Cdc27 (NM_001285989) Mouse Untagged Clone

CAT#: MC229010

Cdc27 (untagged) - Mouse cell division cycle 27 (Cdc27), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cdc27
• Synonyms: AI452358; APC3; BC023187
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229010 representing NM_001285989
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACGGTGCTGCAGGAACCTGTCCAGGCTGCTATATGGCAAGCGCTAAACCACTATGCTTACCGAGATG
  CAGTTTTCCTCGCAGAACGACTATATGCAGAAGTACATTCAGAAGAAGCCTTGTTTTTACTGGCAACCTG
  TTACTACCGCTCAGGAAAGGCTTATAAAGCATATAGACTCTTGAAAGGACACAGTTGTACCACCCCACAG
  TGTAAATACCTGCTTGCAAAATGTTGTGTTGACCTCAGCAAGCTTGCAGAAGGGGAACAGATCTTATCTG
  GTGGAGTGTTTAATAAGCAGAAAAGCCATGACGACCTTGTCACTGAGTTTGGAGATTCAGCTTGCTTCAC
  TCTTTCCTTGTTGGGACATGTGTATTGCAAGACAGATCGGCTTGCCAAAGGGTCAGAATGTTACCAAAAG
  AGCCTTAGTTTAAATCCTTTCCTCTGGTCTCCCTTTGAATCGTTATGTGAAATAGGTGAGAAGCCAGATC
  CTGACCAAACATTTAAATTAACATCTCTACAGAATTTTAGCAGTTGTCTTCCCAACACTTGTACAACTCT
  AGTATCTAATCACAGTTTATCTCACAGACAGCCTGAGACAGTCCTTACAGAAACACCCCAAGACACAATT
  GAATTAAACAGACTGAATTTAGAATCTTCCAATTCAAAGTACTCCTTGAATACAGATTCCTCCGTGTCTT
  ACATTGATTCAACTGTAATTTCACCAGATAACGTCCCCCTTGGACCTGGTACTGCCATATTATCTAAACA
  GGTTCAAAATAAACCAAAAACTGGTCGAAGTTTATTAGGAGGACCAACTGCTCTTAGCCCATTAACCCCA
  AGTTTTGGGATTTTGCCATTAGAAACCCCAAGTCCTGGAGATGGATCCTATTTACAAAACTACACTAATA
  CACCTTCTGTAATTGATGTGGCACCCACCGGAGCACCTACAAAAAAGTCTGTTGCAAGAATGGGCCAAAC
  TGGAACAAAGTCTGTCTTCTCACAGAGTGGAAATAGTCGAGAGGTCACACCAGTACTTGTTGCACAAACA
  CAAAGTTCTGGCCCACAAACAAGTACAACACCTCAGGTATTGAGCCCCACTATCACATCTCCCCCAAACG
  CATTGCCTCGGAGAAGTTCCCGCCTTTTCACTAGTGACAGTTCTACAACCAAGGAGAATAGCAAAAAGTT
  AAAAATGAAGTTTCCACCTAAAATCCCTAACAGAAAAACAAAAAGTAAAACTAATAAAGGAGGACTAACT
  CAGCCCAGCATAAACGATAGTCTGGAAATTACAAAACTGGACTCCTCTATCATTTCAGAAGGGAAAATAA
  CCACAGTCACACCTCAGATCCAGGCATTTAACCTACAAAAGGCAGCAGCAGGTTTGATGAGCCTTCTTCG
  TGAAATGGGAAAAGGTTATTTAGCTTTGTGTTCATACAACTGCAAAGAAGCTATCAATATTTTGAGCCAT
  CTACCTTCTCATCACTACAGTACTGGTTGGGTCCTATGCCAGATTGGACGGGCTTATTTTGAACTTTCAG
  AATACATGCAGGCTGAAAGAATATTCTCAGAGGTTAGAAGGATTGAGAGTTTCAGAGTTGAAGGAATGGA
  GATCTACTCTACAACACTCTGGCATCTTCAGAAAGACGTTGCTCTTTCAGTTCTTTCAAAAGATTTAACA
  GACATGGATAAGAATTCACCAGAGGCCTGGTGTGCTGCAGGGAATTGTTTCAGTCTACAACGAGAGCATG
  ATATAGCAATTAAATTCTTCCAAAGAGCTATCCAGGTCGATCCAAATTACGCTTACGCCTATACTCTATT
  AGGACATGAGTTTGTGTTAACTGAAGAACTAGATAAAGCATTAGCATGTTTTCGAAATGCTATAAGAGTA
  AATCCCAGACATTACAATGCATGGTATGGTTTAGGAATGATTTATTACAAGCAAGAGAAGTTCAGCCTCG
  CAGAAATGCATTTCCAGAAAGCACTTGATATCAACCCTCAGAGTTCAGTTTTACTTTGCCACATTGGAGT
  AGTTCAGCATGCACTAAAGAAGTCTGAGAAGGCTTTGGATACCCTAAACAAAGCCATTGTTATCGATCCC
  AAGAACCCTCTATGCAAATTTCACAGAGCCTCGGTTTTATTTGCAAATGAAAAATACAAGTCTGCTTTAC
  AAGAACTCGAGGAACTGAAGCAGATTGTCCCCAAAGAGTCCCTCGTTTACTTCCTAATAGGAAAGGTTTA
  CAAGAAGTTGGGTCAAACTCATCTCGCCTTGATGAATTTCTCTTGGGCTATGGATTTAGATCCTAAAGGA
  GCCAATAATCAGATTAAAGAGGCAATTGACAAGCGCTACCTTCCAGATGATGAGGAGCCAATAACCCAGG
  AGGAACAGATCATGGGGACAGATGAATCCCAGGAGAGCAGCATGACAGATGCAGATGACACACAACTTCA
  TGCGGCGGAAAGTGACGAATTTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001285989
• Insert Size: 2475 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001285989.1, NP_001272918.1
• RefSeq Size: 5780 bp
• RefSeq ORF: 2475 bp
• Locus ID: 217232
• Cytogenetics: 11 67.79 cM
• Gene Summary: Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) has multiple differences in the coding region but maintains the reading frame, compared to variant 1. This variant encodes isoform 3, which is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.