Eps8 (NM_001271587) Mouse Untagged Clone

CAT#: MC229039

Eps8 (untagged) - Mouse epidermal growth factor receptor pathway substrate 8 (Eps8), transcript variant 4

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Eps8
• Synonyms: AW261790
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229039 representing NM_001271587
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCTCTCAGTCTTGCCTTGACAGTTGCTTGGACGAGTTTTACCTCAGCAGTGCAGGAAGAGGCGTGA
  GAGCCGGCATGAATGGTCATATGTCTAACCGCTCCAGTGGGTATGGAGTCTACCCTTCTCAACTGAATGG
  TTACGGATCTTCACCACCCTATTCCCAGATGGACAGAGAACACAGCTCAAGAACAAGTGCAAAGGCCCTT
  TATGAACAAAGGAAGAACTATGCCCGAGACAGTGTCAGCAGTGTGTCGGACGTGTCCCAGTACCGCGTGG
  AACACTTGACCACCTTCGTGCTGGATCGGAAAGATGCAATGATCACTGTCGAGGACGGAATAAGAAAGCT
  GAAGTTGCTGGATGCCAAGGGCAAAGTGTGGACTCAAGATATGATTCTCCAAGTGGATGACCGAGCTGTG
  AGCCTGATTGACTTAGAGTCAAAGAATGAATTGGAGAATTTTCCTCTAAACACAATCTCGCATTGTCAAG
  CAGTGGTACATGCATGCAGCTATGACTCCATTCTCGCCTTGGTATGCAAAGAGCCAACGCAGAGCAAGCC
  AGACCTTCACCTTTTCCAGTGTGATGAGGTTAAGGCAAACCTAATTAGTGAAGATATCGAAAGTGCAATC
  AGTGACAGTAAAGGTGGGAAACAGAAGAGGCGGCCGGAGGCCCTGAGGATGATTGCCAAAGCAGATCCTG
  GCATCCCTCCTCCTCCCAGAGCTCCTGCCCCTGTGCCACCAGGGACTGTCACACAGGTGGACGTTAGGAG
  TCGCGTAGCAGCCTGGTCTGCCTGGGCAGCTGACCAGGGCGACTTCGAGAAGCCCCGGCAGTACCACGAG
  CAAGAAGAGACGCCCGAGATGATGGCAGCCCGGATCGACAGGGATGTGCAAATCTTAAACCATATTTTGG
  ATGACATTGAATTTTTTATCACCAAACTCCAAAAAGCCGCCGAAGCGTTTTCTGAGCTTTCTAAAAGGAA
  GAAAAGTAAGAAAAGTAAAAGGAAAGGACCTGGAGAGGGCGTTTTAACACTGAGGGCAAAACCGCCACCT
  CCTGATGAATTTGTTGACTGTTTCCAGAAGTTTAAACATGGATTCAACCTTCTGGCCAAGTTGAAGTCCC
  ATATCCAGAACCCGAGTGCTTCAGATCTGGTTCATTTTTTGTTTACTCCACTAAATATGGTGGTCCAGGC
  AACAGGTGGCCCCGAACTGGCCAGTTCGGTACTCAGCCCACTGTTGACAAAAGACACAGTTGATTTCTTA
  AACTACACAGCCACTGCGGAGGAACGGAAGCTGTGGATGTCACTGGGAGATAGTTGGGTGAAAGTGAGAG
  CAGAGTGGCCGAAAGAACAGTTCATCCCACCTTACGTCCCGAGGTTCCGCAACGGCTGGGAGCCCCCGAT
  GCTGAACTTCATGGGCGCGCCCACAGAGCAAGACATGTATCAACTGGCCGAGTCCGTGGCCAACGCAGAA
  CACCAGCGCAAACAGGACAGCAAGAGGCTGTCCACAGAGCATTCCAATGTGTCCGACTATCCTCCAGCCG
  ACGGATATGCGTACAGTAGCAGCATGTACCACAGAGGACCACATGCAGACCACGGGGAGGCTGCCATGCC
  TTTCAAGTCAACTCCTAATCACCAAGTAGATAGGAATTATGACGCAGTCAAAACACAACCCAAGAAATAC
  GCCAAATCCAAGTACGACTTTGTGGCGAGGAACAGCAGCGAGCTCTCGGTTATGAAAGATGATGTCTTAG
  AGATACTCGACGATCGAAGGCAGTGGTGGAAAGTCCGGAATGCCAGTGGAGACTCTGGGTTTGTGCCAAA
  TAACATTCTGGATATCATGAGAACTCCGGAATCTGGAGTGGGGCGCGCTGACCCCCCATACACACATACC
  ATACAGAAACAAAGGACGGAATACGGCCTGAGATCAGCTGACACTCCTTCTGCCCCATCACCCCCTCCAA
  CGCCAGCACCCGTTCCGGTCCCCCTTCCACCTTCTGTACCAGCACCCGTTTCTGTGCCCAAGGTCCCAGC
  CAATGTCACCCGCCAGAACAGCAGCTCCAGTGACAGTGGGGGCAGCATTGTGCGGGACAGCCAGAGATAC
  AAACAACTCCCAGTGGACCGAAGGAAGTCCCAGATGGAAGAGGTTCAGGATGAGCTCTTCCAGAGGCTGA
  CCATCGGGCGCAGTGCTGCACAGAGGAAGTTCCACGTGCCACGGCAGAACGTTCCAGTGATCAATATCAC
  TTATGACTCCTCACCGGAAGAAGTAAAGACTTGGCTGCAGTCAAAGGGATTCAACCCCGTGACTGTCAAT
  AGCCTCGGGGTGTTGAACGGAGCACAACTCTTTTCTCTCAACAAAGACGAACTGAGGTCTGTCTGCCCGG
  AAGGTGCCAGAGTCTTTAACCAAATCACTGTTCAGAAAGCTGCTTTGGAGGACAGTAATGGAAGCTCCGA
  GTTACAAGAGATCATGCGGAGACGGCAGGAGAAGATCAGCGCCGCTGCGAGCGACTCGGGAGTGGAGTCT
  TTCGATGAAGGGAGCAGCCACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001271587
• Insert Size: 2544 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001271587.1, NP_001258516.1
• RefSeq Size: 4561 bp
• RefSeq ORF: 2544 bp
• Locus ID: 13860
• UniProt ID: Q08509
• Cytogenetics: 6 66.78 cM
• Gene Summary: Signaling adapter that controls various cellular protrusions by regulating actin cytoskeleton dynamics and architecture. Depending on its association with other signal transducers, can regulate different processes. Together with SOS1 and ABI1, forms a trimeric complex that participates in transduction of signals from Ras to Rac by activating the Rac-specific guanine nucleotide exchange factor (GEF) activity. Acts as a direct regulator of actin dynamics by binding actin filaments and has both barbed-end actin filament capping and actin bundling activities depending on the context. Displays barbed-end actin capping activity when associated with ABI1, thereby regulating actin-based motility process: capping activity is auto-inhibited and inhibition is relieved upon ABI1 interaction. Also shows actin bundling activity when associated with BAIAP2, enhancing BAIAP2-dependent membrane extensions and promoting filopodial protrusions. Involved in the regulation of processes such as axonal filopodia growth, stereocilia length, dendritic cell migration and cancer cell migration and invasion. Acts as a regulator of axonal filopodia formation in neurons: in the absence of neurotrophic factors, negatively regulates axonal filopodia formation via actin-capping activity. In contrast, it is phosphorylated in the presence of BDNF leading to inhibition of its actin-capping activity and stimulation of filopodia formation. Component of a complex with WHRN and MYO15A that localizes at stereocilia tips and is required for elongation of the stereocilia actin core. Indirectly involved in cell cycle progression; its degradation following ubiquitination being required during G2 phase to promote cell shape changes.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (4) contains a distinct 5' UTR and initiates translation at an alternate start codon, compared to variant 1. The resulting isoform (2) has a distinct N-terminus and is longer than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.