Arhgap27 (NM_001205236) Mouse Untagged Clone

CAT#: MC229081

Arhgap27 (untagged) - Mouse Rho GTPase activating protein 27 (Arhgap27), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Arhgap27
• Synonyms: 2310069I04Rik; 5730442P18Rik; Gm21552; Sh3d20
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229081 representing NM_001205236
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGGACGTCGAGGGGGACGTGTACGTGCTGGTGGAACACCCCTTCGAGTACACCGGCAAAGACG
  GGCGCCGCATCGCCATCCAACCGAACGAGCGTTACCGACTGCTGCGCCGCAGCACCGAGCACTGGTGGCA
  CGTGCGGCGGGAGCCCGGGGGTCGCCCCTTCTACCTCCCCGCGCAGTACGTACGCGAGCTGCCCGCGCTC
  GGCGACCCTGCCCCGGCCCCGCAGCCTTCCGTACCGCAGCAGCGCCCCGCAGTCCCGGAACCTCTGGCCT
  ACGACTACCGCTTCGTGAGCACCCCGGTGGGCGCCGATGGGTCCTCTGCCGAGCCCCGGGGCCGCGCCAG
  CTCCCTGTGTGGCCCTGCACGACAGCGCACCGGTGGCCAGCGCAACAGCCTGGCTCCGGGAGGGCCCGCC
  TGCCTTTACGTGCGGCCCGCGGCGCCTGTGCGACCGGCGCAGTCCCTGGATGACTTGGCGCGCGGCGGTA
  CCGCGCCCCCTGCCGGCCTCCTCGGCAGCGCGGGCCACTTCAAGGCCTCCAGCGTGGCGGGCTCCTGGGT
  TTGTCCCCGGCCCCTGGCGCCTAGCGACTCGGAGAATGTCTACGAGGCCATTCCGGACTTGCGCTGCCCT
  CCGCGGGCAGAGAGCCCAAAGCAGGTGGACGACCCCCCGGAACCAGTGTATGCCAATGTAGAGAGGCAAC
  CTCGGGCCACGTCGCCTCGCTCAGCTGCAGCTCCTCCTCGGCTCAGCCCAGTGTGGGAGACACACACAGA
  CACGGGCACTGGGCGCCCCTACTACTACAACCCCGACACGGGCGTGACCACCTGGGAGTCACCCTTTGAA
  ACACCTGAAGGCACCACCAGCCCGGCCACCTCCCGGGCCTCTGTGGGTAGCGGGGAGAGCCTGGAGACAG
  AGTGGGGCCAGTACTGGGATGAGGAGAGCCGCAGGGTCTTCTTCTACAACCCCTTGACCGGTGAGACGGC
  CTGGGAGGACGAGACCGAGGAGCTGGAGGAAGATCACCAGGAACAACTGGAAATGCAACCCAGCCTGAGC
  CCGCGAAGCCCCGGGCAGCAGAGGCCCCCAACTCCTGAAACAGACTACCCCGAATTACTGGCCAGTTACC
  CTGAAGAGGACTACTCTCCTGTGGGCTCCTTCAGTGATCCGGGCCCCGCTTCTCCTTTGGTTGCACCCCC
  TGGCTGGTCCTGTCAAATTACCCCAGACAAACAGATGCTCTACACCAACCAGTTCACTCAAGAGCAGTGG
  GTAAGGTTGGAGGACCAGCACGGGAAGCCATATTTCTACAACCCAGAGGACTCCTCTGTTCAGTGGGAGC
  TACCCCAGGTCCCGATCCCTGCCCCCCGAAGTGTCCGCAAATCCAGCCAGGATAGCGACACTCCAGCCCA
  GGCTAGCCCTCCAGAGGAGAAGATCAAGACTCTGGACAAGGCAGGAGTCCTCCATCGTACCAAGACGGTA
  GACAAGGGGAAGAGACTTCGGAAGAAACACTGGAGCACCTCCTGGACAGTGTTGGAGGGCGGGGTCCTGA
  CCTTCTTCAAGGACTCAAAGACCTCAGCTGCAGGCGGCCTGAGGCAGCCTTCCAAGCTCTCCACCCCTGA
  ATACACAGTCGAACTGAAGGGGGCCTCTCTCTCTTGGGCCCCCAAAGACAAATCCAGCAAGAAGAATGTG
  TTAGAGCTTCGAAGCCGAGATGGCTCGGAGTACCTGATCCAGCACGACTCCGAAGCCATCATCAGCACCT
  GGCACAAAGCCATTGCCGAAGGCATCTCAGAGCTGTCTGCAGACCTGCTCCAAGGGGAGGAAGGTGAGCC
  CAGCAGTGCTGATTTCGGGTCCAGTGAGCGCCTCGGAAGCTGGCGGGAGGAGGATGTACGGCAGAACGCA
  GCCTCACCCTCGCTCAGCCCCGGAGGCCTGGAAAGCGACTTGAGCAGAGTCCGGCACAAGCTCCGTAAAT
  TCCTACAGAGACGACCCACTCTGCAGTCTTTGCGGGACAAGGGCTACATCAAAGACCAGGTGTTTGGATG
  CGCGCTGGCTCAACTGTGTGAGCGCGAGAGGAGCCCTGTGCCACGCTTCGTGCAGCAATGCATCCGCACC
  GTCGAGGCCCGGGGGCTGGATATTGATGGGCTGTACCGCATCAGTGGGAACCTGGCTACCATCCAGAAGC
  TGCGCTATAAGGTGGATCACGATGAACGCTTGGACTTGGACGACGGGCGCTGGGAAGACGTCCACGTGAT
  CACGGGTGCCCTGAAGCTCTTCTTCCGAGAGCTGCCGGAACCTCTCTTCCCCTTCTCGCACTTCCACCAG
  TTCATAGCAGCCATTAAGCTGCAGGACCCAGCCCAGCGCAGCCGCTGCGTGCGTGACCTGGTGCGCACGC
  TGCCAGCTCCCAACCAGGACACGCTCCGACTGCTCATCCAGCATCTCTGCCGGGTCATCGAGCATGGCGA
  GCAGAACCGTATGACTGTGCAGAATGTGGCCATAGTGTTCGGACCCACGCTGCTGCGGCCAGAGATGGAG
  GAGGCCAGCATGCCCATGACCATGGTGTTCCAGAACCAGGTGGTGGAGCTCATCCTACATCAGTGCGCGG
  ACATCTTCCCACCGCACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001205236
• Insert Size: 2610 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001205236.1, NP_001192165.1
• RefSeq Size: 4559 bp
• RefSeq ORF: 2610 bp
• Locus ID: 544817
• UniProt ID: A2AB59
• Cytogenetics: 11 E1
• Gene Summary: Rho GTPase-activating protein which may be involved in clathrin-mediated endocytosis. GTPase activators for the Rho-type GTPases act by converting them to an inactive GDP-bound state. Has activity toward CDC42 and RAC1 (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.