Ano5 (NM_001271879) Mouse Untagged Clone

CAT#: MC229052

Ano5 (untagged) - Mouse anoctamin 5 (Ano5), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ano5
• Synonyms: 9330162L24; Gdd1; Tmem16e
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229052 representing NM_001271879
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGGAGCAGGAAGGCTTAACAGCCAAAGAAATAGACTATGCTTTCCAACAGAATGAGAACCTGGGCT
  CCAAAGAGACCAGCTTTCTCATCCCTGAAGACTTACAGTCACCGTTCCAGAGAAGTGAGCACAGCAAAGA
  CTCGGTCTTCTTCAGAGATGGAATCCGGCAGATCGACTTTGTCCTGTCCTACGTGGAGGATTTGAAGAAA
  GATGGAGAGCTGAAGGCAGAAAGAAGACGAGAGTTTGAACAAAATCTCAGAAAAACAGGTCTTGACTTGG
  AAACAGAAGACAAATTGAACTCTGAAGATGGGAAAACCTATTTTGTGAAGATTCACGCCCCATGGGAAGT
  CCTGGTCACTTACGCTGAAGTGCTGGGAATCAAGATGCCTATTAAGTTGAGTGACATCCCACGGCCAAAG
  TACCCACCCCTGTCTTACATGCTTGGGGCGGTGAAGCTTCCATCCTCTGTGAAGTATCCTACTCCTGAGT
  ATTTCACTGCCCAGTTCAGCAGACACCGTCAAGAACTCTTCCTCATTGAAGATGAGGCCACTTTCTTTCC
  ATCCTCAACAAGGAACAGAATTGTTTACTATATTCTGTCACGATGCCCTTTTGGAGTGGAAGAGGGGAAG
  AAAAAGATTGGGATCGAGAGACTGCTGAACTCGAACACTTATTTATCTGCCTACCCGCTCCATGATGGCC
  AGTATTGGAAACCATCAAAAACTACTCGCCCCAATGAAAGGTACAACCTTTGCAAGAACTGGGCTCGATT
  TTCCTACTTTTACAAGGAGCAACCTTTTCATTTGATTCGGAATTATTTTGGAGAAAAAATCGGTATCTAT
  TTTGTATTTCTCGGGTATTACACAGAAATGCTGTTGTTTGCGGCTCTGGTCGGCTTAGCCTGCTTCATCT
  ATGGCTTGTTATCAATGGAAAATAACCGAACTAGCACTGAAATCTGTGACCCTGACATCGGAGGTCAGAT
  GATCATGTGCCCACTTTGCGATGAAGTGTGTGATTACTGGAGACTGAACACCACGTGTTTGCATTCAAAG
  TTCTCCCATCTGTTTGATAACGAGTCGACTGTGTTCTTTGCGCTCTTCATGGGGATCTGGGTCACATTGT
  TTCTGGAGTTTTGGAAGCAGCGACAAGCCAGGCTTGAGTACGAATGGGATCTGGTGGACTTTGAAGAAGA
  ACAGCAGCAGCTCCAGCTTCGGCCTGAATTTGAAGCTATGTGTAAACACAAGAAGATGAATCCTGTGACA
  AAGGAAATGGAGCCACACATGCCTCTATGCCATCGCATCCCATGGTACTTTGTGTCAGGGACCACAGTGA
  CCTTTGGGATGGCTCTCCTCCTCAGCAGCATGGTGTCCATCCTCATATACCGCCTGTCAGTCTTTGCCAC
  TTTTGCCAGCTTCATGGAAAGCGAAGCCACCCTGCAGAGCGTGAAGAGCTTCTTTACTCCACAGCTGGCT
  ACGGCTCTCTCTGGATCATGCCTGAACTGCATTGTCATTTTGATACTGAATTTTTTTTATGAGAAGATAT
  CTGCCTGGATTACGAAAATGGGGAAGTTCGTGGGATATCCTGGGAGTTACACATATATGTTCAACATATG
  GAGAAGCGAAGAGTGTGGTCCTGCAGGCTGTCTGATAGAGCTGACCACCCAGCTAACCATCATTATGATT
  GGAAAACAGATTTTTGGAAACATCCATGAAGCCTTTCAACCCTTGATTTTTAATTGGTGGAGACGCCGAA
  GAGCCCGAACCCACTCTGAGAAACTGTACAGTCGTTGGGAGCAAGATCATGACCTTCAGGTTTATGGACA
  TCGTGGGCTGTTTTATGAGTACTTGGAAACAGTTATCCAGTTTGGGTTTGCCACGCTGTTTGTGGCCTCT
  TTTCCTCTGGCACCCCTGTTTGCTCTCATGAACAACATCATGGGAATCCGAGTGGATGCCTGGAAGCTGA
  CAACTCAGTACAGGAGACCTGTCGCAGCGAAAGCCCACTCCATAGGTGTCTGGCAAGACATTCTTTTTGG
  AATGGCCATCGTTTCTGTTGCAACTAATGCCTTCATTGTCTCATTTACATCTGACATCATCCCCCGGTTA
  GTTTACTTCTATGCCTACTCAACGAATTCCACAGAGCCCTTGAGTGGATACGTCAATAACAGCCTGTCAG
  TGTTCCTGATAGCTGACTTTCCCAACCACACAGTGCCCATGGAGAAAAAAGACTTCGTCACCTGCAGGTA
  CAGAGATTATAGGTACCCTCCTGATCATGAGGATAAGTATAGCCATAACATGCAGTTCTGGCATGTTCTT
  GCTGCCAAGATGACCTTCATCATAGTGATGGAACACGTCGTGTTTCTGTTTAAATTTTTATTAGCCTGGC
  TGATACCAGATGTTCCTAAAGATGTTGTGGAGAAAATCAAACGAGAAAAGTTAATGACTATCAAAATTAT
  TCATGACTTTGAGCTCAACAAGTTGAAAGAAAACTTGGATGTTGAATATGGTAACATCATGAAGAATGTT
  TTAGTAGATGAAGACAATTCCCTGAAGGCTAAAACTACAGTCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001271879
• Insert Size: 2565 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001271879.1, NP_001258808.1
• RefSeq Size: 7633 bp
• RefSeq ORF: 2565 bp
• Locus ID: 233246
• UniProt ID: Q75UR0
• Cytogenetics: 7 B4
• Gene Summary: This gene encodes a member of the anoctamin family, which in mammals is comprised of 10 members. Anoctamin proteins are proposed to have eight transmembrane domains with both termini facing the cytoplasm and a C-terminal domain of unknown function. While some members have been characterized as calcium-activated chloride channels, this protein is reported to have little anion conductance activity. Elevated levels of this protein were found in dystrophic mice. In humans, mutations of this gene are associated with with musculoskeletal disorders such as myopathies, muscular dystrophy and gnathodiaphyseal dysplasia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2012]
  Transcript Variant: This variant (2) lacks an alternate in-frame exon and uses an alternate in-frame acceptor splice site in the coding region compared to variant 1. It encodes isoform 2 which is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.