Ints10 (NM_001293791) Mouse Untagged Clone

CAT#: MC228812

Ints10 (untagged) - Mouse integrator complex subunit 10 (Ints10), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ints10
• Synonyms: 4921521J11Rik; AI462004
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228812 representing NM_001293791
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGGCCCAGGGCGACTGTGAGTTTCTGGTGCAGCGAGCCCGGGAACTAGTGCCCCAGGACCTGTGGG
  CAGCCAAGGCATGGCTGATCACGGCCCGCAGCCTCTACCCGGCCGACTTCAACATCCAGTATGAAATGTA
  CACCATCGAACGGAATGCCGAGAGGACTGCCACCGCAGGGAGGTTGCTGTATGACATGTTTGTGAATTTC
  CCGGACCAGCCGGTGGTATGGAGAGAAATCAGCATCATCACGTCAGCATTGAGGAACGACTCACAAGATA
  AGCAGACCCAGTTCTTACGGAGTTTATTTGAAACCCTTCCCGGTCGGGTCCAGTGTGAAATGTTGTTGAA
  GGTGACAGAGCAATGCTTCAACACGCTGGAACGATCAGAAATGTTGCTGCTGCTGCTGAGGCGTTTCCCT
  GAGACAGTGGTGCAACACGGGGTTGGCCTCGGGGAGGCCCTGCTGGAAGCTGAAACCATTGAAGAACAAG
  ACTCTCCTGTTAACTGCTTTAGAAAGCTATTTGTTTGTGATGTCCTTCCTCTAATAATCAACAACCATGA
  TGTCCGCTTGCCCGCCAATCTCTTATATAAATACTTGAACAAAGCAGCTGAATTTTACATCAACTATGTC
  ACTCGGTCCACACAGTCAGAAAATCAGCATCAAGGTGCACAGGATACATCTGATTTAATGTCGCCTAGCA
  AGCGTAGCTCTCAGAAGTACATAATAGAAGGACTGACTGAGAAATCATCCCACATCGTCGACCCCTGGGA
  GAGGTTGTTTAAGATTCTGAATGTTGTTGGAATGAGATGCGAATGGCAGATGGATAAAGGGAGACGAAGC
  TGCAGTGACCTGCTGCACCGGATGAAGGAGCTCTGCAGATACATGAACAGCTTTGACAGCGAAGCTCACA
  ACAACTATAAAAACCAAGTGCTCTACTCCACGATGCTGGTGTTCTTCAAGAGTGCGTTCCAGTACGTCAG
  CAGCATACAGCCATCTCTCTTCCAAGGTCCTAACGCCCCAAGCCAGGTTCCACTAATTCTTCTGGAAGAT
  GTGGCCAATGTGTATGGTGATGTAGAAATTGATCGCAGTAAACACATACACAAGAAGAGGAAGCTAGCTG
  AGGGAAGAGAAAAAACCATGAGTTCAGATGATGAAGAGTGTTCAGCGAAAGGCAGAAACCGGCACATTGT
  TGTCAGCAAAGCTGACCTCAGCAACTCCATCGAAGTACTGGAGAGCTTCAAACTGGCCAGGGAAAGCTGG
  GAGTTACTCTACTCTCTGGAATTCCTTGACAAAGAATTTACAAGAATTTGCTTGGCTTGGAAGACAGACA
  CCTGGCTGTGGCTAAGAATCTTCCTCACAGATATGATCATCTACCAGGGTCAATATAAGAAGGCCATAGC
  CAGTCTGCATCACTTAGCAGCTCTGCAAGGATCCCTTTCTCAGCCACAGATCACAGGACAGGGGACTTTA
  GAACACCAGAGGGCGCTCATCCAGCTGGCAACGTGTCACTTTGCTCTGGGGGAGTACAGAATGACGTGCG
  AGAAAGTCCTAGATCTGATGTGTTACATGGTGCTCCCCATACAAGATGGCGGGAAGCCACAGGAAGAGCC
  GTCCAAAGTAAAGCCCAAGTGTAGAAAAGGTTTGGATCTGAAGCTTCTGCCCTGTACCAGTAAGGCTATC
  ATGCCATACTGCCTGCACTTGATGCTGGCCTGCTTTAAGCTGAGAGCTTTTACAGACAGCAGAGACGACA
  TGGCACTGGGCCATGTGATTGTACTGCTCCAGCAGGAGTGGCCACGGGGAGAGAACCTTTTCCTGAAAGC
  CATCAGTAAGATTTGCCAGCAAGGAAATTTCCAGTATGAGAACTTCTTCAGCTATGTTACAAACATTGAT
  ATGCTGGAGGAATTTGCTTATTTAAGAACCCAAGAAGGAGGGAAGATTCATCTGGAATTACTCCCAAATC
  AAGGAATGCTGATCAACCATTGCAGACCTTTTGGGTGGTCCCTCCAGATGAGTGGCAGAGATGTTGTGAG
  CAGTGTACCTCTCCTCCCCTTGGGGCACCACACAGTCACTCGAGGCATCACCAAAGGTGTGAAGGAGGAC
  TTCCGCCTGGCCATGGAGCGCCAGGTCTCCCGCTGTGGTGAGAACCTGATGGCGGTACTACATCGCTTCT
  GCATTAATGAGAAGATCTTGCTCCTGCAGACTCTGACCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001293791
• Insert Size: 2211 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001293791.1, NP_001280720.1
• RefSeq Size: 4279 bp
• RefSeq ORF: 2211 bp
• Locus ID: 70885
• UniProt ID: Q8K2A7
• Cytogenetics: 8 B3.3
• Gene Summary: Component of the Integrator (INT) complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes. May be not involved in the recruitment of cytoplasmic dynein to the nuclear envelope by different components of the INT complex.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.