Egflam (NM_001289496) Mouse Untagged Clone

CAT#: MC229277

Egflam (untagged) - Mouse EGF-like, fibronectin type III and laminin G domains (Egflam), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Egflam
• Synonyms: 5930412K08; AU040377
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229277 representing NM_001289496
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATTTAATCTCAACCTTCTCGCTCCATTTCTTGCTCCTGGCCTGCAGCCTCCCACCCGGCGCTGTGT
  CACTCCGAACGGCTCTGCGAAAATCAGGCAAGGTGGGACCTCCTCTTGACATCAAGCTGGGAGCCTTGAA
  CTGTACAGCTTTCAGCATCCAGTGGAAAACACCAAAGCGTTCTGGTAGCTCCATCATTGGCTACACAGTG
  TTTTACTCTGAGGTTGGTTCAGATAAGTCCCTGCGGGAGCGGTCACACAACGTCCCGGTTGGCCAGGATA
  CCCTAATCACTGAGGAAGTGATTGGAGATTTGAAGCCAGGCACTGAATATCAAGTAAGCGTAGCAGCCTA
  CAGTCAGACTGGCAAGGGGAGGCTTAGCTTCCCTCGACATGTGACCACTTTGTCTCAAGATTCCTGCCTG
  CCCCCTGCAGCACCCCAACAGCCACATGTCCTTGTGGTTTCCGATTCTGAAGTGGCCCTTTCTTGGAGAC
  CTGGAGAGAATGAAGGAAGTGCCCCAATTCAGTCCTACTCTGTGGAATTCATTAGACCAGATTTTGACAA
  GAGCTGGACCATAATCCAAGAGCGCCTGCAGATGGACTCCATGGTTATCAAAGGCCTTGATCCAGATACC
  AACTACCAGTTTGCTGTGAAAGCCATGAATGCTCACGGCTTCAGTCCGCGCAGCTGGCCCAGCAACACCG
  TCCGAACCCTGGGTCCCGGGGAGGCTGGAAGTGGTCATTATGGACCTGGGTATATCACCAATCCCGGTGT
  GAGTGAAGATGATGATGGATCTGAAGACGAACTGGACCTGGATGTATCCTTTGAGGAGGTTAAACCACTT
  CCTGCTACCAAAGTTGGGAATAAGAAGTTCTCAGTGGAGTCCAAGAAGACTTCTGTCTCCAACTCAGTGA
  TGGGCTCCAGACTCGCCCAACCTACCTCAGCCTCTCTCCACGAAACTACAGTGGCCATCCCACCCACACC
  AGCCCAACGGAAGGGGAAGAACAGTGTGGCCATGATGTCAAGGCTCTTTGACATGTCCTGTGATGAAACT
  CTCTGTTCAGCTGACAGCTTCTGTGTCAATGACTACGCCTGGGGGGGCTCACGATGCCATTGCAACCTGG
  GCAAAGGAGGCGAGGCCTGCTCAGAAGATATCTTTATTCAGTATCCTCAGTTCTTTGGCCACTCCTACGT
  AACCTTTGAGCCTCTGAAGAACTCTTATCAGGCATTTCAGGTTACTCTGGAGTTCAGGGCAGAGGCGGAG
  GATGGCTTGCTGCTCTACTGCGGAGAGAGTGAGCACGGAAGAGGAGACTTCATGTCCCTGGCTCTCATCC
  GGCGGTCCCTCCACTTCAGGTTTAATTGTGGAACTGGGATTGCCATCATCATAAGTGAGACCAAAATCAA
  ACTTGGGGCCTGGCATACGGTGACACTGTACAGAGATGGCCTGAATGGGATGCTGCAGTTGAATAATGGC
  ACCCCAGTGACAGGCCAGTCCCAGGGTCAGTACAGTAAAATTACCTTCCGGACACCTCTGTACCTCGGGG
  GCGCTCCCAGTGCTTACTGGCTGGTCAGAGCAACAGGGACAAACCGTGGCTTCCAAGGCTGTGTGCAGTC
  ACTTTCTGTTAATGGGAAGAAGATTGACATGAGGCCCTGGCCATTAGGAAAAGCCCTCAATGGAGCTGAT
  GTTGGGGAGTGCAGCAGTGGGATCTGTGACGAAGCCTCCTGCATCCACGGGGGCACCTGCGCAGCCATCA
  AGGCCGACTCCTACATCTGCCTCTGTCCCCTTGGGTTCAGAGGTCGACACTGTGAAGATGCTTTTGCCCT
  GACCATCCCTCAGTTCAGAGAGTCGCTGAGGTCCTATGCTGCCACACCCTGGCCCCTGGAGCCTCAGCAT
  TATCTTTCTTTCACCGAGTTCGAGATCACCTTTCGGCCAGACTCGGGGGATGGTGTCCTTTTGTACAGCT
  ACGACACGGGCAGCAAGGACTTCCTCTCCATCAATATGGCTGCGGGCCACGTGGAATTCCGCTTCGACTG
  TGGTTCTGGGACGGGAGTTCTCAGGAGTGAGGCTCCCCTCACCCTAGGCCAATGGCATGACCTACGTGTG
  TCTCGCACAGCGAAGAATGGGATCTTACAGGTGGACAAGCAGAAGGTGGTAGAGGGAATGGCAGAGGGAG
  GCTTCACCCAGATTAAGTGTAATACGGACATTTTTATTGGTGGAGTCCCAAATTACGATGATGTCAAGAA
  GAACTCGGGGATCCTCCATCCATTTAGTGGGAGCATTCAGAAGATCATCCTCAACGACCGGACCATCCAT
  GTGAAGCATGACTTTACATCGGGCGTGAATGTGGAGAACGCAGCCCACCCCTGTGTAGGGGCCCCCTGTG
  CCCATGGGGGCAGCTGTCGGCCCAGGAAGGAGGGTTATGAGTGCGACTGTCCCTTGGGCTTCGAAGGCCT
  GAACTGCCAGAAAGAGTGTGGGAACCACTGCCTCAATACTATTATAGAAGCCATCGAGATCCCACAGTTC
  ATCGGCCGCAGCTACCTGACGTATGACAACCCAAATATCCTCAAGAGGGTGTCTGGATCGAGGTCAAATG
  CGTTCATGAGGTTTAAGACAACCGCCAAGGATGGCCTGTTGCTGTGGAGGGGGGACAGCCCCATGAGACC
  TAACAGTGACTTCATTTCCCTGGGTCTTCGGGATGGAGCCCTGATATTCAGCTACAACCTGGGCAGTGGC
  GTGGCTTCCATCATGGTGAACGGTTCCTTCAGCGATGGCCGGTGGCACCGAGTCAAGGCTGTCAGGGATG
  GCCAGTCCGGGAAGATTACAGTGGATGACTACGGAGCCAGAACAGGCAAGTCACCGGGCTTGATGCGGCA
  GCTTAACATCAACGGTGCTCTGTACGTGGGTGGAATGAAGGAAATCGCTCTGCACACGAACAGACAATAC
  CTGCGAGGCCTTGTGGGCTGCATCTCCCATTTCACACTGTCCACCGATTACCACATTTCCCTCGTGGAAG
  ACGCCGTGGATGGGAAGAACATCAACACTTGTGGGGCTAAGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001289496
• Insert Size: 3054 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001289496.1, NP_001276425.1
• RefSeq Size: 4888 bp
• RefSeq ORF: 3054 bp
• Locus ID: 268780
• UniProt ID: Q4VBE4
• Cytogenetics: 15 A1
• Gene Summary: Involved in both the retinal photoreceptor ribbon synapse formation and physiological functions of visual perception. Necessary for proper bipolar dendritic tip apposition to the photoreceptor ribbon synapse. Promotes matrix assembly and cell adhesiveness.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.