Rapgef4 (NM_001204165) Mouse Untagged Clone

CAT#: MC229271

Rapgef4 (untagged) - Mouse Rap guanine nucleotide exchange factor (GEF) 4 (Rapgef4), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rapgef4
• Synonyms: 1300003D15Rik; 5730402K07Rik; 6330581N18Rik; Epac-2; EPAC 2; Epac2; mKIAA4040
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229271 representing NM_001204165
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTCGCTGCGCACGCTGCACACTCTCAGTCCTCGGCCGAGTGGATCGCCTGCCTGGATAAAAGGCCGT
  TGGAGCGATCTAGTGAAGATGTGGACATAATTTTCACGCGGCTGAAAGGAGTTAAAGCTTTTGAGAAATT
  TCACCCAAACCTCCTTCGTCAGATTTGTTTATGCGGTTACTATGAGAACCTGGAAAAAGGAATCACACTG
  TTTCGCCAAGGGGATATTGGAACCAACTGGTATGCTGTCCTGGCTGGGTCTTTGGATGTTAAAGTGTCTG
  AGACCAGCAGTCACCAGGATGCGGTGACCATCTGCACTCTGGGAATTGGGACAGCCTTTGGAGAGTCCAT
  TCTGGATAACACCCCTCGCCATGCAACCATCGTTACCAGGGAGAGCAGCGAACTTCTCCGCATTGAGCAG
  GAGGACTTCAAGGCACTATGGGAGAAATACCGACAGTATATGGCCGGACTTCTGGCTCCTCCCTATGGTG
  TTATGGAAACGGGCTCTAACAATGACAGGATTCCTGACAAGGAGAATACACCTCTCATTGAACCCCACGT
  TCCTCTCCGTCCTGCTCACACCATTACCAAGGTCCCTTCAGAGAAGATCCTCAGAGCTGGAAAAATTTTA
  CGAATTGCCATTCTCTCTCGAGCTCCCCACATGATAAGAGACAGAAAGTACCACCTAAAGACATACAGAC
  AATGCTGTGTTGGGACTGAGCTGGTAGACTGGATGATACAGCAGACATCCTGTGTTCACTCGCGGACTCA
  AGCTGTTGGCATGTGGCAAGTCTTGCTGGAAGATGGTGTCCTCAACCATGTGGACCAGGAGCGCCATTTC
  CAAGACAAATATTTATTTTATCGATTTCTGGATGACGAGCGTGAGGATGCCCCTTTGCCTACTGAGGAAG
  AGAAGAAGGAGTGTGATGAAGAACTTCAGGACACCATGCTGCTGCTCTCACAGATGGGCCCTGACGCCCA
  CATGAGAATGATCCTGCGAAAACCACCTGGCCAGAGGACTGTGGATGACCTAGAGATTATCTACGACGAG
  CTCCTTCATATTAAAGCCTTATCCCATCTCTCTACCACAGTGAAACGGGAGTTAGCAGGTGTTCTCATTT
  TTGAGTCTCACGCCAAAGGAGGAACTGTGTTGTTTAACCAGGGGGAAGAAGGTACCTCCTGGTACATCAT
  TCTGAAAGGATCCGTGAATGTAGTCATTTATGGCAAGGGTGTGGTCTGCACCCTGCACGAAGGAGATGAC
  TTTGGCAAGTTAGCTCTAGTGAACGATGCTCCAAGAGCTGCCTCCATTGTTCTTCGGGAAGATAATTGTC
  ACTTCCTAAGAGTCGACAAGGAAGACTTCAATCGGATTCTGAGGGACGTTGAGGCGAATACAGTCAGACT
  TAAAGAACATGACCAAGATGTCTTGGTACTGGAGAAGGTCCCAGCAGGGAACAGAGCTGCTAATCAAGGA
  AACTCACAGCCTCAGCAAAAGTATACTGTGATGTCAGGAACACCTGAAAAGATTTTAGAGCATTTTCTAG
  AAACAATACGCCTTGAGCCATCGTTGAATGAAGCAACAGATTCGGTTTTAAATGACTTTGTTATGATGCA
  CTGTGTTTTTATGCCAAATACCCAGCTTTGCCCTGCCCTTGTGGCCCATTACCACGCACAGCCTTCTCAA
  GGTACCGAGCAGGAGAGAATGGATTATGCCCTCAACAACAAGAGGCGGGTCATCCGCTTGGTCCTGCAGT
  GGGCGGCCATGTATGGCGATCTCCTCCAAGAAGATGATGTGGCCATGGCCTTCCTGGAGGAGTTCTATGT
  GTCTGTATCAGATGACGCACGGATGATGGCTGCCTTCAAGGAGCAGCTGCCAGAGCTGGAGAAGATTGTC
  AAGCAAATCTCAGAAGACGCAAAAGCTCCACAGAAGAAGCACAAGGTGCTTTTGCAACAGTTCAACACAG
  GTGACGAGAGGGCCCAGAAGCGTCAGCCTATTCGTGGCTCTGATGAGGTTTTGTTCAAGGTCTACTGCAT
  CGACCACACCTATACTACCATTCGTGTGCCGGTAGCTGCCTCGGTGAAGGAAGTCATCAGTGCAGTAGCT
  GACAAACTGGGCTCAGGGGAAGGCCTGATCATCGTCAAGATGAACTCTGGAGGAGAAAAGGTGGTGCTGA
  AATCTAATGATGTTTCAGTATTTACGACGCTCACCATTAATGGACGCCTGTTTGCCTGCCCGAGAGAGCA
  ATTCGACTCACTGACTCCCTTGCCGGAACAGGAAGGCCCGACCACTGGGACAGTGGGAACATTTGAGCTG
  ATGAGCTCGAAAGACCTGGCGTACCAGATGACAACCTACGATTGGGAACTCTTCAACTGTGTGCATGAGC
  TGGAGCTAATCTACCACACATTTGGAAGGCATAATTTTAAAAAGACCACGGCAAACTTGGATTTGTTCCT
  GAGGAGGTTTAATGAAATTCAGTTTTGGGTTGTCACTGAGGTCTGCCTTTGTTCCCAGCTCAGCAAACGT
  GTTCAGCTTTTGAAAAAATTTATCAAGATAGCGGCTCACTGCAAGGAGTACAAAAATCTAAATTCCTTTT
  TCGCCATCGTCATGGGACTCAGCAACGTGGCCGTGAGCCGCTTGGCACTAACGTGGGAGAAACTGCCGAG
  CAAGTTTAAGAAGTTCTATGCGGAGTTTGAGAGCTTGATGGATCCTTCCAGAAACCACAGGGCATACAGG
  CTGACAGCAGCCAAGCTGGAGCCCCCTCTCATCCCTTTCATGCCCTTGCTTATTAAAGATATGACATTTA
  CTCATGAGGGGAACAAGACGTTCATTGACAATCTAGTAAACTTTGAAAAAATGCGCATGATTGCAAACAC
  TGCCAGAACAGTACGGTACTACAGGAGCCAGCCCTTCAATCCGGATGCCGCTCAAGCTAATAAGAACCAT
  CAGGATGTCCGGAGTTATGTACGGCAATTAAATGTGATTGACAACCAGAGAACTTTATCACAGATGTCAC
  ACAGATTAGAGCCTCGAAGGCCATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001204165
• Insert Size: 3036 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001204165.1, NP_001191094.1
• RefSeq Size: 4220 bp
• RefSeq ORF: 3036 bp
• Locus ID: 56508
• UniProt ID: Q9EQZ6
• Cytogenetics: 2 C3
• Gene Summary: Guanine nucleotide exchange factor (GEF) for RAP1A, RAP1B and RAP2A small GTPases that is activated by binding cAMP. Seems not to activate RAB3A. Involved in cAMP-dependent, PKA-independent exocytosis through interaction with RIMS2.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).