Cd22 (NM_009845) Mouse Untagged Clone

CAT#: MC229078

Cd22 (untagged) - Mouse CD22 antigen (Cd22), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cd22
• Synonyms: A530093D23; Lyb-8; Lyb8
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229078 representing NM_009845
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGCGTCCATTACCTGTGGCTCCTCTTGATCCTAGGACACGTGGCTTCGGCTCGGTACAGCTCAGCAA
  ATGATTGGACCGTTGACCATCCCCAAACCCTCTTTGCCTGGGAGGGAGCCTGCATCAGGATTCCTTGCAA
  GTACAAAACTCCACTACCCAAGGCACGTCTGGACAACATCCTCCTTTTTCAGAACTATGAGTTTGACAAG
  GCCACCAAGAAATTCACAGGAACTGTCCTGTACAACGCCACAAAGACTGAGAAGGACCCAGAGTCTGAGC
  TGTACCTTTCTAAGCAAGGGAGAGTAACATTTCTGGGGAACAGAATAGACAATTGTACCCTGAAAATCCA
  CCCGATACGTGCCAATGACAGTGGGAATCTGGGGTTGAGGATGACCGCAGGGACTGAACGATGGATGGAG
  CCCATTCACCTCAATGTCTCGGAGAAACCGTTCCAACCTTACATCCAGATGCCATCAGAAATCCGGGAAT
  CCCAAAGTGTCACTCTGACCTGTGGACTGAATTTCTCCTGCTTTGGGTATGACATCCTCTTGAAGTGGTT
  CCTAGAAGATTCTGAGATCACCTCCATCACCTCTTCTGTCACCTCCATCACCTCTTCTGTCACCTCCTCC
  ATTAAGAATGTCTATACAGAGAGCAAGCTCACCTTCCAACCAAAGTGGACGGACCACGGAAAGAGTGTGA
  AGTGCCAAGTCCAGCACTCCTCCAAAGTGCTCTCAGAGCGCACTGTGCGTCTGGATGTTAAGTATACCCC
  GAAGCTGGAGATCAAGGTCAATCCCACAGAGGTGGAAAAGAACAATTCTGTGACCATGACATGCCGGGTT
  AACAGCAGCAACCCGAAACTCAGGACCGTGGCGGTGTCTTGGTTCAAGGATGGGCGCCCCCTAGAGGATC
  AGGAACTGGAACAGGAACAACAGATGTCCAAGCTAATTCTGCATTCAGTGACCAAGGACATGAGAGGGAA
  ATACCGGTGCCAGGCTTCCAACGACATAGGCCCAGGAGAGTCGGAAGAAGTGGAACTCACGGTGCACTAT
  GCTCCAGAACCCTCCAGGGTTCACATCTACCCTTCCCCCGCTGAAGAGGGACAGTCAGTAGAGCTGATTT
  GTGAGTCACTGGCCAGTCCAAGTGCAACAAACTACACCTGGTATCACAACAGGAAACCTATACCTGGAGA
  CACCCAAGAGAAGCTCCGCATCCCTAAAGTCTCCCCGTGGCATGCTGGGAATTACTCTTGCTTGGCAGAG
  AACCGTCTGGGTCATGGAAAGATAGACCAGGAAGCTAAGCTGGATGTCCATTATGCTCCCAAGGCGGTGA
  CCACAGTGATTCAGAGCTTCACACCAATCCTGGAAGGAGACAGTGTGACCCTGGTCTGTAGGTACAACTC
  CAGCAATCCAGACGTCACCTCCTACAGATGGAACCCTCAAGGTTCTGGGAGTGTGCTCAAACCCGGAGTG
  CTGAGGATTCAGAAAGTGACATGGGATTCCATGCCTGTCAGCTGTGCTGCCTGCAACCACAAGTGTTCGT
  GGGCCCTCCCTGTCATCCTGAATGTCCACTACGCCCCCAGAGACGTGAAGGTACTGAAGGTAAGCCCCGC
  ATCAGAGATCCGCGCTGGGCAGCGTGTCCTCCTCCAATGCGACTTCGCAGAGAGCAACCCGGCAGAGGTC
  CGCTTCTTCTGGAAGAAGAATGGGAGTCTCGTGCAGGAAGGGAGGTACCTGAGCTTCGGCTCCGTCTCCC
  CAGAAGATTCTGGAAATTATAACTGCATGGTCAACAACTCCATCGGAGAGACCTTGTCACAGGCCTGGAA
  CCTCCAAGTGCTGTATGCTCCTCGGAGGCTGCGTGTGTCCATCAGCCCTGGGGACCATGTGATGGAGGGG
  AAGAAGGCCACCTTGTCCTGTGAGAGTGATGCCAATCCGCCCATCTCACAGTACACCTGGTTTGACTCCA
  GTGGCCAAGACCTCCACTCCTCAGGCCAGAAACTGAGACTGGAACCCCTGGAGGTCCAACACACGGGTTC
  CTACCGCTGCAAAGGGACCAATGGGATAGGCACAGGAGAGTCACCACCCAGCACCCTCACTGTCTACTAC
  AGTCCAGAGACCATCGGCAAGCGGGTCGCCTTGGGACTAGGCTTCTGCCTGACTATCTGCATCCTGGCCA
  TCTGGGGGATGAAAATCCAGAAAAAATGGAAGCAAAACCGCAGCCAGCAGGGGCTTCAGGAAAATTCCAG
  TGGCCAGAGCTTTTTTGTGAGGAACAAAAAGGCTAGGAGGACCCCTCTCTCAGAAGGCCCCCAATCCCAG
  GGATGCTACAATCCGGCAATGGATGACACCGTTAGTTATGCCATCTTGCGCTTTCCAGAGAGTGACACGC
  ACAACACTGGAGATGCAGGGACCCCAGCAACACAGGCTCCTCCTCCAAACAACAGCGACTCGGTCACTTA
  CTCGGTGATACAGAAGCGGCCTATGGGGGATTATGAGAATGTGAATCCGAGCTGCCCGGAGGATGAGAGC
  ATCCATTACTCAGAGCTGGTTCAGTTTGGGGCTGGTAAGCGGCCCCAGGCAAAGGAAGACGTAGACTATG
  TGACCCTCAAGCACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_009845
• Insert Size: 2607 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009845.3, NP_033975.3
• RefSeq Size: 3972 bp
• RefSeq ORF: 2607 bp
• Locus ID: 12483
• UniProt ID: P35329
• Cytogenetics: 7 19.26 cM
• Gene Summary: Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.