Acsl3 (NM_001033606) Mouse Untagged Clone

CAT#: MC228764

Acsl3 (untagged) - Mouse acyl-CoA synthetase long-chain family member 3 (Acsl3), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Acsl3
• Synonyms: 2610510B12Rik; Acs3; C85929; Facl3; Pro2194
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228764 representing NM_001033606
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATAACCACGTATCTTCAACACCATCAACCATGAAGCTAAAACAAACCATCAACCCCATACTTTTAT
  ATTTCATACATTTTATAATATCACTCTATACTATTTTAACATACATCCCATTTTATTTTTTGTGTGAGTC
  AAAACAAGAGAAACCAAACCAAATTAAAGCAAAACCTGTCAGTTCCAAACCGGACTCTGCATACAGATCT
  ATCAACAGTGTGGATGGCTTGGCTTCAGTGCTGTATCCTGGCTGCGATACACTTGATAAAGTCTTTATGT
  ATGCAAAAAACAAATTTAAAAACAAAAGACTATTGGGAACACGTGAAATTTTAAATGAGGAAGATGAAAT
  ACAGCCAAATGGAAAAATTTTTAAAAAGGTTATTCTGGGGCACTATAATTGGCTTTCCTATGAAGATGTC
  TTCATCCGAGCCCTTGACTTTGGAAATGGGTTACAAATGTTGGGCCAGAAACCAAAGGCCAACATCGCCA
  TCTTCTGTGAGACCAGGGCTGAGTGGATGATCGCTGCACAGGCGTGTTTTATGTATAACTTCCAGCTTGT
  TACACTGTATGCTACTCTGGGAGGTCCAGCCATTGTTCATGGACTGAATGAGACAGAGGTGACCAACATC
  ATTACTAGCAAAGAACTCTTGCAAACAAAGCTGAAGGATATAGTCTCTTTGGTCCCACGTCTGCGGCATA
  TCATTACTGTTGATGGGAAGCCTCCAACCTGGTCTGAGTTCCCCAAAGGTGTCATTGTACACACCATGGC
  TGCAGTGCAGGCTCTAGGAGTGAAGGCCAACGTGGAAAAGAAAGCTCACAGCAAACCACTGCCCTCAGAT
  ATTGCAGTAATCATGTACACAAGTGGGTCCACAGGAATTCCAAAGGGAGTCATGATCTCACACAGCAACA
  TCATTGCTTCTATAACGGGGATGGCGAGAAGGATTCCAAGACTGGGAGAGGAAGATGTGTATATTGGCTA
  CTTGCCCCTGGCACATGTTCTAGAATTAAGTGCTGAGCTTGTGTGTCTTTCTCATGGATGCCGAATCGGC
  TACTCTTCACCACAGACATTAGCAGATCAGTCTTCAAAAATAAAAAAAGGAAGCAAAGGAGACACATCCG
  TGCTGAAGCCAACACTGATGGCAGCTGTTCCGGAAATCATGGATCGGATCTACAAAAATGTCATGAATAA
  AGTGAATGAAATGAGTGCTTTTCAACGAAACTTGTTTATTTTGGCATATAATTATAAGATGGAACAGATT
  TCAAAAGGGTGTAGTACTCCACTGTGTGACCGCTTTGTTTTCCGGAATGTCCGAAGGCTGCTGGGTGGAA
  ATATTCGCCTTTTACTGTGTGGTGGTGCTCCGCTTTCTGCAACAACGCAGCGATTCATGAATATCTGTTT
  CTGCTGTCCCGTTGGTCAGGGGTATGGACTCACAGAATCTACTGGGGCTGGAACAATTACAGAAGTGTGG
  GACTACAATACCGGCAGAGTGGGAGCACCATTAGTTTGCTGTGAAATCAAATTAAAGAACTGGGAGGAAG
  GTGGCTATTTTAATACTGACAAACCACATCCCAGAGGTGAAATTCTTATTGGTGGCCAAAATGTGACAAT
  GGGGTACTACAAAAATGAAGCAAAAACAAAGACAGATTTCTTTGAAGATGAAAATGGACAGCGGTGGCTG
  TGCACTGGAGATATTGGAGAGTTTGACCCTGACGGCTGTCTGAAGATCATTGACCGTAAAAAGGACCTTG
  TGAAACTACAGGCAGGAGAGTACGTTTCTCTCGGGAAAGTAGAGGCAGCTTTGAAGAACCTCCCACTGAT
  AGATAACATTTGTGCGTATGCAAACAGCTACCATTCTTACGTAATTGGGTTTGTTGTGCCAAATCAAAAG
  GAACTTACAGAGCTAGCTAGAACAAAAGGATTTAAAGGAACTTGGGAAGAGCTGTGTAACAGCAGCGAGA
  TGGAAAATGAGGTCCTTAAAGTGCTTTCTGAAGCTGCTATTTCAGCAAGTCTGGAAAAGTTTGAAATTCC
  ACTAAAAATACGTTTGAGCCCTGACCCATGGACTCCCGAAACTGGTCTGGTGACAGATGCCTTCAAGTTG
  AAACGTAAAGAGCTTAAAACACACTACCAGGCAGACATTGAGCGGATGTATGGAAGAAAATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001033606
• Insert Size: 2163 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001033606.2, NP_001028778.2
• RefSeq Size: 3810 bp
• RefSeq ORF: 2163 bp
• Locus ID: 74205
• UniProt ID: Q9CZW4
• Cytogenetics: 1 40.84 cM
• Gene Summary: Acyl-CoA synthetases (ACSL) activates long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. ACSL3 has mainly an anabolic role in energy metabolism (By similarity). Required for the incorporation of fatty acids into phosphatidylcholine, the major phospholipid located on the surface of VLDL (very low density lipoproteins) (By similarity). Mediates hepatic lipogenesis (By similarity). Preferentially uses myristate, laurate, arachidonate and eicosapentaenoate as substrates (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an exon in the 5' UTR, compared to variant 1. Both variants 1 and 2 encode the same isoform (a).