Cpeb4 (NM_001290676) Mouse Untagged Clone

CAT#: MC228770

Cpeb4 (untagged) - Mouse cytoplasmic polyadenylation element binding protein 4 (Cpeb4), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cpeb4
• Synonyms: Cpe-bp4; Cpeb-4; mCPEB-4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228770 representing NM_001290676
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGGATTACGGGTTTGGAGTGCTAGTGCAAAGCAATACTGGGAATAAATCTGCTTTTCCAGTCCGAT
  TCCATCCACATCTGCAGCCTCCACACCATCACCAAAATGCCACCCCCAACCCTGCTGCTTTTATAAATAA
  TAACACAGCTGCCAATGGCAGCAGTGCTGGGTCAGCCTGGCTCTTTCCTGCCCCGGCTACTCATAACATT
  CAGGATGAGATCTTGGGGTCAGAAAAAGCAAAAAGTCAACAGCAAGAACAACAAGACCCTTTAGAAAAGC
  AACAGCTCTCCCCGAGTCCAGGTCAGGAAGCTGGAATACTGCCTGAAACTGAAAAGGCAAAAGCTGAAGA
  AAATCCAGGGGACAGTTCTTCAGAAAACAGCAACGGAAAAGAAAAACTACGCATTGAATCACCAGTGTTG
  ACAGGGTTTGATTACCAAGAAGCCACCGGTCTCGGGACTTCCACCCAACCCTTGACATCCAGTGCATCGT
  CCCTTACTGGTTTCAGTAACTGGTCAGCAGCGATAGCACCTTCTTCCTCCACTATAATCAATGAAGATGC
  AAGTTTCTTTCACCAGGGAGGGGTCCCTGGCGCTTCAGCTAATAATGGTGCTCTCTTGTTTCAAAATTTT
  CCCCATCATGTCAGCCCTGGCTTTGGTGGTAGCTTCTCCCCTCAGATCGGGCCTCTCTCCCAGCACCATC
  CTCATCACCCCCATTTCCAGCACCATCACAGCCAGCATCAGCAGCAGAGGAGGTCTCCTGCCAGTCCCCA
  CCCCCCACCTTTCACACATAGAAGTGCTGCTTTTAACCAGCTGCCTCATTTGGCGAATAATCTTAACAAA
  CCTCCTTCTCCATGGAGCAGCTACCAGAGTCCTTCTCCAACCCCCTCTTCTTCCTGGAGCCCAGGAGGTG
  GCGGCTACGGTGGCTGGGGAGCATCTCAAGGCCGGGATCACCGCAGAGGGCTGAACGGTGGAATAACACC
  CCTGAACTCAATCTCACCTTTGAAGAAAAATTTCGCAAGCAATCATATTCAGCTCCAGAAGTATGCTCGC
  CCTAGCTCAGCCTTTGCTCCAAAATCCTGGATGGAAGATAGCTTGAACAGGGCTGACAACATTTTTCCTT
  TTCCGGAACGCCCCAGGACGTTTGACATGCACTCACTGGAGAGCTCACTCATTGACATAATGAGAGCTGA
  AAATGATTCCATTAAAGGTCGTCTAAACTATTCATACCCAGGATCCGATAGTTCTCTGCTTATTAATGGT
  CAGTCTTCATTGTTTCCGATGGAAGATGGATTCCTGGATGATGGCCGTGGGGATCAACCTCTTCATAGTG
  GTCTGGGGTCACCTCACTGCTTCACTCACCAGAATGGGGAGAGAGTGGAACGATACTCTCGCAAAGTGTT
  TGTGGGTGGATTGCCTCCTGATATTGATGAAGATGAGATCACAGCTAGTTTCCGTCGCTTTGGCCCTTTG
  ATTGTGGATTGGCCTCATAAGGCAGAGAGCAAATCTTATTTTCCACCAAAAGGCTATGCATTCCTGCTGT
  TTCAAGATGAAAGTTCTGTTCAGGCTCTCATTGATGCATGTATTGAAGAAGATGGAAAACTTTACCTGTG
  TGTATCAAGTCCAACCATCAAGGATAAACCAGTGCAGATCCGGCCCTGGAATCTCAGTGACAGTGACTTT
  GTGATGGATGGCTCACAGCCACTTGACCCACGGAAAACAATATTTGTTGGTGGTGTTCCTCGACCATTAC
  GAGCTGTGGAGCTTGCAATGATAATGGATCGGCTGTATGGAGGCGTCTGCTATGCTGGAATCGATACTGA
  CCCAGAGCTCAAATACCCAAAAGGAGCTGGAAGAGTCGCGTTTTCTAATCAACAGAGTTACATAGCTGCT
  ATCAGTGCCCGCTTTGTTCAGCTGCAGCATGGAGAGATAGATAAACGGGTGGAGGTTAAGCCATATGTCT
  TGGATGACCAGCTGTGTGATGAATGTCAAGGGGCCCGTTGTGGGGGGAAATTTGCTCCATTTTTCTGTGC
  TAATGTTACCTGTCTGCAGTATTACTGTGAATATTGCTGGGCTGCTATTCACTCTCGTGCTGGCAGAGAA
  TTCCACAAGCCCCTGGTGAAGGAAGGTGGTGACCGCCCTCGGCATATTTCATTCCGCTGGAACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001290676
• Insert Size: 2166 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001290676.1, NP_001277605.1
• RefSeq Size: 7631 bp
• RefSeq ORF: 2166 bp
• Locus ID: 67579
• Cytogenetics: 11 A4
• Gene Summary: Sequence-specific RNA-binding protein that binds to the cytoplasmic polyadenylation element (CPE), an uridine-rich sequence element (consensus sequence 5'-UUUUUAU-3') within the mRNA 3' UTR (PubMed:17024188). RNA binding results in a clear conformational change analogous to the Venus fly trap mechanism (By similarity). Regulates activation of unfolded protein response (UPR) in the process of adaptation to ER stress in liver, by maintaining translation of CPE-regulated mRNAs in conditions in which global protein synthesis is inhibited (PubMed:28092655). Required for cell cycle progression, specifically for cytokinesis and chromosomal segregation (By similarity). Plays a role as an oncogene promoting tumor growth and progression by positively regulating translation of t-plasminogen activator/PLAT (PubMed:22138752). Stimulates proliferation of melanocytes (By similarity). In contrast to CPEB1 and CPEB3, does not play role in synaptic plasticity, learning and memory (PubMed:24386439).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an exon in the central coding region, compared to variant 1. The encoded isoform (2) is shorter, compared to isoform 1.