Trpc7 (NM_001302372) Mouse Untagged Clone

CAT#: MC229061

Trpc7 (untagged) - Mouse transient receptor potential cation channel, subfamily C, member 7 (Trpc7), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Trpc7
• Synonyms: TR; TRP7; Trrp8
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229061 representing NM_001302372
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTGGGGAGCAACACCTTCAAAAACATGCAGCGCCGGCACACCACCTTGAGGGAGAAGGGGCGCCGCC
  AGGCCATCCGGGGTCCTGCCTACATGTTCAACGAGAAGGGCACGAGCCTGACCCCTGAGGAGGAGCGCTT
  CCTGGACTCGGCTGAGTATGGCAACATACCAGTGGTCAGAAAAATGCTGGAGGAATCCAAGACCCTCAAT
  TTCAACTGCGTGGACTACATGGGGCAGAACGCGCTGCAGCTGGCCGTGGGCAACGAGCACTTGGAGGTCA
  CTGAACTGTTGCTGAAGAAAGAGAACTTGGCGCGGGTGGGCGACGCGCTGCTGCTGGCCATCAGCAAGGG
  CTATGTGCGCATTGTGGAGGCCATCCTCAGCCATCCGGCCTTCGCGCAGGGCCAGCGCCTGACGCTCAGC
  CCGCTGGAGCAGGAGCTGCGGGATGATGACTTCTATGCCTACGACGAGGATGGCACGCGCTTCTCCCACG
  ACATCACACCCATCATCCTGGCGGCCCACTGCCAGGAGTATGAGATTGTGCATATCTTGCTGCTCAAGGG
  TGCGCGCATTGAGCGGCCCCATGACTACTTCTGCAAGTGCAATGAGTGCACGGAGAAGCAGCGCAAGGAC
  TCCTTCAGTCACTCGCGCTCCCGGATGAATGCCTACAAAGGACTGGCCAGTGCCGCCTACCTGTCCCTAT
  CCAGTGAAGACCCTGTCCTCACTGCGCTGGAGCTAAGCAACGAGTTAGCCCGACTGGCCAACATTGAGAC
  TGAATTTAAGAATGATTACAGGAAGTTATCTATGCAATGCAAGGATTTTGTGGTGGGCGTGCTGGACCTG
  TGCCGAGACACAGAAGAGGTGGAAGCAATTTTAAATGGCGATGTGAACTTGCAAGTCTGGTCCGACCACC
  ACCGTCCAAGTCTGAGCCGAATCAAACTCGCCATTAAGTACGAAGTCAAGAAGTTCGTTGCTCACCCTAA
  CTGTCAGCAGCAATTGCTTACCATGTGGTATGAAAATCTCTCAGGCTTACGGCAACAGTCTATCGCTGTG
  AAATTCCTGGCTGTCTTTGGAGTCTCCATAGGCCTCCCTTTTCTCGCCATAGCCTATTGGATTGCTCCGT
  GCAGCAAGCTAGGACAAACCCTGAGGAGCCCTTTCATGAAGTTTGTGGCCCATGCAGTGTCTTTTACCAT
  CTTCTTGGGACTGCTAGTTGTGAACGCCTCCGACCGGTTTGAGGGCGTTAAGACTCTGCCCAATGAGACC
  TTCACAGACTACCCCAAACAGATCTTCAGAGTGAAAACCACACAGTTCTCCTGGACGGAGATGCTCATCA
  TGAAGTGGGTCTTAGGAATGATCTGGTCCGAATGCAAGGAGATCTGGGAGGAGGGGCCGCGGGAGTACGT
  GCTACACTTGTGGAACCTTCTGGATTTTGGCATGCTGTCCATCTTCGTGGCCTCCTTCACTGCGAGGTTC
  ATGGCTTTCCTCAAGGCCAGCGAGGCCCAGCTGTACGTGGACCAGTACGTGCAGGATGTAACGCTGCACA
  ACGTCTCACTTCCACCGGAAGTGGCCTACTTCACCTACGCCAGGGATAAGTGGTGGCCTTCAGACCCCCA
  GATCATCTCGGAAGGGCTGTACGCCATTGCGGTTGTCCTCAGCTTCTCCCGGATCGCCTACATCCTGCCA
  GCCAATGAGAGCTTCGGACCTTTGCAGATCTCCCTGGGAAGAACTGTGAAAGACATCTTCAAGTTCATGG
  TCATTTTTATCATGGTATTTGTGGCCTTTATGATCGGAATGTTCAACCTGTACTCCTACTACCGAGGTGC
  AAAGTACAACCCAGCGTTTACCACAGTTGAAGAAAGCTTTAAAACCTTGTTTTGGTCCATATTTGGCTTG
  TCTGAGGTCATCTCGGTGGTGCTGAAATACGACCACAAGTTCATCGAGAATATTGGCTACGTGCTGTATG
  GGGTTTATAATGTCACCATGGTGGTTGTACTACTCAACATGCTGATAGCCATGATCAACAACTCCTATCA
  GGAAATCGAGGAAGACGCGGACGTGGAGTGGAAATTTGCTCGAGCGAAGCTCTGGCTTTCTTACTTTGAT
  GAAGGAAGAACTCTCCCTGCTCCCTTTAACCTGGTGCCGAGCCCTAAATCCTTTTATTATCTCATAATGA
  GAATCAAGATGTGCCTCATAGAGCTCTGCCAATCTAAGGCCAAACGCTGTGAAAACGACCTGGAAATGGG
  CATGCTGAACTCCAAGTTCAGGACTCGCTACCAGGCTGGCATGAGGAATTCTGAAAACCTGACAGCCAAT
  AGCACCTTCAGCAAGCCCACCAGATACCAGAAGATCATGAAGCGGCTCATAAAGAGATACGTGCTGAAGG
  CCCAGGTGGACAGAGAGAACGATGAAGTCAATGAAGGTGAACTCAAGGAGATCAAACAAGACATCTCAAG
  CCTTCGCTATGAGCTCCTGGAGGAGAAGTCTCAGGCTACAGGAGAGCTGGCAGACCTGATCCAGCAGCTC
  AGCGAGAAGTTTGGGAAGAATCTGAACAAAGACCACCTGCGGGTGAACCAGGGCAAGGACATTTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001302372
• Insert Size: 2586 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001302372.1, NP_001289301.1
• RefSeq Size: 3547 bp
• RefSeq ORF: 2586 bp
• Locus ID: 26946
• Cytogenetics: 13 B1
• Gene Summary: The protein encoded by this gene is a member of the transient receptor potential channel family of proteins, which form six-transmembrane cation-permeable channels that are calcium permeant. Knock out mice are viable but display a reduction in the gamma wave activity that precedes seizure induction in response to a muscrarinic agonist, suggesting a functional role for this protein in initiation of seizures. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]
  Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region compared to variant 1. This results in a shorter protein (isoform 2) compared to isoform 1.