Ogdh (NM_001252282) Mouse Untagged Clone

CAT#: MC229303

Ogdh (untagged) - Mouse oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide) (Ogdh), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ogdh
• Synonyms: 2210403E04Rik; 2210412K19Rik; AA409584; d1401; mKIAA4192
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229303 representing NM_001252282
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTTCATTTAAGGACTTGTGCTGCTAAGTTAAGGCCATTGACAGCCTCCCAGACTGTTAAGACATTTT
  CACAAAACAAACCAGCAGCAATTAGGACGTTTCAACAGATTCGGTGCTATTCTGCACCTGTAGCTGCTGA
  ACCATTTCTTAGTGGGACTAGTTCGAACTATGTGGAGGAAATGTACTGTGCCTGGTTGGAGAATCCCAAA
  AGTGTACATAAGTCATGGGACATTTTTTTCCGAAACACCAATGCTGGAGCCCCACCGGGCACTGCCTACC
  AGAGCCCCCTTTCCCTGAGTCGAAGCTCCCTGGCTACCATGGCCCATGCACAGTCCCTGGTGGAAGCACA
  ACCTAACGTCGACAAACTCGTGGAGGACCACTTGGCGGTGCAGTCTCTCATCAGGGCATATCAGATACGA
  GGGCACCATGTAGCACAGCTGGACCCCCTGGGGATTTTGGATGCTGATCTGGACTCCTCCGTGCCCGCTG
  ACATTATCTCATCCACAGACAAACTTGATCTTGCAGTTTTCAAGGAACGACTTCGAATGCTAACAGTAGG
  AGGGTTCTATGGCCTACACGAGTCTGACCTTGACAAGGTCTTCCACTTACCCACCACCACTTTCATCGGG
  GGACAGGAGCCAGCACTTCCTCTTCGGGAGATCATCCGTCGGCTGGAGATGGCCTACTGCCAGCACATTG
  GTGTGGAGTTCATGTTCATTAATGATTTGGAACAATGCCAGTGGATCCGACAGAAGTTTGAGACCCCTGG
  AATCATGCAGTTCACCAATGAGGAGAAGCGGACCTTGCTGGCCAGGCTTGTACGATCCACCAGGTTTGAG
  GAGTTCCTACAGCGAAAGTGGTCCTCGGAGAAGCGTTTTGGTCTGGAAGGCTGTGAGGTGCTGATCCCTG
  CCCTCAAGACAATCATTGATATGTCAAGTGCAAATGGAGTGGACTATGTGATCATGGGGATGCCACACAG
  AGGACGGCTGAACGTGCTTGCAAATGTCATCAGGAAGGAGCTGGAGCAAATATTCTGTCAGTTTGACTCA
  AAGCTGGAGGCAGCTGATGAGGGTTCTGGGGACATGAAGTACCACCTGGGCATGTATCACCGCAGGATCA
  ACCGTGTGACCGACAGAAACATCACTTTGTCCTTGGTGGCTAACCCTTCCCATCTAGAGGCTGCTGACCC
  TGTCGTGATGGGAAAGACCAAAGCTGAACAGTTCTACTGTGGAGACACTGAAGGGAAAAAGGTGATGTCT
  ATCCTGCTGCATGGGGATGCTGCTTTTGCTGGCCAGGGCATCGTATATGAGACCTTCCATCTCAGCGACT
  TGCCGTCCTACACAACCCATGGCACTGTTCATGTGGTTGTCAACAACCAGATTGGCTTCACCACAGACCC
  TCGGATGGCCCGCTCCTCTCCCTACCCCACTGATGTGGCCCGAGTGGTGAATGCCCCCATTTTCCATGTC
  AACTCAGATGACCCTGAAGCTGTCATGTATGTATGCAAGGTGGCAGCTGAGTGGAGAAACACCTTCCACA
  AGGATGTTGTAGTTGATCTGGTGTGTTATCGACGAAATGGCCACAATGAGATGGACGAACCTATGTTTAC
  ACAGCCACTCATGTACAAGCAGATCCGCAAGCAGAAGCCTGTACTGCAGAAGTATGCAGAATTGCTAGTC
  TCCCAGGGTGTCGTCAATCAGCCTGAGTACGAGGAGGAAATCTCCAAGTATGATAAGATCTGTGAGGAAG
  CATTTACCAGATCCAAAGATGAGAAGATCTTGCACATCAAGCACTGGCTGGATTCCCCCTGGCCTGGCTT
  TTTCACCCTGGATGGACAGCCCAGGAGCATGACCTGCCCCTCCACTGGCCTGGAGGAGGATGTCTTGTTC
  CACATTGGAAAGGTGGCCAGCTCTGTACCTGTGGAGAACTTTACTATCCATGGAGGGCTGAGCCGGATCT
  TGAAGACCCGCAGAGAGCTTGTGACGAACCGGACTGTGGACTGGGCCCTGGCAGAGTACATGGCATTTGG
  CTCACTGCTGAAGGAAGGCATCCATGTGCGGCTGAGTGGCCAGGATGTGGAGCGGGGCACCTTCAGCCAT
  CGCCACCATGTGCTCCATGATCAGAATGTTGACAAAAGAACCTGCATCCCCATGAACCACCTTTGGCCAA
  ATCAGGCCCCTTACACTGTATGCAACAGCTCGCTGTCTGAGTACGGTGTCCTGGGCTTTGAGCTGGGCTT
  TGCCATGGCTAGCCCTAATGCTCTGGTTCTCTGGGAGGCCCAGTTTGGTGACTTCAACAACATGGCACAG
  TGCATCATTGACCAGTTCATCTGCCCAGGACAGGCAAAGTGGGTGCGGCAGAATGGCATTGTGCTCCTGC
  TGCCTCATGGCATGGAAGGCATGGGTCCCGAGCATTCCTCTGCCCGCCCAGAGCGGTTTCTGCAGATGTG
  CAATGATGACCCAGATGTCCTGCCTGACCTGCAGGAAGAAAACTTTGACATCAATCAGCTCTATGACTGC
  AACTGGATTGTTGTCAACTGTTCCACCCCTGGCAACTTCTTCCATGTGCTGCGACGACAGATCTTGCTGC
  CCTTCCGGAAGCCGTTAATCGTCTTCACTCCCAAATCCCTCCTGCGCCACCCTGAGGCAAGAACTAGCTT
  TGACGAGATGCTGCCAGGAACGCACTTCCAGCGTGTGATCCCAGAAAATGGACCTGCAGCTCAGGACCCA
  CACAAAGTCAAGAGACTTCTCTTCTGCACTGGGAAGGTGTACTATGACCTCACCCGAGAGCGCAAAGCCA
  GGAACATGGAGGAGGAGGTGGCTATTACAAGGATTGAGCAGCTATCACCATTCCCCTTTGACCTCCTGCT
  GAAAGAGGCACAGAAGTATCCCAATGCTGAGCTGGCCTGGTGCCAGGAAGAGCACAAGAACCAAGGCTAC
  TATGACTATGTCAAGCCAAGACTTCGTACCACCATTGACCGTGCTAAGCCTGTCTGGTATGCTGGCCGAG
  ACCCGGCAGCTGCTCCAGCCACTGGCAACAAGAAAACACACCTGACAGAGCTGCAGCGCTTTCTGGACAC
  AGCCTTTGACCTGGACGCATTCAAGAAATTCTCTTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001252282
• Insert Size: 3117 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001252282.1, NP_001239211.1
• RefSeq Size: 6675 bp
• RefSeq ORF: 3117 bp
• Locus ID: 18293
• UniProt ID: Q60597
• Cytogenetics: 11 A1
• Gene Summary: 2-oxoglutarate dehydrogenase (E1) component of the 2-oxoglutarate dehydrogenase complex, which mediates the decarboxylation of alpha-ketoglutarate. The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). The 2-oxoglutarate dehydrogenase complex is mainly active in the mitochondrion. A fraction of the 2-oxoglutarate dehydrogenase complex also localizes in the nucleus and is required for lysine succinylation of histones: associates with KAT2A on chromatin and provides succinyl-CoA to histone succinyltransferase KAT2A.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.