Znrf3 (NM_001290501) Mouse Untagged Clone

CAT#: MC228980

Znrf3 (untagged) - Mouse zinc and ring finger 3 (Znrf3), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Znrf3
• Synonyms: Gm1167
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228980 representing NM_001290501
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGCACCCACTAGGCCTATGTAATAACAATGACGAAGAGGACTTGTATGAATATGGCTGGGTAGGAG
  TGGTGAAGCTTGAACAGCCAGAATTGGACCCCAAACCATGTCTCACTGTCCTGGGCAAGGCCAAGAGAGC
  AGTTCAGCGGGGAGCTACTGCAGTTATCTTTGATGTATCTGAAAATCCAGAAGCCATCGACCAGCTAAAC
  CAAGGCTCAGAAGACCCTCTTAAGAGGCCAGTGGTGTATGTGAAGGGTGCAGATGCTATCAAGCTGATGA
  ACATTGTCAACAAGCAGAAAGTAGCTCGAGCAAGGATCCAGCACCTCCCTCCTCGACAACCCACCGAGTA
  CTTTGACATGGGAATTTTCCTGGCCTTCTTCGTCGTGGTCTCCTTGGTCTGCCTCATTCTCCTTGTCAAA
  ATCAAGCTGAAGCAACGTCGTAGTCAGAATTCCATGAACAGGCTGGCTGTGCAGGCTCTGGAGAAGATGG
  AAACCAGAAAGTTCAACTCCAAGAGCAAGGGGCGCCGGGAAGGAAGCTGTGGGGCCCTGGACACACTGAG
  CAGCGGCTCCACATCCGACTGTGCCATCTGTCTGGAGAAGTACATTGACGGAGAGGAGCTTCGGGTCATC
  CCTTGTACTCATCGGTTCCACAGGAAGTGTGTGGATCCATGGCTACTGCAGCACCACACCTGCCCCCACT
  GTCGGCACAACATCATAGAACAAAAGGGAAATCCAGGTGCTGTTTGTGTGGAGACAAGCAACCTTACACG
  TGGTCGGCAGCCAAGAGTGACCCTACCGGTACATTACCCAGGCCGTGTGCATAGGACCAATGCCATCCCA
  GCCTACCCTACTAGGACAAGTATGGACTCCCATGGCAACCCTGTCACACTGCTAACCATGGACCGGCATG
  GGGAGCAGAACCTCTATTCTCCACAAACACCCACCTATGTCCGTGGCTACCCACCCCTGCACCTGGACCA
  CACTCTGGCCCCTCATCGCTGCAGCCTGGAACACCGGGCCTACTCACCAGCCCATCCCTTCCGCAGGCCC
  AAGTTCAGCAGCCGCAGCTTCTCCAAGGCAGCTTGCTTCTCCCAGTATGAGACCATGTACCAACACTACT
  ACTTCCAGGGTCTCAGCTACCCAGAGCAGGAGGGCCAGACAATACCCAGCGTCACACCCAGGGGCCAATC
  CCGTGCCTTCCCACCAAGTGGTGCCAGCAGCCTGCTCTTCCCCACAATGGTACATGTGGCCCCTCCAACC
  CACGTGGAGAGTGGCAGCACATCTAGCTTCAGTTGCTATCATGGGCACCGTTCTGTGTGTAGCGGCTACC
  TGGCTGACTGTCCTGGCAGCGATAGCAGCAGCAACAGCTCTGGCCAGTGCCGCTGCTCTTCCAGTGACTC
  CGTGGTGGACTGCACAGAAGTCAGCAACCAAGGTGTGTATGGGAGCTGTTCCACCTTCCGAAGCTCCCTC
  AGCAGCGACTATGACCCCTTCATCTACCGCAGCCGGGGGCCTGCTGTGCACCTTGAGGGCTCCCCTCCAC
  CAGAGGAGCTCCCAGCAGGACACAGTCAAAGTGCTGGGAGGGGCGAGCCATGGCTAGGCCCTGCCTCTCC
  CTCAGGGGACCAGCTGTCCACCTGCAGCCTGGAGATGAACTACAGCAGCAACTCCTCGCTGGAACCCAGA
  GGGCCCAACAGCTCTACCTCAGAAGTGGGGCTCGAGGTTTCTCCTGGGGCTGCCCTGGACCTCAGGAGGA
  CCTGGAAAGGGGGTCCAGAGGGACCATCATGTGCTTGCTGCTTCGAGCCCCAGCCCTTCCCGCCGGGCTC
  TGGGATAGAAACAAGTGCAGGTGGCAGCAGTTTATTCCTGGGTCCTCGGCTCCTCGAGGACTGCAACCCT
  CCAAGTGGGGAGCCACAGCTGGGGAGCTCCCAGGGCCTGTATGGTCTTCACTCAGACCATTATCCTAGGA
  CAGATGGGGTGAAATATGAGGGCTTGCCCTGCTGCTTCTATGAAGAGAAGCAGGTGGCCCACAGTGCTGG
  CAGGGGCAATGGCTGCTACACTGAGGACTATTCAGTGAGTGTACAGTACACACTCACCGAGGAACCCCCA
  CCCAGCTGTTATGCAGGGCCCCGGGACCTGAGCCAGCGCATCCCCATTATTCCAGAGGATGTAGACTGTG
  ACTTGGGCTTGCCCCAAGACTGCCACGGGATGCACAACCACAGCCCCTGGGGTGGGGCACTGAGCTTGGA
  CGTTCCCCGACTCCACTGGAGTCTAGGCACAACCCGGGAAGAGGAACAGGCTCCATGTTACCAAGCTGAG
  GTGCAGCCTGGCTGCTCTCCAGAGGAGGCAGGTGCTTCCAGGGCTAGCCTCTCCAGTGCCCCCCAGGACA
  CTCAGGAGTCTCATGCCCTGGCTGCTGAGGCTTCAGGTGAGAACAGATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001290501
• Insert Size: 2430 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001290501.1, NP_001277430.1
• RefSeq Size: 7199 bp
• RefSeq ORF: 2430 bp
• Locus ID: 407821
• UniProt ID: Q5SSZ7
• Cytogenetics: 11 A1
• Gene Summary: E3 ubiquitin-protein ligase that acts as a negative regulator of the Wnt signaling pathway by mediating the ubiquitination and subsequent degradation of Wnt receptor complex components Frizzled and LRP6. Acts on both canonical and non-canonical Wnt signaling pathway. Acts as a tumor suppressor in the intestinal stem cell zone by inhibiting the Wnt signaling pathway, thereby resticting the size of the intestinal stem cell zone (PubMed:22575959, PubMed:22895187). Along with RSPO2 and RNF43, constitutes a master switch that governs limb specification (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR and 5' coding sequence, compared to variant 1. The encoded isoform (2) has a shorter and distinct N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.