Inpp4b (NM_001297596) Mouse Untagged Clone

CAT#: MC229184

Inpp4b (untagged) - Mouse inositol polyphosphate-4-phosphatase, type II (Inpp4b), transcript variant 4

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Inpp4b
• Synonyms: E130107I17Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229184 representing NM_001297596
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAATCAAAGAGGAAGGAACATCAGAAGAAGGCCAGCATTTTCTTCCTGCAGCTCAGGCAAATGACC
  CTGAGGACATTCAGTTTACCAGCATCCAGAAGATTCCGAACGAGCCACAGTTGGAATTCATTCTCGCCTG
  CAGGGATCTTGTGGCTCCTGTCAGTGATCGGAAACTGAATACAGTAGTCCAGATCTCAGTAATCCACCCG
  GTGGAGCAGACCCTGACGAGGTACTCCAGCACAGAAATTGTGGAGGGAACAAAAGACCCACTGTTCCTAA
  CGGGTGTCACATTCCCACCGGACTACCCCATCTATGAAGAGACAAGAATAAAACTGACAGTCTACGATGT
  CAAGGATAAGTCTCATGATACTATTCGAACCAGTGTCCTTCCGGAACACAAGGATCCCCTGCCAGAAGTT
  GCGAGAAGCTTCCTGGGCTGTGCCAGTTTTAAAGTTGGGGAGCTGCTGAAGTCCAAGGAGCAGCTGCTGT
  CCCTGAGCCTGAGAACCTCAGATGGTGGCAAAGTGGTCGGCACCATAGAAGTCAGCCTCGTGAAGATGGG
  AGAGATTGAGGATGGGGACACCGACCACATCACAACAGATGTGCAGGGACAAAAGTGTGCACTAATGTAT
  GAATCCACAGCACCAGAAAGTCTGAGCGGGAAGGAAAACTTACCTTTTATGAATGCAGTCTTAAGGAATC
  CTGTGTGTAAGCTGTATAGATTCCCCACATCTGACAATAAGTGGATGCGAATCCGTGAGCAGATGTCAGA
  AAGCATCCTTTCTTTTCATATTCCTAAGGAATTGATTTCCCTTCACATAAAAGAAGACTTATGTAGAAAC
  CAGGAGTTAAAAGAACTTGGTGACCTTTCCCCACACTGGGACAACCTACGGAAAAATGTCCTTTCTCACT
  GTGATCAAATGGTGACTATGTACCAAGACATTCTGACAGAACTTTCCAAAGAAACAGGGTCCTCTTTCAA
  ATCCAGCAGCAGCAAAGGAGAGAAAACATTAGAATTTGTTCCAGTAAATCTACACTTGCAACGGATGCAG
  GTGCACAGCCCTCACCTGAAAGATGCTCTCTATGATGTCATCACTGTGGGGGCCCCAGCTGCCCATTTTC
  AGGGATTCAAGAACGGTGGTCTTCGTAAACTCCTCCATAGGTTTGAGACAGAGAGAAGGAACACCGGCTA
  CCAGTTTATTTACTATTCACCTGAGAACACAGCGAAGGCAAAGGAAGTTCTCAGCAGCATCAATCAGCTA
  CAACCTCTCATAGCAACTCATGCAGACCTGCTGCTAACCTCTGCCAGCCAGCGTTCTCCAGACAGCCTGA
  AGAGCTCTTTAAAGCTGCTTTCAGAAAAAACTGAGCTTTTTGTCCATGCCTTCAAGGACCAGCTGGTCAG
  GAGTGCTCTTTTAGCTCTCTACACTGCAAGGCCAGGAGGCATCCTTAAGAAGCCACCCTCTCCTAATGTC
  AGCACAGAGGAGAAAAGTACTCAGCATGATACCCCACAGCTTAGAAGACAAGACTCCATACCACATCATT
  CTGACTATGATGAAGAAGAGTGGGATAGGGTGTGGGCCAACGTGGGGAAGAGCCTGAACTGCATTATCGC
  TAAAGTAGACAAACTGATCGAGAGAGACAGTCACAACGAAGAAGGTGCTGGAGGCAGCAGTAGCAAGGAT
  GGAGAGGCAGACCATACCCTGGAAGATTCCATCACATCTCACCCACGTGAGGACTGGTATGAACAGCTGC
  ACCCACTCATCCTTACCTTGAAGGAGTGCATGGGAGAAGTGGTGAACCGAGCAAAGCAATCCCTGACATT
  TGTACTGCTTCAGGAACTGGCCTACAGTCTGCCCCAGTGTTTGATGCTGACTCTGAGAAGAGACATTGTC
  TTCAGTCAGGCGCTTGCTGGATTGGTTTGTGGTTTTATCATCAAATTACATACAAGTTTGCATGACCCTG
  GCTTCCTACAGCAGCTTCATACAGTGGGCTTGATAGTACAATACGAAGGACTGCTAAGTACTTACAGTGA
  TGAAATTGGAATGCTGGAGGACATGGCAGTCGGCATTTCAGATTTGAGGAAAGTTGCATTTAAAATAACT
  GAAGCCACGTCCAATGATGTCCTTCCTGTTCTCACGGGGAGACGGGAACACTATGTGGTGGAGGTCAAGC
  TTCCAGCTACAGTATTTGAGTCGCTCCCACTGCAGATTAAAGAAGGCCAGTTGCTTCATGTGTATCCCGT
  TCTGTTTAATGTCGGAATCAATGAGCAGCAGACTCTGGCTGAGAGGTTTGGAGATGTTTCTTTGCAAGAA
  AGTATTAATCAAGAAAATTTTGAACTTGTACAAGAATATTATTCAATATTTATGGAAAAGATGCCTCCTG
  ATTATATTTCACATTTTCAAGAACAAACTGATTTAAAAGGACTGTTGGACAATCTCCACCAAAATATTCA
  AGCCAAAAAAAGAAAGAATGTAGAAATCATGTGGCTGGCAGCAACGATTTGTCGGAAACTCAATGGCATT
  CGTTTTACCTGCTGTAAAAGTGCCAAAGACAGGACATCGATGTCGGTGACACTGGAACAGTGCTCCATCC
  TGAGAGACGAGCATCAACTGCACAAAGACTTTTTCATCAGAGCTTTAGATTGCATGAGAAGAGAAGGATG
  CCGCATAGAGAATGTACTGAAGAATATCAAATGCAGGAGGTATGCTTTCAACATGCTACAGCTGATGGCT
  TTCCCCAAGTGCTACAGACCTCCAGAAGGCACTTATGGAAAAGCTGACACCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001297596
• Insert Size: 2784 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001297596.1, NP_001284525.1
• RefSeq Size: 8367 bp
• RefSeq ORF: 2784 bp
• Locus ID: 234515
• Cytogenetics: 8 39.02 cM
• Gene Summary: Catalyzes the hydrolysis of the 4-position phosphate of phosphatidylinositol 3,4-bisphosphate, inositol 1,3,4-trisphosphate and inositol 3,4-bisphosphate (By similarity). Plays a role in the late stages of macropinocytosis by dephosphorylating phosphatidylinositol 3,4-bisphosphate in membrane ruffles (By similarity). Antagonizes the PI3K-AKT/PKB signaling pathway by dephosphorylating phosphoinositides and thereby modulating cell cycle progression and cell survival (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (4) uses an alternate last exon compared to variant 1. The resulting isoform (4) has a shorter and distinct C-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.