Snap91 (NM_001277982) Mouse Untagged Clone

CAT#: MC229076

Snap91 (untagged) - Mouse synaptosomal-associated protein 91 (Snap91), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Snap91
• Synonyms: 91kDa; AP180; F1-20; mKIAA0656
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229076 representing NM_001277982
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGGGCCAAACGCTCACGGATCGGATCGCCGCCGCTCAGTACAGCGTGACTGGCTCTGCTGTAGCAA
  GAGCAGTCTGCAAAGCCACCACTCATGAGGTGATGGGCCCCAAGAAGAAGCACCTGGACTATTTGATCCA
  GGCTACCAATGAGACCAATGTCAATATCCCTCAGATGGCCGACACCCTCTTTGAGCGGGCGACAAACAGT
  AGCTGGGTGGTGGTATTTAAAGCTTTAGTGACCACACACCATCTCATGGTGCATGGAAATGAGAGATTTA
  TTCAGTATTTGGCCTCTAGGAATACGCTATTCAATCTCAGCAACTTTCTAGATAAAAGTGGATCCCACGG
  TTATGATATGTCTACGTTCATACGGCGTTACAGTAGATACTTGAATGAAAAGGCTTTCTCCTACAGACAG
  ATGGCATTTGACTTTGCCAGAGTGAAGAAAGGGGCTGACGGTGTCATGAGGACGATGGTTCCTGAAAAGC
  TCCTGAAGAGTATGCCAATCCTGCAGGGGCAGATCGATGCACTGCTGGAGTTTGATGTGCATCCAAATGA
  ACTAACCAATGGTGTCATAAATGCTGCATTTATGCTTCTTTTCAAAGATCTTATCAAACTGTTTGCTTGC
  TACAATGACGGCGTCATTAACTTACTTGAAAAATTTTTCGAGATGAAGAAAGGTCAATGCAAAGACGCGC
  TAGAAATTTACAAGCGATTTCTAACTAGAATGACGAGGGTGTCTGAATTCCTCAAGGTCGCCGAGCAAGT
  TGGTATTGATAAAGGTGACATTCCCGACCTCACACAGGCTCCCAGCAGTCTTATGGAGACCCTTGAACAA
  CATCTAAATACCCTAGAAGGAAAGAAACCTGGAAACAATGAAGGATCTGGTGCTCCCTCTCCACTAAGTA
  AGTCTTCTCCAGCCACAACTGTTACATCTCCTAATTCTACACCAGCTAAAACTATCGACACATCCCCGCC
  AGTTGACATATTTGCAACAGCATCCGCGGCTGCCCCAGTCAGCTCTGCTAAGCCATCAAGCGATCTCCTT
  GATCTTCAGCCCGACTTCTCTGGAGCAGCTGCGGGGGCAGCAGCACCTGTAGTGCCTCCTTCTGGGGGTG
  CGACCGCCTGGGGAGACCTTTTGGGAGAGGATTCCTTGGCTGCACTTTCCTCTGTTCCCTGTGAAGCACC
  GATTTCAGACCCATTTGCACCAGAGCCTTCCCCTCCTACTACAACCACTGAGCCTGCTTCAGCCTCTGCC
  TCGACCACCACAGCTGTGACGGCTGTCACTACGGAAGTGGATCTCTTTGGAGATGCCTTTGCAGCTTCTC
  CTGGGGAGGCCCCTGCAGCATCCGAAGGGGCTACCGCACCAGCTACCCCGGCCCCAGTGGCTGCAGCTCT
  TGATGCATGCTCAGGAAATGACCCTTTTGCCCCATCTGAAGGTAGCGCAGAGGCTGCACCTGAGCTGGAC
  CTCTTTGCAATGAAGCCACCTGAGACCAGCGCTCCTGTAGTTACCCCTACAGCTAGCACAGCCCCTCCAG
  TTCCCGCAACTGCTCCTTCTCCTGCTCCCACGGCTGTGGCAGCCACTGCTGCCACCACCACCGCCGCCGC
  TGCAGCTACCACCACTGCCACCACCTCTGCTGCTGCTGCCACCACCGCCGCTGCTCCTCCTGCTCTAGAT
  ATCTTTGGTGATTTGTTTGATTCTGCTCCTGAAGTTGCTGCAGCACCTAAGCCAGACGCGGCTCCTAGCA
  TAGACCTGTTTGGCACAGATGCTTTCTCCTCCCCGCCACGAGGGGCCTCTCCGGTGCCTGAGAGTTCTCT
  CACTGCTGACCTCTTATCTGTGGACGCATTTGCAGCGCCGTCTCCTGCATCCACTGCCTCTCCTGCAAAG
  GCGGAGTCCTCGGGTGTCATAGACCTTTTTGGGGATGCGTTTGGAAGTGGTGCTTCTGAAACCCAGCCAG
  CACCACAGGCTGTTTCTAGTTCATCAGCATCGGCAGATCTACTAGCTGGATTTGGGGGTTCTTTCATGGC
  CCCTTCTACAACGCCAGTGACTCCAGCTCAGAATAACCTGCTGCAACCCAGTTTCGAGGCAGCTTTTGGA
  ACGACGCCTTCGACTTCAAGCAGCAGCTCTTTTGACCCATCAGGCGATCTTCTGATGCCAACCATGGCAC
  CATCCGGGCAGCCTGCCCCTGTCTCAATGGTCCCACCCAGTCCTGCAATGGCAGCCAGCAAAGGCCTCGG
  AAGTGACCTTGACTCGTCTCTGGCCAGTTTAGTAGGCAATCTTGGGATTTCTGGTACCACATCAAAAAAG
  GGAGATCTCCAGTGGAATGCTGGGGAGAAAAAGCTGACTGGTGGAGCCAACTGGCAACCGAAAGTCACTC
  CAGCCACATGGTCAGCGGGTGTTCCCCCGCCTCCTTCAGGGACAGGCATGACCATGATGTCTCAGCAGCC
  AGTCATGTTTGCACAGCCCATGATGAGGCCACCCTTTGGAGCTGCAGCTGTGCCCGGCACACAGCTTTCT
  CCAAGCCCTACACCTGCCACTCAGAGTCCCAAGAAACCTCCAGCCAAGGACCCGTTAGCGGATCTTAACA
  TCAAGGATTTCTTGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001277982
• Insert Size: 2607 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001277982.1, NP_001264911.1
• RefSeq Size: 4157 bp
• RefSeq ORF: 2607 bp
• Locus ID: 20616
• UniProt ID: Q61548
• Cytogenetics: 9 E3.1
• Gene Summary: Adaptins are components of the adaptor complexes which link clathrin to receptors in coated vesicles. Clathrin-associated protein complexes are believed to interact with the cytoplasmic tails of membrane proteins, leading to their selection and concentration. Binding of AP180 to clathrin triskelia induces their assembly into 60-70 nm coats.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) has an alternate splice site and lacks an in-frame exon in the 3' coding region, compared to variant 1. The resulting isoform (3) is shorter and lacks two segments in the C-terminal region, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.