Rabep1 (NM_001291141) Mouse Untagged Clone

CAT#: MC228998

Rabep1 (untagged) - Mouse rabaptin, RAB GTPase binding effector protein 1 (Rabep1), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rabep1
• Synonyms: Rab5ep; rabaptin-5; rabaptin-5alpha
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228998 representing NM_001291141
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCAGCCGGGCCCGGCTCCCCAGCCTGACGAGGATCTGAAGAGGCAAAATGCAGTATTGCAAGCTG
  CACAGGACGATTTGGGTCACCTTCGGACACAGCTGTGGGAGGCTCAGGCAGAGATGGAGAACATTAAGGC
  AATTGCCACAGTCTCTGAGAATACTAAGCAAGAAGCTATAGATGAAGTTAAAAGGCAATGGAGAGAAGAA
  GTTGCTTCACTTCAGGCTATAATGAAAGAGACAGTCCGTGACTATGAGCATCAGTTTCACCTGAGGCTGG
  AGCAGGAGCGAGCACAGTGGGCACAGTATCGAGAATCTGCAGAGCGGGAAATAGCTGACTTAAGAAGAAG
  GTTGTCTGAAGGTCAAGAGGAAGAAAATTTAGAAAATGAAATGAAAAAGGCCCAAGAGGATGCTGAAAAG
  CTTCGTTCTGTCGTGATGCCCATGGAGAAGGAAATTGCAGCTCTGAAAGATAAACTGACAGAAGCTGAAG
  ACAAGATTAAAGAGCTGGAGGCCTCAAAGGTTAAGGAGCTGAATCATTATCTGGAGGCCGAGAAGTCTTG
  CAGGACTGACCTGGAGATGTACGTAGCTGTTTTGAACACTCAGAAATCTGTGCTACAGGAAGATGCTGAG
  AAATTGAGGAAAGAACTGCATGAAGTTTGCCATCTCTTGGAACAAGAACGACAACAACACAACCAGTTAA
  AGCATACATGGCAGAAGGCCAACGACCAGTTTCTGGAATCTCAGCGTTTGCTGATGAGAGACATGCAGAG
  AATGGAGATTGTGTTAACTTCGGAACAGCTCCGACAAGTTGAAGAACTAAAGAAGAAAGACCAGGAGGAG
  GATGAACAACAAAGGGTCAATAAGAGAAAAGATAACAAGAAAACAGATACCGAAGAAGAAGTAAAAATAC
  CAGTAGTGTGTGCTTTAACTCAAGAAGAATCTTCAACCCCATTATCAAATGAAGAGGAGCATTTAGACAG
  CACCCATGGGTCAGTCCATTCCTTGGATGCAGACTTGATGTTACCATCTGGAGATCCATTTAGTAAATCG
  GACAATGACATGTTTAAAGATGGACTCAGGAGAGCTCAGTCTACAGACAGCTTGGGAACCTCAAGCTCAT
  TGCAATCCAAAGCTTTAGGCTATAACTACAAAGCGAAATCTGCTGGAAATTTGGATGAGTCGGATTTTGG
  ACCACTGGTTGGAGCAGATTCTGTGTCTGAGAACTTTGATACTGTGTCCCTTGGGTCACTGCAGATGCCA
  AGTGGATTTATGTTAACCAAAGATCAAGAAAGAGCAATTAAGGCAATGACACCTGAGCAGGAGGAGACAG
  CATCCCTCCTCTCCAGTGTCACCCAGGGTATGGAGAGTGCATATGTATCTCCCAGTGGTTACCGCTTAGT
  GAGTGAGACGGAATGGAATCTCCTGCAGAAAGAGGTACATAATGCTGGAAATAAGCTTGGTAGACGTTGT
  GATATGTGTTCCAATTATGAAAAACAGTTACAAGGAATTCAGATTCAGGAAGCTGAAACAAGAGACCAGG
  TGAAAAAACTGCAGTTAATGCTCAGGCAAGCTAATGACCAGCTTGAGAAGACGATGAAAGAGAAGCAGGA
  GCTGGAGGACTTCCTCAAGCAGAGTGCGGAGGACTCAAGCCACCAGATATCTGCACTTGTTTTAAGAGCC
  CAAGCCTCTGAGGTCTTACTTGAAGAGTTACAACAGAGCTTTTCCCAAGCAAAGAGGGACGTTCAGGAGC
  AGATGGCGGTGCTGATGCAGTCACGTGAACAGGTTTCAGAAGAGTTGGTGAGGTTACAGAAAGACAACGA
  CAGCCTCCAGGGGAAGCACAGCTTGCATGTGTCATTACAGCTAGCAGAAGACTTCATTCTTCCAGACACT
  GTGGAGGTGCTCCGGGAACTGGTTTTAAAATATCGTGAGAACATTGTTCATGTGCGCACAGCAGCAGACC
  ACATGGAAGAGAAGCTGAAGGCGGAAATACTTTTCCTAAAAGAGCAAATTCAAGCGGAACAGTGTTTAAA
  AGAAAACCTGGAAGAGACACTACAGCTGGAAATAGAAAACTGCAAGGAGGAAATAGCTTCCATTTCTAGT
  CTAAAAGCTGAATTAGAAAGAATAAAGGTTGAAAAGGGACAGTTGGAATCTACATTAAGAGAGAAGTCGC
  AACAACTTGAGAGTCTCCAAGAAATGAAAGTTAATTTAGAGGAACAGTTAAAGAAAGAAACTGCTGCTAA
  GGCTACTGTTGAACAGCTCATGTTTGAAGAGAAGAACAAAGCTCAGAGGTTGCAGACAGAATTAGATGTC
  AGTGAACAAGTACAGAGAGATTTTGTAAAACTTTCTCAGACTCTTCAGGTGCAGTTGGAGAGGATCCGGC
  AAGCAGACTCCTTGGAGAGGATCCGGGCTATTCTGAATGATACCAAACTGACAGACATTAACCAGCTCCC
  TGAGACATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001291141
• Insert Size: 2460 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001291141.1, NP_001278070.1
• RefSeq Size: 5311 bp
• RefSeq ORF: 2460 bp
• Locus ID: 54189
• UniProt ID: O35551
• Cytogenetics: 11 43.21 cM
• Gene Summary: Rab effector protein acting as linker between gamma-adaptin, RAB4A and RAB5A. Involved in endocytic membrane fusion and membrane trafficking of recycling endosomes. Involved in KCNH1 channels trafficking to and from the cell membrane. Stimulates RABGEF1 mediated nucleotide exchange on RAB5A. Mediates the traffic of PKD1:PKD2 complex from the endoplasmic reticulum through the Golgi to the cilium (PubMed:25405894).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an exon in the 5' coding region, compared to variant 1. The encoded isoform (2) is shorter, compared to isoform 1.