Unk (NM_001286006) Mouse Untagged Clone

CAT#: MC228952

Unk (untagged) - Mouse unkempt homolog (Drosophila) (Unk), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Unk
• Synonyms: B230379M23Rik; mKIAA1753; Zc3h5; Zc3hdc5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228952 representing NM_001286006
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGAAGGGCCCCGGGCCCGGCGGCTCGGCAGCTTCCTCGGCGCCCCCGGCCGCTACCGCTCAGGTGC
  TGCAGGCACAGCCCGAGAAACCGCAGCACTACACGTACCTGAAGGAATTCCGCACAGAGCAGTGCCCACT
  CTTCGTGCAGCACAAATGCACACAGCATCGGCCCTATACCTGCTTCCACTGGCACTTTGTGAACCAGCGT
  CGCCGCCGTTCCATCCGCCGACGGGATGGCACCTTCAACTACAGTCCAGATGTCTACTGCACGAAGTATG
  ACGAGGCTACAGGCCTCTGCCCCGAGGGCGACGAGTGCCCATTCCTGCACAGAACCACTGGGGACACTGA
  GCGCAGGTATCACCTTCGTTACTATAAAACTGGAATCTGCATCCATGAGACAGACTCAAAAGGCAACTGC
  ACCAAAAATGGCCTGCACTGCGCTTTTGCCCACGGGCCTCATGACCTCCGCTCCCCTGTTTACGACATCA
  GGGAGCTCCAGGCCATGGAGGCCTTGCAGAATGGCCAGACTACAGTAGAGGGCAGCATAGAAGGCCAGTC
  AGCTGGGGCTGCAAGCCATGCCATGATAGAAAAAATCCTCAGTGAGGAACCCCGGTGGCAAGAGACTGCT
  TATGTGCTGGGGAACTATAAGACGGAGCCGTGCAAGAAGCCTCCACGGCTGTGCCGCCAGGGCTATGCCT
  GTCCCTACTACCACAACAGCAAGGACCGGCGTCGGAGTCCCCGGAAGCACAAATACAGGTCGTCTCCGTG
  TCCAAACGTCAAGCATGGGGATGAGTGGGGAGACCCTGGCAAGTGTGAAAACGGAGACGCCTGCCAGTAT
  TGCCACACACGCACAGAGCAGCAGTTCCACCCAGAGATCTACAAGTCCACCAAGTGTAATGACATGCAGC
  AGGCGGGCAGCTGCCCCCGAGGACCTTTCTGCGCCTTCGCCCACATAGAACCGCCACCCTTAAGTGATGA
  TGTGCAGCCTTCCTCAGCTGTGTCCAGCCCTACCCAGCCAGGTCCCGTCTTGTACATGCCATCTGCCGCT
  GGAGACTCGGTCCCTCTCCTCTGTAGGAATAGTGGCCTGGGCAGTCCGTCTCACCTCTGCAGCTCCCCAC
  CAGGCCCCAGCAGGAAGGCCTCAAACCTGGAGGGTCTGGTCTTCCCTGGGGAGTCCAGCCTTGCCCCTGG
  CAGCTACAAGAAAGCTCCTGGCTTCGAAAGGGAAGACCAGGTTGGGGCAGAGTACCTGAAGAATTTCAAG
  TGCCAGGCTAAATTAAAACCCCATTCGCTAGAGCCCAGGAGTCAAGAGCAGCCTCTGCTTCAACCCAAAC
  AGGACGTGCTGGGCATCCTCCCTGTGGGCAGCCCCCTGACTTCCAGCATCTCTTCCAGTATTACCTCCAG
  CTTGGCAGCCACTCCCCCCAGCCCTGCAGGCACCAACAGCACCCCTGGCATGAATGCAAATGCTCTGCCC
  TTCTACCCAACCAGCGACACGGTAGAGTCGGTCATAGAGTCTGCCTTGGATGACCTGGACCTGAATGAGT
  TTGGAGTGGCTGCCCTAGAGAAGACTTTTGATAACAGCGCAGTGCCCCACCCCAGCAGCGTCACAATCGG
  TGGCAGTTTGTTGCAGAGCTCCGCGCCTGTGAACATCCCTGGCTCCCTGGGCAGCTCAGCTTCCTTCCAC
  TCTGCTTCTCCATCCCCTCCCGTCAGCCTCTCCTCACACTTCCTGCAGCAGCCCCAGGGCCACTTGAGTC
  AGTCAGAAAACACGTTTTTGGGGACCTCAGCATCACATGGATCTTTGGGTCTGAACGGGATGAACAGCAG
  CATCTGGGAGCATTTTGCCTCTGGAAGCTTCTCCCCAGGCACTTCCCCTGCCTTCCTATCAGGGCCAGGG
  GCTGCTGAGCTGGCCCGGCTTCGGCAAGAGCTAGATGAAGCTAACGGCACCATCAAGCAGTGGGAGGAGT
  CCTGGAAGCAGGCCAAGCAGGCTTGTGATGCCTGGAAGAAGGAAGCGGAGGAGGCTGGTGAGCGGGCCAG
  TGCAGCCGGAGCAGAGTGTGAGCTGGCCCGGGAGCAGCGGGATGCCCTGGAGCTGCGGGTGAAGAAGCTG
  CAGGAGGAGCTGGAGCGGCTGCACACGGTGCCAGAAGCCCAGACCCTGCCAGCCGCCCCTGACCTGGAGG
  CGCTGTCCCTCTCCACCCTGTACTCCATCCAGAAGCAGCTGCGGGTCCACCTGGAACAAGTGGACAAGGC
  TGTGTTCCACATGCAGTCAGTGAAATGCCTTAAGTGTCAGGAGCAGACCCGGGCAGTGCTGCCGTGCCAA
  CACGCTGTTCTGTGTGAGCTCTGTGCTGAGGGCAGTGAGTGCCCCGTCTGCCAGCCCAGCCGGGCCCATG
  CCCTCCAGTCGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001286006
• Insert Size: 2394 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001286006.1, NP_001272935.1
• RefSeq Size: 3724 bp
• RefSeq ORF: 2394 bp
• Locus ID: 217331
• Cytogenetics: 11 E2
• Gene Summary: Sequence-specific RNA-binding protein which plays an important role in the establishment and maintenance of the early morphology of cortical neurons during embryonic development. Acts as a translation repressor and controls a translationally regulated cell morphology program to ensure proper structuring of the nervous system. Translational control depends on recognition of its binding element within target mRNAs which consists of a mandatory UAG trimer upstream of a U/A-rich motif. Associated with polysomes (PubMed:25737280).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses an alternate in-frame splice site in the coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1.