Zfp473 (NM_001289837) Mouse Untagged Clone

CAT#: MC229116

Zfp473 (untagged) - Mouse zinc finger protein 473 (Zfp473), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Zfp473
• Synonyms: D030014N22Rik; zfp-100; Znf473
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229116 representing NM_001289837
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAGGAAGGAGGACGACCTTAAAGGAGGTTGCTGGAATCAGCCAGCTGTGGCTGAGGAGTTTGAGA
  CCCTCAAGGATTTAGCCATGGATTTCACCGTGGAAGACTGGAAAGACCTGGAGTCAGAGTGGGACCAGAG
  AGACCTGTTCTGGGATGTGACGCTGAACCACTACCAGGACATGTTCTCATTTACAGATACTTCGCAACCC
  AGCCTGACTTCCCAGCCAGATGTCAGGGAAGAGCTGGAAGCCACCAGTACTGAAGTGCCTGAGACCAAGA
  GCTCTCCTTTGCAGAGTGGTTTCGTAGAAGAAGACTTCTCCCAGATTATGGAGATATTTTCCAACGGGCA
  ACTCAACTTTGAAGCCTGTATAGGTGAGGATTGGCTAAATAGTTTTCTCGGAGATCCAGAAAGTCTTCCA
  AGACCTGACATCAGCGACAAGGAAAGCCCCGCGGATCATCAGAGTCCTGAGTCCAAGAGCGGCCTCAGTC
  CTGGGCCTCCCCTTTGCACAAGAGAGGATGCTGTGATGTCTGCTAGTCCTGAAAAGACCCTCACTCCAGT
  GATACTGAAGGAATCCAGGAGTGACCTGAGCCAGGAGGACTCTGTCCAGGGACACGAGAAGCCATATAAG
  TGTAGTGAGTGTGGGGAAAGCTTCAGCCAGAGTCACCACCTCATCCAGCACTGGGTTCTCCACACCTCAG
  GGGAGCCTCCTATATGGCGAGAGCAGCAGCGAGGTCTCAGCCAGGGTGCTCACTTTCCCATGTGTCCAGG
  GACTCCAGCAAGCTATGAATCCTACACGTGTCAGGAGTGTGGGAAAAGGTTTAGTCAGAATGTGTACCTC
  CAGTGGCATCAGAAAATCCACACTGGAGAAAAACTTTGTAAAACTCAAAGTGACAGCAACCTAGAAGGGC
  TCTCTAGGAGTCCGAGTGTCGAGCCAGGAAAGCAGCGGCTGAGTAAAGACACTGACTCAGCAAAACCGAG
  TACCATTCACGGTCAGGACCAGGAAAAACCACCTACTGGTGAAAGTCGTGACCAAGAAAACCTGCATGAG
  AGCCAGCCTGGTGACAGGCCATCCGTTCTTCATCCAAAGCCTCTTAGGCATCAGAAGACTCCTACAAACG
  CTAAGTGCTTCAGATGTAAGAAGTGTGGGGAGACATTCAGTGGGGCCTTTCATCTAGCTAAACACCAGAG
  AGCCCATGCTCAGAGACTCTACAAGTGCGCTTCGTGCCCTGCCGTCTTCAACCTAAGCAAGCATTGCTTC
  CAGCATCGCAAGTCACATTTCCCCAGCGCGGCCTGCGAGTGTCAGGGATGCAGAAAGAGCTTTAATTGGC
  GGTCATCACTCATTAAGCACCAGGCTATTCACAAAGGAGAGAAGCCGTACAAGTGTGATGAATGTGGTAA
  AGCTTTCAACCACTCCTCCACCCTGAAGATCCACCAGAGGATCCACAGTGGACAGAAGCCTCACAAATGC
  AGTGAGTGCGGGAAAGCCTTCTGTCGGAGAACAGATCTTACTGAGCATCAGCGAGTCCACTCGGGCTTCA
  GACCCCATCAGTGTCCCGTGTGTGCCAGGACTTTTAACCGACCTTCACACTTAGTTCGACACCGGCTGAG
  ACATGCTGAAGAAAGGCACTTTGGCTGTGCTAAATGCAAGGAGACCTTCATCTATAAAGAACAGCTAGAG
  CGGCACAATAAAATCCACACCATCGAGGGCTTGTATGAGTGTAAGCAGTGTGGAGAACACTTCATTTGCA
  GGTCAACTCTGAATTGCCACCTGAGCATACACATTAGAGAAAATACAAGTGAGAAAGTTGTGGGTCAGAA
  CTCACAGCACACTGAAAAATGCTTTAAGAATACCAAGTGTAGAAAAGCCCCTAACCACAGCAGATACCTA
  GGGCAGCATGAGAAGATTCATGCCCAGGTGACATCCGGTGAGTGTGACCCATGTGGGGAAACCTATGACC
  AGAGTGTGCAGCCCATTTGCCATCAGAGCATCTGTGCTGGTGTGAAACCAAGTGAATGCGCTGAGCCTGA
  GAAATGCACGCGAAACACCTCTGCCAGTGAGCACCATCCTTCCCAAAGGGAACCATCCTTCAAGTGTGAC
  ATATACAACAGGGCCTTCAAACAGCGTGCCCACCTTTCAAAACATCAGTTGATTCACATCACTGAGAAGC
  CCTTTAAATGCAACGAATGTGACAGAGCCTTCAAGCAGAGTAACTACCTCATTCAGCATCAGAAGACTCA
  CACTGCAGAGAAGCACTTTGAATGTAGCGAGTGTGGGAAAACATTTCATCAGCGATCATGCCTTTCTAAG
  CACCAGAAAATCCACTCAGGGGAGAAGCCCTTTAAGTGTGGTGACTGTGGGAAAGCCTTCATCTCTGGTG
  CCCAGCTCATCCGACACCAGAGAATTCACACTGGGGAGAAGCCGTACGTCTGCCAGGAATGTGGGAAAAC
  CTTCAGCCAGAGCTCGTGCCTTACCCTTCACCTTAGAATTCACACTGGGGAGAAGCCGTACACATGCGGC
  ACGTGCGGGAAAGCCTTTGCTCAGAGAGCAAACCAGAGGAAGCACGAGAGAATTCACACTGGGGAGAAGC
  CCTATGCCTGCGGGCTGTGTGGGAAGGCCTTCGGCCTCCGCACCCATCTCCAGCAACACCAGAGAATTCA
  CACCAAGGCCAAACCATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001289837
• Insert Size: 2679 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001289837.1, NP_001276766.1
• RefSeq Size: 3957 bp
• RefSeq ORF: 2679 bp
• Locus ID: 243963
• UniProt ID: Q8BI67
• Cytogenetics: 7 B3
• Gene Summary: Involved in histone 3'-end pre-mRNA processing by associating with U7 snRNP and interacting with SLBP/pre-mRNA complex. Increases histone 3'-end pre-mRNA processing but has no effect on U7 snRNP levels, when overexpressed. Required for cell cycle progression from G1 to S phases (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1, 2 and 3 encode the same isoform (1).