Amot (NM_001290274) Mouse Untagged Clone

CAT#: MC229049

Amot (untagged) - Mouse angiomotin (Amot), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Amot
• Synonyms: CAG-2; D0Kist1; Sii6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229049 representing NM_001290274
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGAAGTTCTGACGATCAGCCAAGTGGAGGAACGACGGTACTGCAACGGCTGCTTCAAGAGCAGCTTC
  GCTATGGCAATCCCAGTGAGAATCGCAGTCTGCTTGCCATACACCAGCAAGCCACAGGGAATAGCTCTCC
  ATTTTCCACGGGCAGTGGGAACCAAGGCCCTCAGAATGATGTATTGAGTTCCCAGGATCACCACCAGCAG
  CAGCTTGTGGCACACCCTGCTCGCCAAGAGCCCCAGGGTCAGGAAATCCAGTCAGAAAACGGCGTCATGG
  AGAAGCAGCTGTCCCCTCGAATGCAGAATAACGAAGAACTCCCAACCTATGAAGAGGCCAAGGTCCAGTC
  CCAGTACTTCAGGGGCCAGCAGCATGCTAGTGTTGGGGCTGCTTTCTATGTCACAGGAGTCACCAACCAG
  AAGATGAGAACCGAGGGACGCCCGTCAGTCCAGCGCCTCACGCCCGGGAAGATGCACCAAGATGAAGGCC
  TTCGAGATCTTAAGCAAGGGCATGTCCGATCCTTGAGTGAAAGACTGATGCAGATGTCATTGGCTACCAG
  TGGAGTTAAAGCCCACCCACCTGTTACCAGTGCTCCTCTCTCCCCACCACAACCCAATGATCTCTACAAG
  AATGCCACCAGTTCCAGTGAATTCTACAAGGCCCAAGGGCCACCTCCAAGCCAACATAGTCTGAAGGGCA
  TGGAACACCGAGGTCCCCCGCCAGAATACCCTTTCAAGGGCGTGCCATCCCAGTCTGTAGTGTGCAAGTC
  CCAAGAGCCAGGGCACTTCTATAGTGAGCATCGCCTGAACCAGCCAGGGAGAACAGAGGGGCAACTGATG
  AGGTATCAGCACCCCCCTGAGTATGGGGCAGCCAGGGCAACACAGGACATCTCCTCGCTGTCTTTGTCAG
  CCAGGAACTCTCAACCTCATAGCCCTACTTCTTCCCTGACAGCTGGGGCTTCCAGCTTGCCCTTGCTGCA
  GTCTCCGCCGTCCACTAGATTGCCTCCTGGTCAACACCTGGTCTCGAACCAAGGTGACCACTCTGCTCAC
  CTGTCAAGGCATCAGCAGCATCTTCTTTCGAGTCAGAGTCATCAGGGGGACCACTACCGTCACGCCCAGG
  CCAGCCTGACTTCAGCGCAGCAGCAGCCAGGAGAAGCCTACTCAGCTATGCCTCGGGCTCAGCAGTCTGC
  TTCTTATCAGCCCATGCCAGCTGACCCTTTTGCTATGGTCTCTAGAGCTCAGCAGATGGTTGAGATCCTG
  TCTGATGAGAATCGCAACTTGCGGCAGGAGTTGGATGGATGCTATGAGAAGGTGGCGAGACTGCAGAAGG
  TGGAGACAGAAATCCAACGGGTCTCAGAGGCTTATGAGAACTTGGTGAAGTCATCTTCCAAAAGAGAGGC
  TCTGGAGAAAGCCATGAGGAACAAGCTGGAGGGCGAGATTAGAAGGATGCATGACTTCAACAGAGATCTG
  AGAGACCGTCTAGAGACTGCCAACAAGCAGCTGGCAGAGAAGGAGTACGAGGGGTCCGAGGACACCAGGA
  AGACCATCTCGCAGCTCTTTGCCAAACATAAAGAAAACCAGCGGGAGAAGGAGAAGCTGGAAGCTGAGCT
  GGCTACTGCCCGTTCTACCAATGAAGACCAAAGGCGACACATTGAAATCCGAGACCAGGCATTGAGCAAT
  GCCCAGGCGAAGGTGGTGAAGCTGGAAGAAGAGCTGAAAAAGAAGCAAGTGTACGTAGATAAGGTGGAGA
  AGATGCAGCAGGCCCTTGTGCAGCTCCAGGCCGCCTGTGAGAAGCGTGAGCAGCTGGAGCACCGGCTCCG
  GACACGTTTGGAGAGGGAACTGGAATCCCTTAGAATCCAGCAGCGCCAGGGCAACTCCCAGCCCACTAAT
  GCTTCAGAATACAATGCCGCTGCACTGATGGAGCTCCTCCGGGAGAAGGAAGAGAGAATTCTGGCCCTGG
  AAGCAGATATGACCAAGTGGGAGCAAAAGTATTTGGAAGAGAATGTGATGAGACACTTTGCTCTGGATGC
  TGCTGCAACTGTGGCTGCACAGAGAGACACAACAGTCATTAGCCACTCTCCTAACACCAGCTATGACACA
  GCCTTAGAGGCCCGCATCCAGAAGGAAGAGGAAGAAATCCTGATGGCCAACAAGCGTTGCTTGGACATGG
  AAGGAAGGATTAAGACCCTCCATGCCCAGATTATTGAAAAGGATGCCATGATCAAGGTGCTGCAGCAGCG
  CTCCAGAAAGGAGCCGAGTAAAACAGAGCAACTCTCATCCATGCGGCCAGCAAAGTCTCTGATGTCCATT
  TCCAATGCTGGATCAGGCCTCCTTGCACACTCCTCTACCTTGACTGGTGCGCCCATTATGGAGGAGAAGC
  GTGATGACAAGAGCTGGAAGGGGAGCCTAGGTAAGGAAAAGGAAAGCAACAGAAGCAAAGGCACAGTGAC
  AGACTTAGAAAGTGTCCTGACTCTGTTACACACAGCAAGGAAAAGGGACAATGGTCCAGGTTCTAGAGAG
  GAGAATTTGGAGTCCCCCCTCTCAATGGAATTAGATTTATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001290274
• Insert Size: 2562 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001290274.1, NP_001277203.1
• RefSeq Size: 2937 bp
• RefSeq ORF: 2562 bp
• Locus ID: 27494
• UniProt ID: Q8VHG2
• Cytogenetics: X F2
• Gene Summary: Plays a central role in tight junction maintenance via the complex formed with ARHGAP17, which acts by regulating the uptake of polarity proteins at tight junctions. Appears to regulate endothelial cell migration and tube formation. May also play a role in the assembly of endothelial cell-cell junctions (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks several exons and it's 3'-terminal exon extends past a splice site that is used in variant 1. The encoded isoform (2) has a shorter and distinct C-terminus, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.