Unkl (NM_001197024) Mouse Untagged Clone

CAT#: MC228789

Unkl (untagged) - Mouse unkempt-like (Drosophila) (Unkl), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Unkl
• Synonyms: 1300004G08Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228789 representing NM_001197024
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGTCGGTTTCGAAAGCGGCGGCGGCGGCGCTGAGCGGGTCCCCCCCGCAGACAGAGAAACCAACCC
  ACTACAGGTATCTAAAGGAGTTTAGGACAGAGCAGTGCTCCCTGTTTTTGCAGCATAAGTGCAGCCAGCA
  CAGGCCGTTCACCTGTTTCCATTGGCATTTCCTAAATCAGCGTCGTCGCAGACCGCTCCGACGACGCGAT
  GGCACCTTCAACTACAGCCCGGACATATACTGCTCCAAGTACGATGAGGCCACCGGCCTGTGTCCCGATG
  GTGATGAATGCCCTTACCTGCATCGAACAACTGGGGACACGGAGCGCAAGTACCACCTGCGCTACTACAA
  GACAGGCACGTGTATTCACGAGACAGATGCACGGGGCCACTGTGTGAAGAACGGGCTGCACTGTGCCTTC
  GCACACGGGCCCCTGGACCTTCGGCCACCTGTGTGTGACATCAGGGAGCTTCAGGCTCAGGAAGCCCTGC
  AGAATGGCCAGCTCAGTGGAGGGGATGGTGTACCTGATCTGCAGCCTGGGGTCTTGGCCAGCCAGGCCAT
  GATCGAGAAGATTCTTGGTGAGGACCCTCGGTGGCAAGACAGTAACTTCGTATTGGGCAGCTATAAGACT
  GAGCAGTGCCCGAAGCCACCACGCCTATGTCGCCAGGGCTACGCCTGTCCACACTACCACAACAGCAGAG
  ACCGCCGTCGGAACCCCAGGAGGTTCCAGTACAGGTCTACGCCCTGTCCTAGTGTGAAGCACGGTGACGA
  GTGGGGCGAGCCCTCACGGTGTGACGGTGGGGACAGCTGTCAGTACTGCCATTCCCGCACGGAGCAGCAG
  TTCCATCCTGAGATCTACAAATCTACAAAATGCAACGATATGCGCCAAACTGGCTACTGCCCTCGGGGCC
  CTTTCTGTGCCTTTGCACACACTGAGAAGAGCCTGGCTATGGTGAACGAGTGGAGCTGCCGTGATCTCAG
  TTCCAACAGCACCTCAGCTTATAGCAGCCAGCCTGGAAGTGCCAAAAGGAAAGACTCCCCCTCTGAAGGA
  AGCCAGAAAGCTACTGAGGACAGCAAGCAGAACCACCTCGCTGTGTTCTCAGTGGCTCACCCACTGGCCC
  ACAGCATAAGCTCCAGTGTGGCATCCAGCCTGGCGTCCAGCACTGGCTCGGGCAGCTCCTCCCCCACCAC
  ACTGCCTACCCTCCCTGCCCGTGCCCTTCCACTGGATCCCGCCGGCAACACTGTGGGGGCAGTCATAGGT
  TCTGCCTTAGATCTTCGTCTTAGTGACATAAACATCGCGTCCTTAGATAAAGACTTAGAAGAACAGGACC
  TTGGATTGACAGGTCCCAGGTCGCTGGCAGGCTCAGCACCCGTCACCATTCCGGGCTCCCTGCCCCGCTC
  GCCATCCTTGCACTCGTCCTCATCCCTATCCACCTCCCCACTCAGCTCGCTCTCCCAGTCCCTGTCGGGG
  CCACTCGTCTCCTCGGCCATGACGCCCCCCCAGCAGCCACCACCCCTCCGGTCGGAGCCGGCTACGCTGG
  GCTCTGCAGCCTCATCTTACAGCTCTTTAGGTCTGAACGGCGTTCCCGGGAGCATCTGGGACTTTGTTTC
  TGGCAGCTTCTCTCCTAGCCCATCCCCCATTCTGAACTCCGGCCCTTCGGCTTCCTCAAGTGCAAGTCCA
  AACAGTGCTGAGCTGGCACGGGTCAGGCGGCAGCTAGACGAGGCCAAGAGGAAGATCAGGCAATGGGAAG
  AATCTTGGCAGCAAGTGAAGCAGGCCTGTGATGCCTGGCAGAGAGAGGCTCAGGAGGCCAAGGAAAGAGC
  CCGTGTGGCAGACAGTGACCGGCAGCTGGCCCTGCAGAGGAAAGAAGAGGTGGAAGCCAAGGTGAAGCAG
  CTGCAGGAAGAATTAGAGGGCCTGGGTTTGTCCTCACTGCCAGGGCTTCAGAGCTTGGGTGACATCAGTG
  ACATCCCTCTCCCCAAGCTGCATTCTCTCCAGAGCAAGCTGCGCTTGGACTTGGAGGCTGTGGATGGGGT
  GATCTTCCAGCTCCGTGCCAAACAGTGTGTGGCCTGCCAGGAGCGGGCCCATGGCACTGTCCTGCGGCCC
  TGCCAGCACCGTGTCTTATGCGAACCATGTGCGGCCAGTACCCCTGAGTGCCCCTACTGCAAGGGCCAGC
  CCCTACCATGGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001197024
• Insert Size: 2184 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001197024.1, NP_001183953.1
• RefSeq Size: 4775 bp
• RefSeq ORF: 2184 bp
• Locus ID: 74154
• UniProt ID: Q5FWH2
• Cytogenetics: 17 A3.3
• Gene Summary: May participate in a protein complex showing an E3 ligase activity regulated by Rac1. Ubiquitination is directed towards itself and possibly other substrates, such as Baf60b/Smarcd2. Intrinsic E3 ligase activity has not been proven.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longest protein (isoform 1).