Atp6v0a1 (NM_001243051) Mouse Untagged Clone

CAT#: MC229027

Atp6v0a1 (untagged) - Mouse ATPase, H+ transporting, lysosomal V0 subunit A1 (Atp6v0a1), transcript variant 4

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Atp6v0a1
• Synonyms: AA959968; ATP6a1; Atp6n1; Atp6n1a; Atpv0a1; Vpp-1; Vpp1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229027 representing NM_001243051
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGGAGCTTTTCCGGAGTGAGGAGATGACGCTGGCCCAGCTATTTCTACAGTCTGAAGCTGCTTATT
  GCTGTGTCAGTGAATTAGGAGAGCTTGGGAAGGTCCAATTTCGGGATTTAAATCCAGATGTGAATGTTTT
  CCAGAGGAAATTTGTGAATGAAGTTAGAAGATGTGAAGAAATGGATCGAAAACTCCGATTTGTTGAGAAA
  GAGATAAGGAAAGCTAACATCCCAATTATGGACACCGGTGAAAACCCAGAGGTGCCCTTCCCTCGGGACA
  TGATCGACTTAGAGGCCAATTTCGAGAAGATTGAAAATGAACTGAAGGAAATCAACACTAACCAGGAAGC
  TCTGAAGAGAAACTTCCTGGAACTGACTGAATTAAAATTTATCCTGCGAAAAACCCAGCAGTTTTTCGAT
  GAGGCTGAATTGCATCATCAGCAGATGGCGGATCCAGACCTGTTGGAAGAGTCCTCATCACTCTTGGAGC
  CAAACGAGATGGGAAGAGGCGCACCCTTACGACTTGGCTTTGTGGCTGGTGTGATTAACCGGGAGCGGAT
  CCCGACCTTTGAGCGCATGCTTTGGCGAGTGTGCCGAGGGAATGTGTTCCTGAGACAAGCTGAAATCGAA
  AACCCGCTGGAGGATCCTGTGACTGGCGACTACGTGCACAAGTCTGTGTTCATCATTTTTTTCCAAGGCG
  ATCAGCTGAAAAACAGGGTCAAGAAAATCTGTGAAGGGTTCCGAGCCTCCCTCTATCCCTGCCCTGAGAC
  TCCACAGGAAAGGAAAGAAATGGCTTCCGGAGTCAATACCAGGATCGACGACCTCCAAATGGTTCTGAAT
  CAGACGGAGGACCACCGCCAGAGGGTTCTGCAGGCAGCTGCTAAGAACATCCGCGTCTGGTTCATCAAGG
  TGCGGAAGATGAAGGCTATCTACCATACCCTGAACCTGTGCAATATAGACGTGACCCAGAAGTGCCTGAT
  CGCAGAAGTCTGGTGCCCTGTCACTGACCTGGACTCCATCCAGTTTGCACTACGAAGAGGCACGGAACAC
  AGTGGGTCCACTGTCCCCTCCATTCTGAACAGGATGCAGACAAACCAGACACCGCCGACATATAATAAAA
  CCAACAAGTTCACGCACGGCTTCCAGAACATAGTAGATGCTTATGGGATCGGCACTTACCGAGAGATTAA
  CCCAGCTCCGTACACTGTCATCACCTTCCCCTTTCTGTTTGCTGTGATGTTCGGAGATTTTGGGCACGGC
  ATTTTGATGACGCTGTTTGCTGTGTGGATGGTGTTGAGGGAGAGCCGGATCCTCTCCCAGAAGCACGAGA
  ATGAGATGTTTAGCATGGTGTTCAGCGGCCGGTACATTATTCTTCTGATGGGACTGTTCTCCATCTATAC
  TGGACTCATCTACAATGACTGCTTTTCCAAGTCTCTGAATATCTTTGGGTCATCGTGGAGTGTACGGCCA
  ATGTTCACTCAAGGGAACTGGACGGAGGAGACGCTTCTGGGGAGTTCTGTTCTCCAGTTGAACCCCGCTA
  TCCCTGGAGTTTTTGGTGGCCCGTACCCGTTTGGCATTGATCCGATTTGGAACATTGCAACCAACAAGCT
  GACCTTCCTCAACTCCTTCAAGATGAAGATGTCTGTTATCCTCGGCATCATCCACATGCTGTTTGGAGTC
  AGCCTGAGCCTTTTCAACCATATCTATTTCAAGAAGCCCCTGAACATCTACTTTGGCTTTATTCCTGAGA
  TCATCTTCATGTCCTCGTTGTTTGGCTACCTGGTCATCCTTATCTTTTACAAGTGGACAGCCTACGATGC
  CCACTCGTCTAGGAATGCCCCGAGCCTCCTGATCCACTTCATAAACATGTTCCTCTTCTCCTACCCAGAG
  TCTGGTAATGCAATGCTGTACTCTGGACAGAAAGGAATTCAGTGTTTCCTCATCGTGGTGGCGATGCTCT
  GCGTCCCTTGGATGCTTCTGTTTAAGCCGCTGATCCTTCGCCATCAGTACCTGAGGAAGAAGCATTTGGG
  AACTCTCAACTTTGGTGGGATCAGGGTGGGCAACGGACCGACAGAGGAGGATGCTGAAATTATTCAGCAT
  GACCAGCTTTCCACCCATTCAGAGGACGCAGAAGAGTTTGACTTTGGCGACACCATGGTCCACCAGGCCA
  TCCACACCATTGAGTACTGCTTGGGTTGTATCTCCAACACCGCGTCGTACCTGCGACTCTGGGCCCTCAG
  CCTGGCCCACGCACAGCTCTCTGAAGTACTTTGGACCATGGTGATCCACATTGGCCTGCACGTCCGGAGT
  TTGGCCGGGGGTCTAGGGCTGTTTTTCATCTTCGCCGCCTTTGCCACGCTGACCGTGGCTATCCTGCTGA
  TCATGGAGGGCCTCTCGGCCTTCCTCCACGCGCTGCGGTTACACTGGGTGGAGTTCCAGAACAAATTCTA
  CACTGGGACTGGCTTCAAGTTCCTCCCCTTCTCCTTTGAGCACATTCGGGAAGGGAAGTTTGATGAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001243051
• Insert Size: 2520 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001243051.1, NP_001229980.1
• RefSeq Size: 4022 bp
• RefSeq ORF: 2520 bp
• Locus ID: 11975
• UniProt ID: Q9Z1G4
• Cytogenetics: 11 64.04 cM
• Gene Summary: Required for assembly and activity of the vacuolar ATPase. Potential role in differential targeting and regulation of the enzyme for a specific organelle (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (4) uses an alternate splice site and lacks an in-frame exon in the 3' coding region, compared to variant 1. This results in a shorter protein (isoform 4), compared to isoform 1.