Map4k2 (NM_001291787) Mouse Untagged Clone

CAT#: MC228808

Map4k2 (untagged) - Mouse mitogen-activated protein kinase kinase kinase kinase 2 (Map4k2), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Map4k2
• Synonyms: AI385662; BL44; GCK; Rab8ip
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228808 representing NM_001291787
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGTTCTGTGGAGGAGGGTCACTGCAGGAGATTTACCACGCCACTGGGCCCCTGGAAGAACGGCAGA
  TTGCCTATGTTTGCCGGGAGGCACTGAAGGGGCTCCACCACCTGCATTCTCAGGGGAAAATTCACCGAGA
  CATTAAGGGTGCCAACCTTCTGCTCACTCTGCAGGGTGATGTCAAGCTGGCGGACTTTGGGGTGTCAGGC
  GAGCTGACAGCATCTGTGGCCAAAAGACGTTCTTTCATTGGCACTCCATACTGGATGGCACCAGAGGTTG
  CCGCTGTGGAACGCAAGGGTGGCTACAATGAGCTGTGTGACGTCTGGGCCCTGGGCATCACTGCCATTGA
  GTTGGGCGAGCTGCAACCGCCACTGTTCCACCTGCACCCCATGAGGGCCTTGATGCTCATGTCGAAAAGC
  AGCTTCCAGCCACCCAAGCTAAGAGATAAGACGCGTTGGACCCAGAATTTCCACCACTTCCTCAAGCTGG
  CCCTAACCAAGAACCCCAAGAAGAGGCCTACAGCAGAGAGGCTTCTGCAGCACCCATTCACGACCCAGCA
  TCTCCCTCCGGCCCTCCTCACCCAGCTCCTCGACAAGGCCAGTGACCCCCACCTGGGAACCCTCTCCCCT
  GAAGACAGTGAGCTGGAGACCCATGACATGTTTCCAGATACCATCCATTCCCGCAGCCATCATGGTCCTG
  CTGAGAGGACCCCCTCCGAGATCCAGTTTCACCAAGTGAAATTCGGTGCTCCTCGCCGGAAGGAGACAGA
  TCCTCTCAATGAACCGTGGGAGGAGGAGTGGACGCTCCTGGGAAAAGAGGAACTGAGTGGGAGCCTGCTG
  CAGTCAGTCCAGGAGGCCCTGGAGGAAAGGAGCCTGACTATCCGGCCAGCCTTAGAACTCCAGGAGCTGG
  ACTCCCCGGATGATGCCATCGGAACCATCAAACGGGCTCCATTTTTGGGGCTGCCCCACACTGAGTCAAC
  ATCTGGTGACAATGCCCAGTCCTGCTCCCCAGGTACCCTGTCAGCACCTCCAGCTGGCCCTGGCAGCCCC
  GCATTGCTGCCTACCGCCTGGGCTACTCTGAAGCAACAGGAGGATCGTGAGCGATCATCCTGCCATGGGC
  TTCCTCCCACCCCCAAGGTGCACATGGGTGCCTGCTTCTCCAAGGTCTTCAATGGCTGCCCCCTGCAGAT
  CCATGCTGCTGTCACTTGGGTTCACCCTGTGACTCGAGACCAGTTCCTGGTGGTAGGGGCTGAGGAGGGC
  ATCTATACCCTCAACCTGCACGAACTGCACGAGGACACAATGGAGAAGCTCATCTCCCAGCGCTGCTCCT
  GGCTCTACTGTGTGAACAATGTGTTGCTGTCGCTCTCAGGGAAATCCACACATATCTGGGCGCATGACCT
  CCCGGGCCTGTTTGAGCAGCGAAGGCTGCAGCACCAGGCACCCCTCTCCATCCCTACCAACCGCATCACT
  CAGCGCATCATCCCCAGGCGCTTTGCCCTGTCCACCAAGATTCCTGACACCAAAGGCTGCTTGCAGTGCC
  GTGTGGTTCGAAACCCCTATACCGGCAGTACGTTCCTGCTGGCCGCCCTGCCTGCCAGCCTGCTTCTGCT
  GCAATGGTATGAGCCACTGCAGAAATTCCTGCTGCTGAAGAACTTTTCCAGTCCCTTGCCCAGCCCAGCG
  GGGATGCTGGAGCCGCTGGTGCTAGATGGGAAGGAGCTGCCACAGGTGTGTGTGGGCGCCGAGGGACCCG
  AGGGACCCGGCTGTCGAGTCCTGTTCCATGTCTTGCCCCTGGAAGCTGGCCTGACTCCAGACATCCTCAT
  CCCTCCTGAGGGGATCCCAGGTTCTGCACAGCAGGTGATCCAAGTGGACAGGGACACAGTCCTGGTCAGC
  TTTGAACGCTGTGTGAGAATTGTTAACCTGCAGGGGGAACCCACGGCTGCACTTGCCCCTGAACTAACCT
  TTGACTTCACTATTGAGACTGTGGTGTGTCTGCAGGATAGTGTGCTGGCGTTCTGGAGCCATGGCATGCA
  GGGCCGAAGCCTCGATACCAACGAGGTGACGCAGGAGATAACAGATGAGACCAGGATCTTCCGAGTACTG
  GGGGCTCACAGGGACATCATCCTGGAAAGCATCCCAACTGATAACCCCGGGGCACACAGCAACCTCTACA
  TCCTCACGGGTCACCAGAGCAGCTACTGA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_001291787
• Insert Size: 2199 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001291787.1, NP_001278716.1
• RefSeq Size: 4653 bp
• RefSeq ORF: 2199 bp
• Locus ID: 26412
• UniProt ID: Q61161
• Cytogenetics: 19 A
• Gene Summary: Serine/threonine-protein kinase which acts as an essential component of the MAP kinase signal transduction pathway (PubMed:8643544). Acts as a MAPK kinase kinase kinase (MAP4K) and is an upstream activator of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway and to a lesser extent of the p38 MAPKs signaling pathway (By similarity). Required for the efficient activation of JNKs by TRAF6-dependent stimuli, including pathogen-associated molecular patterns (PAMPs) such as polyinosine-polycytidine (poly(IC)), lipopolysaccharides (LPS), lipid A, peptidoglycan (PGN), or bacterial flagellin (By similarity). To a lesser degree, IL-1 and engagement of CD40 also stimulate MAP4K2-mediated JNKs activation (By similarity). The requirement for MAP4K2/GCK is most pronounced for LPS signaling, and extends to LPS stimulation of c-Jun phosphorylation and induction of IL-8 (By similarity). Enhances MAP3K1 oligomerization, which may relieve N-terminal mediated MAP3K1 autoinhibition and lead to activation following autophosphorylation (By similarity). Mediates also the SAP/JNK signaling pathway and the p38 MAPKs signaling pathway through activation of the MAP3Ks MAP3K10/MLK2 and MAP3K11/MLK3 (By similarity). May play a role in the regulation of vesicle targeting or fusion (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses an alternate splice junction at the 5' end of an exon compared to variant 1. The resulting isoform (2) is shorter at the N-terminus compared to isoform 1.