Akap1 (NM_001042541) Mouse Untagged Clone

CAT#: MC229055

Akap1 (untagged) - Mouse A kinase (PRKA) anchor protein 1 (Akap1), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Akap1
• Synonyms: Akap; AKAP84; AKAP121; C76494; C81186; DAKAP1; S-AKAP84
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229055 representing NM_001042541
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAATCCAGTTGCGTTCGCTCTTCCCCTTGGCGTTGCCCGGAATGCTGGCCCTCCTTGGCTGGTGGT
  GGTTTTTCTCTCGTAAAAAAGATCGGCTCAGCAGCAGTGATAAGCAGGTGGAGACACTGAAGGTTGGCCC
  TGCCATCAAGGACCGACGGCTCAGTGAAGAGGCCTGTCCTGGAGTCCTGTCTGTGGCCCCCACTGTCACA
  CAGCCTCCTGGAAGGGAAGAGCAGCGCTGTGTGGACAAGCCTTCTACAGAGCCCCTGGCCTTGCCGAGGA
  CTCGCCAGGTTCGACGAAGATCAGAGTCCTCAGGCAACCTCCCCAGCGTTGCAGACACGAGGTCGCAGCC
  AGGACCGTGCAGAGATGAGATCGCCAAAGTGGAACTCTCCCTGATGGGGGACAAAGCCAAATCTATTCCT
  CTTGGATGTCCGCTTCTCCCAAAGGATGCGTCCTTCCCCTATGAAGCAGTGGAAAGGTGTAAGCAGGAGT
  CCGCACTGGGCAAGACTCCTGGAAGAGGCTGGCCAAGCCCGTATGCGGCCTCTGGAGAGAAAGCGAGAGA
  GACAGGTGGGACAGAGGGGACTGGAGATGCTGTGTTGGGGGAAAATGTATCTGAGGAAGGCCTATTGTCC
  CAGGAGTGTGTCTCAGAAGTGGAGAAGAGTGAGTTTCCAATCCTGGCCCCCGGGGGAGGTGAGGGAGAAG
  AGGTGAGCCATGGCCCCCCACAGGTAGCTGAACTTTTAAAGAAGGAAGAATATATTGTTGGGAAGTTGCC
  GAGTAGCTTTGTGGAGCCAGTTCACTCAGAGCCGGTTAAGGACGAGGATGCGTTGGAACCCCAGGTCAAA
  GGTAGCAGCAATACTTCGGATAGAGACCTGGCTGGAGAGCTGGACAAAGACGAGACCGTGCCTGAAAATG
  ACCAGATTAAGCAGGCTGCCTTCCAGCTCATCTCCCAGGTGATCTTGGAAGCAACTGAAGAGTTTCGGGC
  CACCACAGTGGGCAAGACTGTGGCACAAGTGCACCCAACCTCGGCCACTCAGCCTAAGGGGAAGGAGGAG
  AGCTGTGTTCCAGCCAGCCAGGAAACTAGCTTGGGACAAGACACCTCAGATCCTGCTTCCACCAGAACAG
  GTGCCACTGCCAGCCCTTCAGCAGAAGCTCTGCCACCAAAGACCTATGTAAGCTGTCTCAGCAGCCCTCT
  GTCAGGCCCCACCAAGGACCAGAAGCCAAAGAACTCTGCACATCACATCTCCCTGGCTCCCTGCCCACCG
  CCAGTCACCCCCCAGAGGCAGTCTCTGGAGGGGGCAAGTAACCCGAGAGGTGATGACAACTTTGTCGCCT
  GTATGGCCAACAACAGCCAGAGTGTCCTTTCAGTTAGCTCCTTGGGGCAGTGCTCAGATCCTGTCAGTAC
  TTCGGGGCTTGAAGACTCTTGCACAGAGACCATCTCAAGCTCCGGAGACAAAGCTATGACCCCACCACTG
  CCAGTCAGTACTCAGCCCTTCAGCAACGGGGTGCTGAAGGAGGAGCTGTCAGACTTAGGGACCGAGGATG
  GATGGACCATGGATACAGAAGCAGATCACTCAGGAGGTTCTGACGGGAACAGTATGGATTCAGTGGATAG
  CTGTTGCGGGCTTACCAAGCCCGATAGCCCCCAGAGTGTCCAGGCAGGCTCCAACCCTAAGAAGGTTGAC
  CTTATCATCTGGGAGATCGAGGTGCCAAAGCACTTAGTTGGTCGACTGATTGGCAAGCAGGGACGGTACG
  TGAGTTTTCTGAAGCAGACATCTGGTGCCAAGATCTACATCTCCACCCTGCCTTACACACAGAACATCCA
  GATCTGCCACATAGAAGGCTCTCAGCACCATGTAGACAAAGCTCTGAACTTGATTGGGAAGAAGTTTAAG
  GAACTGAACCTCACCAATATCTATGCGCCACCACTGCCTTCGCTGGCACTGCCTTCCTTGCCGATGACGT
  CTTGGCTCATGCTGCCTGATGGTATCACTGTGGAAGTCATCGTGGTCAACCAGGTCAATGCTGGGCACCT
  ATTTGTCCAGCAGCACACACACCCCACCTTCCATGCACTGCGCAGTCTGGACCAGCAGATGTACCTCTGT
  TACTCTCAGCCTGGAATCCCCACCTTGCCCACCCCAGTGGAAATCACGGTTATCTGCGCTGCCCCTGGTG
  CGGACGGGGCCTGGTGGCGAGCCCAAGTAGTGGCTTCCTATGAGGAGACCAATGAGGTGGAGATTCGCTA
  CGTGGACTATGGTGGATATAAGAGAGTGAAAGTCGACGTGCTCCGGCAAATTAGGTCTGACTTTGTGACC
  CTGCCATTCCAAGGAGCAGAAGTCCTTCTGGACAGTGTGGTTCCTCTGTCAGATGATGATCATTTTTCAC
  CGGAGGCAGACGCAGCCATGAGTGAGATGACAGGCAATACAGCACTGTTGGCCCAGGTGACAAGCTACAG
  TGCGACTGGCCTTCCTCTGATTCAGCTATGGAGTGTGGTTGGAGATGAAGTGGTGTTGATAAACCGGTCG
  CTGGTGGAGCGAGGCCTTGCACAGTGGGTAGACAGCTACTATGCCAGCCTCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001042541
• Insert Size: 2574 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001042541.1, NP_001036006.1
• RefSeq Size: 3758 bp
• RefSeq ORF: 2574 bp
• Locus ID: 11640
• UniProt ID: O08715
• Cytogenetics: 11 C
• Gene Summary: Differentially targeted protein that binds to type I and II regulatory subunits of protein kinase A. Anchors them to the cytoplasmic face of the mitochondrial outer membrane or allows them to reside in the endoplasmic reticulum. Does not contain the classic KDEL endoplasmic reticulum-targeting sequence. This explains how it is able to switch its localization, either being in the endoplasmic reticulum or in the mitochondria depending on which N-terminal part begins the isoform. The longest N-terminal part only present in isoform 2 and isoform 4 acts as a suppressor of mitochondrial targeting and as an activator of recessive endoplasmic reticulum targeting motif.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) has an alternate 5' UTR exon, compared to variant 1. Variants 1 and 2 encode the same protein.