Trim24 (NM_001272076) Mouse Untagged Clone

CAT#: MC229247

Trim24 (untagged) - Mouse tripartite motif-containing 24 (Trim24), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Trim24
• Synonyms: A130082H20Rik; AI447469; D430004I05Rik; Tif; TIF1; TIF1-alpha; Tif1a; TIF1alpha
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229247 representing NM_001272076
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGATATTTTTGATTACACTTTTATCGGTGGGTGTGGAGATGCTTGTCCCACATGCGCATGTGGAA
  GTCAGAGGATAACTTGGAAAAGTCAGTTCTTTCCATCCTGTGTTCGTCCCATGGATAAAACTGCTGTCAC
  TGGTGATGAGCAAGTTGGAGTCATTCGATGCCCAGTTTGCAGTCAAGAGTGTGCTGAGAGACACATCATA
  GACAACTTTTTTGTGAAGGACACCACTGAAGTTCCTAGTAGTACAGTAGAAAAGTCTAATCAGGTATGTA
  CAAGCTGTGAAGACAATGCAGAAGCTAATGGGTTTTGTGTAGAGTGTGTTGAATGGCTCTGCAAGACATG
  TATTAGAGCTCACCAGAGGGTGAAGTTCACAAAAGACCACACAGTCAGGCAGAAAGAAGAAGTATCTCCA
  GAGGCAGTTGGGGTGACCAGTCAGCGACCAGTGTTTTGTCCCTTCCATAAAAAGGAGCAGTTGAAACTTT
  ACTGTGAAACATGTGATAAACTGACCTGTCGAGACTGCCAGCTGCTAGAACACAAAGAACACAGGTATCA
  ATTTATAGAAGAAGCTTTTCAGAATCAAAAAGTGATCATAGATACTCTAATCACCAAACTGATGGAAAAA
  ACAAAATATATAAAGTATACAGGAAATCAGATCCAAAATAGGATAATTGAAATAAATCAAAACCAAAAGC
  AGGTGGAACAGGATATTAAAGTTGCCATCTTCACATTGATGGTGGAGATAAACAAAAAAGGGAAAGCTCT
  GCTGCACCAGCTTGAGAGTCTTGCAAAGGACCATCGAATGAAACTCATGCAACAACAGCAGGAAGTGGCT
  GGGCTTTCTAAGCAGTTAGAGCACGTCATGCATTTTTCTAAATGGGCTGTTTCCAGTGGCAGCAGCACAG
  CCTTGCTGTACAGCAAGCGGCTGATTACATACAGGTTACGGCACCTTCTTCGTGCAAGGTGTGATGCTTC
  TCCTGTGACCAACACCACCATCCAGTTTCACTGTGATCCTAGTTTCTGGGCTCAAAATATTATCAACTTG
  GGTTCTTTAGTAATCGAGGATAAAGAGAGCCAGCCACAAATGCCTAAGCAGAATCCTGTCGTGGAGCAGA
  GTTCACAGCCACCAGGTGGTTTACCTTCCAACCAGTTATCCAAGTTCCCAACACAGATCAGCCTAGCTCA
  GTTACGACTCCAGCATATTCAGCAACAGGTAATGGCTCAGAGGCAACAGGTGCAACGGAGGCCAGCACCT
  GTGGGTTTACCAAACCCTAGAATGCAGGGGCCCATCCAGCAGCCTTCCATCTCTCATCAGCATCCGCCAC
  CACGCTTAATAAACTTTCAGAATCACAGCCCTAAGCCCAATGGACCAGTTCTTCCTCCTTATCCTCAGCA
  GCTGAGATATTCACCAAGCCAGAATGTACCTCGGCAGACAACAATAAAGCCCAACCCCTTGCAAATGGCT
  TTTTTGGCTCAACAGGCCATAAAACAGTGGCAGATCAGCAGTGTACAGGCTCCGCCCACAACTGCCAGCA
  GCTCCTCCTCCACGCCGTCCAGCCCCACAATCACAAGTGCAGCTGGGTACGATGGAAAAGCTTTTAGTTC
  ACCCATGATTGATCTGAGTGCACCGGTGGGAGGGTCTTACAATCTTCCTTCTCTTCCAGATATTGATTGT
  TCAAGTACTATAATGTTGGACAACATTGCAAGGAAAGACACAGGTGTAGATCACGCCCAGCCGAGGCCTC
  CGTCAAACAGAACGGTGCAGTCACCAAATTCATCAGTGCCATCTCCAGGCCTTGCAGGGCCTGTTACTAT
  GACTAGCGTCCATCCCCCAATACGTTCACCTAGTGCCTCCAGTGTTGGAAGTCGAGGAAGCTCTGGCTCT
  TCCAGCAAACCAGCAGGAGCTGATTCTACTCACAAGGTCCCAGTAGTCATGCTGGAGCCAATTCGAATAA
  AACAGGAAAACAGTGGACCACCTGAAAATTATGATTTTCCTGTTGTTATAGTAAAACAAGAATCAGATGA
  AGAATCTAGACCTCAAAATACTAACTATCCAAGAAGCATACTTACCTCCCTCCTCTTAAACAGCAGTCAG
  AGCTCTGCTTCTGAGGAAACCGTGTTACGATCTGATGCCCCTGATAGTACAGGAGATCAGCCTGGACTCC
  ATCAAGAAAATTCCTCAAATGGAAAGTCTGAGTGGTCGGATGCCTCCCAGAAGTCCCCTGTGCATGTCGG
  AGAGACGAGGAAGGAGGATGACCCCAATGAAGACTGGTGTGCTGTTTGTCAAAATGGTGGGGAACTCCTA
  TGCTGTGAGAAATGTCCTAAAGTATTCCATCTTACTTGTCATGTGCCCACCTTGACAAATTTTCCAAGTG
  GAGAATGGATCTGTACTTTCTGCCGAGACTTATCTAAGCCAGAGGTTGACTATGATTGTGATGTTCCCAG
  TCACCACTCAGAGAAACGGAAAAGTGAAGGCCTTACTAAGTTAACGCCAATAGACAAAAGGAAATGTGAA
  CGCCTACTTCTGTTTCTTTACTGCCATGAAATGAGCCTGGCTTTCCAAGACCCTGTTCCTCTAACTGTGC
  CTGATTATTATAAAATAATTAAAAACCCAATGGACTTGTCAACCATCAAGAAAAGACTTCAGGAGGATTA
  TTGCATGTATACAAAGCCTGAAGACTTTGTAGCTGATTTTAGATTGATCTTTCAAAACTGTGCTGAATTC
  AATGAGCCTGATTCTGAAGTAGCCAATGCTGGTATAAAACTTGAAAGCTATTTTGAAGAACTTCTAAAGA
  ATCTTTATCCAGAAAAAAGGTTTCCTAAGGTAGAATTCAGGCATGAAGCAGAAGACTGTAAGTTCAGTGA
  CGACTCAGACGATGACTTTGTACAGCCCCGGAAGAAGCGTCTCAAGAGCACCGAGGATCGCCAGCTGCTT
  AAGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001272076
• Insert Size: 2946 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001272076.1, NP_001259005.1
• RefSeq Size: 5771 bp
• RefSeq ORF: 2946 bp
• Locus ID: 21848
• UniProt ID: Q64127
• Cytogenetics: 6 B1
• Gene Summary: The protein encoded by this gene is part of the tripartite-motif containing family (TRIM), which are typified by the RING, B-box type 1, B-box type 2, and coiled-coil region domains. This protein, which also contains a PHD/TTC finger and bromodomain important for regulating nuclear receptors and binding chromatin, has important roles in differentiation, development, and tissue homeostasis. This protein has been reported to regulate the activity of the tumor suppressor p53 and of the retinoic acid receptor. A translocation event between this gene and Braf transforming gene, which results in the fusion protein T18, has been reported in hepatocellular carcinomas. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Jan 2013]
  Transcript Variant: This variant (3) differs in the 5' UTR and 5' coding region, and uses an alternate start codon, compared to variant 1. The encoded isoform (3) has a distinct and shorter N-terminus that lacks the N-terminal RING domain, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.