Palld (NM_001293773) Mouse Untagged Clone

CAT#: MC229262

Palld (untagged) - Mouse palladin, cytoskeletal associated protein (Palld), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Palld
• Synonyms: 2410003B16Rik; 6030492A02
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229262 representing NM_001293773
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCACAAGCTCAGAAGAAAACAACGTCCGTTTCCTTAACGATAGGATCCTCAGCTCCGAAAACAGGAG
  TGACCACAGCTGTGATTCAGCCCCTATCTGTGCCCGTCCAACAGGCTCACAGCGCTACTTCATATCTCTG
  CCGACCTGACGGAACCACCATGGGCTGCCTTCTTCCTGTTTTTACTAAGGAACTACAGAACACAGCAGCC
  TCCGAGGGCCAGGTGGTGGTGCTGGAATGCAGAGTCCGAGGGGCACCCCCGCTGCAGGTCCAGTGGTTCC
  GGCAAGGAAGTGAGATCCAAGACTCTCCGGACTTCAGAATTCTCCAGAAAAAACCCAGATCGACAGCTGA
  ACCCGAGGAGATCTGCACCCTTGTCATTGCTGAGAGTTTCCCTGAAGATGCAGGCATCTTTACCTGCTCA
  GCCACAAATGACTACGGCTCAGTGACCAGCACTGCACAGCTTGTTATAACCTCCGCCAACAACGAGAACT
  GTAGTTACGACTCAACAGGAGAACCCAACAGTGATCACTTCCAACACTTTCCACCTCCCCCTCCGATCCT
  GGAGACAGGCTCCTATGAGCTGGCATCCCAGAAACCATCTGAAATCCAGCAGGTGAACAGCCCCAATTTA
  GGATTTAGCATGGCAGCTCTTCAAATGCAATTCAACACTGCAGAGAGGGAGACCAACGGAGTCCATCCCA
  GCCATGGAGTCAACGGGCTGATTAATGGCAAAGCTTATGGCAATAAATCTCCTCCAACACCAACTGCCCT
  GCTTTCACCCACTAAGGAACCACCGCCACTCCTTGCCAAACCCAAACTGGATCCTCTGAAGCTTCAGCAG
  CTGCAGAACCAAGTGCGCCTGGAACAGGAGGCCTGCGCCTGGCCCCCAGCGCCCCCAGGTGTCCCTTGCA
  ACAGCAGCAGCAGCGGCAGCAGCGCGCCCCCGTCGCCGCCCTTCCCCCCACCGCCACCAGCCTTTCCGGA
  GCTCGCGGCCTGTGCGTCGCCGGTGCCCTCGGAGCCCATGAGCGCTCTGGCCTCCCGCGCTACCGCCATG
  CAGTCCTCCGGCTCCTTCAACTACGCGCGCCCTAAGCAGTTCATCGCGGCGCAGAACCTGGGTCCCGCAT
  CGGGGCTGCCCACGCCAACCTCCAGCCCCAGCTCCTCCAGCTTGCCGTCACCATTGTCCCCCACGCCCAG
  GCCCTTCGGCCGCGCGCCCGGGCCGCCCTTCGTGGAACCCGAGGCCATGTGGGGCCCGTCCTCGCCCTCG
  CCGCCACCGCCGCCACCTCCGGTCTTCAGCCCCTCTGCAGCCTACCCGGTGCCCGACGTGTTCCCACTGC
  CACCGCCGCCGCCACCGCTACCTAGCTCCACCTCACACTGCGCCTCGCCCGCCCGCTTCGGCCCCAGCCA
  GACGCCCGCAGCCTTCCTCAGCGCCCTGCTGCCCTCTCAGCCACCGCCTGTCGCTGTCAACGCCCTGGGG
  CTGCCCAAGGGAGTCACCCCCGCGGGGTTCCCAAAGAAGTCCAGTAGAACTGCTAGAATTGCCTCTGATG
  AGGAGATTCAAGGCACAAAGGATGCTGTCATCCAAGACCTGGAACGGAAGCTTCGCTTCAAGGAGGACCT
  TCTGAACAATGGCCAACCGAGGCTAACCTATGAGGAAAGAATGGCTCGCCGCCTGCTTGGAGCCGACAGC
  GCAAACGTCTTCAACATCCAGGAGCCAGAGGAAACGGCAGCCAATCAGGAGTACAAAGTCTCTAGCTGCG
  AGCAGAGGCTGATTAGCGAGATTGAGTACCGGCTGGAGCGCTCCCCTGTGGATGAGTCGGGAGACGAGGT
  GCAGGATCCAGATGTGCCTGTGGAGAACGCAACAGCTCCCTTCTTTGAGATGAAGCTGAAACACTACAAG
  ATCTTTGAGGGGATGCCGGTGACTTTCACGTGTCGAGTGGCTGGGAATCCAAAGCCAAAGATCTATTGGT
  TTAAAGATGGGAAGCAGATTTCTCCGAAGAGCGATCACTACACCATTCAGAGAGACCTTGATGGGACCTG
  CTCTCTCCACACCACGGCCTCTACCCTAGACGACGATGGGAACTACACCATCATGGCTGCCAACCCTCAG
  GGTCGCGTCAGTTGTACAGGAAGGCTAATGGTACAGGCTGTCAACCAAAGAGGCCGCAGTCCCCGCTCTC
  CCTCAGGCCATCCTCATGCCAGAAGGCCTCGCTCTCGATCACGGGACAGTGGAGATGAAAACGAGCCCAT
  TCAGGAGCGATTCTTCAGACCTCACTTCCTGCAGGCTCCTGGAGACCTGACCGTTCAGGAAGGCAAGCTC
  TGCAGGATGGACTGCAAGGTCAGTGGGTTACCAACCCCAGATCTCAGCTGGCAACTAGATGGAAAGCCCA
  TACGCCCCGACAGTGCTCACAAGATGCTGGTCCGTGAGAATGGGGTCCACTCCCTCATTATAGAGCCAGT
  CACGTCCCGGGACGCCGGCATCTACACATGTATTGCCACCAACAGAGCAGGACAGAACTCGTTTAACCTG
  GAGCTTGTGGTTGCTGCTAAGGAAGCACACAAGGCCCCTGTGTTTATGGAGAAGCTACAGAACACGGGGG
  TTGCTGATGGATACCCAGTGCGGCTGGAATGCCGTGTCTCGGGAGTGCCGCCACCTCAGATATTTTGGAA
  GAAAGAAAATGAATCGCTCACTCACAGCACTGAGCGAGTAAGCATGCACCAGGATAATCATGGCTACATC
  TGCCTGCTCATCCAGGGAGCCACAAAGGAAGACGCTGGGTGGTACACTGTGTCCGCCAAGAACGAAGCAG
  GCATTGTGTCCTGCACTGCCCGGCTGGATGTCTACACCCAGTGGCACCAGCAGCCACAGACCACCAAGCC
  AAAAAAAGTACGGCCCTCGGCCAGTCGCTACGCAGCACTTTCGGACCAGGGACTAGACATCAAAGCCGCT
  TTCCAACCTGAAGCCAGCCCATCTCACCTGACTCTGAACAGCGGCTTGGTAGAAAGTGAAGACCTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001293773
• Insert Size: 3009 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001293773.1, NP_001280702.1
• RefSeq Size: 5458 bp
• RefSeq ORF: 3009 bp
• Locus ID: 72333
• Cytogenetics: 8 B3.1
• Gene Summary: Cytoskeletal protein required for organization of normal actin cytoskeleton. Roles in establishing cell morphology, motility, cell adhesion and cell-extracellular matrix interactions in a variety of cell types. May function as a scaffolding molecule with the potential to influence both actin polymerization and the assembly of existing actin filaments into higher-order arrays. Binds to proteins that bind to either monomeric or filamentous actin. Localizes at sites where active actin remodeling takes place, such as lamellipodia and membrane ruffles. Different isoforms may have functional differences. Involved in the control of morphological and cytoskeletal changes associated with dendritic cell maturation. Involved in targeting ACTN to specific May be required for the initiation of neural tube closure.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence and lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) is shorter at the N-terminus and lacks an alternate internal segment compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.