Ablim1 (NM_001290813) Mouse Untagged Clone

CAT#: MC228798

Ablim1 (untagged) - Mouse actin-binding LIM protein 1 (Ablim1), transcript variant 4

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ablim1
• Synonyms: 2210411C18Rik; 2610209L21Rik; 4833406P10Rik; 9330196J19Rik; AV079770; AW060987
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228798 representing NM_001290813
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCATCCTTGCTTGGGTTAAAGTGTCTGGGGAAATTGTGCAGCTCTGAGATAGGCAAAGTTCCCTCAC
  CAGAGAGAGCCAGCCTCAGGAACTCCCACAGGAGACTGCTTATTGAGGACCTGAGTGTCCCCGAGACTCC
  TGACCCTGCTCATCGCAGGCGTGGCACAGTCATACATCTGGTCTATCTGTATTCTGCTGGCTGTGGTCCA
  CCAGAGCTCAGGTTTAGCAGCTATGATCCATCCGTGGCCCATCCTCAGGACCCTCACCACTCGTCGGAGA
  AACCTGTCATTCACTGCCATAAATGCGGGGAGCCATGCAAGGGCGAAGTGCTACGGGTGCAGACCAAGCA
  TTTCCACATCAAATGCTTCACCTGCAAAGTGTGCGGCTGCGACCTGGCCCAGGGGGGCTTCTTCATAAAG
  AACGGGGACTATCTGTGTACCCTGGACTACCAGAGGATGTATGGGACACGGTGCCACGGCTGTGGGGAGT
  TCGTGGAGGGCGAGGTGGTAACTGCCCTGGGCAAGACCTACCACCCCAACTGCTTCGCCTGTACAATCTG
  CAAGCGCCCATTTCCACCCGGAGACCGAGTCACATTCAACGGGAGAGACTGTCTTTGTCAGCTCTGTGCA
  CAGCCGATGTCTTCCAGCCCTAAAGAAGCCTCCTGCTCCAGCAACTGCGCCGGCTGTGGAAGGGACATTA
  AGAACGGACAGGCGCTGCTAGCTCTGGACAAGCAGTGGCATTTGGGCTGCTTCAAGTGCAAGTCCTGTGG
  GAAGGTCCTCACCGGCGAGTACATCAGCAAGGATGGCTCCCCATACTGTGAGAAAGACTACCAGGGGCTC
  TTTGGGGTGAAGTGTGAAGCCTGTCACCAGTTTATCACAGGAAAAGTCCTGGAGGCAGGCGACAAGCATT
  ATCATCCCAGCTGCGCACGATGCAGCAGATGCAATCAGATGTTCACAGAAGGGGAAGAAATGTATCTGCA
  AGGTTCTACCGTGTGGCATCCCGACTGTAAGCAGTCCACTAAGACAGAGGAGAAGCTGCGGCCCACCAGG
  ACCTCCTCTGAAAGTATCTATTCTAGACCAGGCTCCAGCATCCCTGGTTCACCAGGCCATACTATCTATG
  CAAAAGTAGACAATGAAATCCTGGATTACAAGGATTTAGCAGCCATCCCCAAGGTCAAGGCGATCTATGA
  CATCGAGCGTCCAGATCTCATTACCTATGAGCCTTTCTACACATCAGGCTATGAGGACAAGCAGGAGAGA
  CAGAGCCTTGGAGAGTCTCCAAGGACTTTGTCTCCAACTCCATCTGCAGAAGGTTATCAAGATGTCCGGG
  ATCGGATGATCCACAGGTCCACCAGCCAGGGCTCCATCAACTCCCCTGTGTATAGCCGGCACAGTTACAC
  TCCAACTACGTCTCGCTCGCCCCAGCACTTCCACCGACCTGGCAATGAGCCGTCCAGCGGCCGGAACTCC
  CCTCTCCCCTACCGGCCCGACAGCCGCCCTCTAACTCCAACTTACGCTCAGGCCCCTAAACATTTCCATG
  TTCCAGATCAAGGGATCAACATTTACCGAAAACCACCCATCTACAAACAGCATGGAACTGACCCGAGGCG
  CAGATCAAGTGGCAGAGAGGAAGATGAAGAGGAGCTTTTGAGACGCCGGCAGCTTCAAGAAGAACAGTTG
  ATGAAGCTCAACTCAGGCCTGGGGCAGCTGATCCTGAAGGAAGAGATGGAGAAGGAGAGCAGAGAAAGAG
  CATCGCTGGCCAGTCGCTATGACTCTCCCCTCCACTCAGCTTCCCATGCTCCATCATCTAAAACTTCATC
  TCTCCCTGGTTATGGAAAGAATGGCCTTCACCGGCCAGTTTCCACAGACTTCGCTCAGTACAACAGCTAC
  GGAGACATCAGTGGCGGAGTTCGAGACTACCAGACCCTCCCAGATGGCCACATGCCTGCAGTGAGAATGG
  ACAGAGGAGTATCCATGCCCAACATGTTGGAACCAAAGATATTTCCATATGAGATGCTCATGGTGACCAA
  CAGAGGGCGCAACAAAATCCTGAGAGATGTGGATAGAACCCGGCTAGAGCGCCACTTAGCCCCAGAAGTA
  TTTTGGGAGATCTTTGGGATGTCCATACAGGAATTTGACAAGTTACCTCTTTGGAGACGCAACGACATGA
  AGAAAAAAGCTAAACTCTTCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001290813
• Insert Size: 2193 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001290813.1, NP_001277742.1
• RefSeq Size: 6245 bp
• RefSeq ORF: 2193 bp
• Locus ID: 226251
• UniProt ID: Q8K4G5
• Cytogenetics: 19 52.09 cM
• Gene Summary: May act as scaffold protein (By similarity). May play a role in the development of the retina. Has been suggested to play a role in axon guidance.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (4) lacks four alternate in-frame exons in the 3' coding region, compared to variant 1. The encoded isoform (4) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.