTCCAGACAGAGCAGCTA
  TACCAGTAATGGCACCCTCCCCAGGACGGGACTAAAGAGGACACCATCCTTAAAACCTGATGTGCCACCA
  AAGCCTTCCTTTGTTCCGCAAACCACATCTGTCAGACCACTGAACAAGTACACGTACTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001291000
• Insert Size: 3210 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001291000.1, NP_001277929.1
• RefSeq Size: 6509 bp
• RefSeq ORF: 3210 bp
• Locus ID: 214968
• UniProt ID: Q76KF0
• Cytogenetics: 2 F1
• Gene Summary: Shows growth cone collapsing activity on dorsal root ganglion (DRG) neurons in vitro. May be a stop signal for the DRG neurons in their target areas, and possibly also for other neurons. May also be involved in the maintenance and remodeling of neuronal connections (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (7) uses an alternate in-frame splice site and contains two additional in-frame exons in the central coding region, compared to variant 1. The encoded isoform (3) is longer than isoform Sema6D-1.