Nsun7 (NM_001302833) Mouse Untagged Clone

CAT#: MC228729

Nsun7 (untagged) - Mouse NOL1/NOP2/Sun domain family, member 7 (Nsun7), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Nsun7
• Synonyms: 4921525L17Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228729 representing NM_001302833
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGGACCCAACGAGTGAAAGGGACCTTTTTGACCAAGAAAACATGGAGGAGATCTCCCAACTCGCTT
  CCCTGGAGATGTCGGGGGATGTAGTCGCCAATACAAACTCTTCAACCGTCGTCCTAGAAAAACCCAGCTA
  CCCAGACTCCGTGTACGTTACGGCAGCGAACATTTTTCAGGGTATTCGGATCCAAAGGTCGCCAGACAAA
  GTCATAATAAATTACGGAAGTGAACCCCTGCAGCCCTCCTCCTCCCGCTCCGAGGATGAATCTTTCCAGC
  GCTTGTCCTATGAGTTGGCTTTCAGTACCCTGAAGTATCAAGATATTTTGGAAAGTATATTAATAGATAG
  CTATATCTTCTCAAGTACCACAATCGCAAGTCAGCTGAACAGTCTTATCATTGTGATGCTGTATGACTTC
  CAAGACAGAAAATTCCAGCCCCGCATCCTTTCCGAGAATGAAGAGACCATACCAGAAGTTCAAGAAGTCG
  AGAACCTTCTAAACGGCTTTAAGACAAAATTGGCTGCAGCCTTGGCGAGATGTCGAATCAAACACGACGC
  CCTCTCGATTTACCATATTCTGCCAGAGACAGTTAGGAAACAGGAACAAAGGGCCTCAACTTTGCCTCTT
  TATGCTTGGATAAATACCAGTAAAATAAGCCTTGAAGAAGTCTATAATAATTTGAGGAGGAAAGGTTACT
  CTAAAGTCAAGTCTATAACGAGTGTCAATGAGAAAGTCTATGCAGTAGACCAGCACTGCTTCAATGTCTT
  AATTTTCCCAGCTCATCTCAAAACAGACCTTTTAAACATAGATCTCATAAAAGACTATAAACTTATTTTC
  CAGGACAAATCTCGAAGTCTTGCCGTCCACTCTGTGAAGGCTTTGATAAACATCGATGATGATGTCCTAA
  TGGTCAACACGGGTTCGTGGTACACAGTTGCCCATATGTCCATCTTAACAAGTGGTCACACTTCGAAAAT
  CTTTGTATGTGGAATACAACAAGAAGAGAAGGATTTTAATGCGAGGAAACTTTTTACAAGAATGGGATGT
  CAAAATATTGAAATACTTCACGAAACATTTCTTAGCATTGAATCCAAAGATCACCGGTTACAGAATGTGA
  AGGTCATTCTGTTGCTACCTCGCTGTTCCAGCCTCGGTGTCAGCAACCCAGTGGAATTCATTCTAAATGA
  ACACGAAGATAAAAGTTTACTTCAAGATCTTTCTCAAGGTGGTCTACCGAAAGATAAACTCGAGACTTTG
  GTTCAGCAGCAGTTTGAACAGCTAACACACGCAATGAAATTTACCAAAGTTCAAGCAATTGTCTATTGCA
  CATGTTCTGTTTCTAAAGAGGAAAATGAAGACGTTGTTGAGAAAGCACTGGAATATCAAAGCTCAGGGGT
  TAAAATGCAACCTTACAGACTCAGTCCTCCTGTTCTCCCACTGTGCACCCTAAAAGAGATAGAACTGTCT
  ATGGATAGATTCTTCAGATTGGAACCATCTGATATGAACAATGGCTGTTTTCTTTCTATCTTAACAAGAG
  AGCGGGACCCATCTGAGACAGTGTCAGTGAAAGACGTTCTGGCCAGGGCAGCAGCCAAAGGTCTACTGGA
  AGGGGTTGAGGTTGGGAAGACGCTCAAAAGGGACAAGAAGAGGAAAAAATCGAAAGCACTGCCATCCAGG
  GCTCCTCACCATGGGGACCCACTTCGCGACCATTTGGCAGTGGATGGTAACGACACCAGCAACGTCCAAA
  TGAAAATCTCCGAGCTCTTGCACCGAGAAAGCAAGATAAGTACTTCAACAAAAATGTCCGCCCCAGCAAA
  GACTGTGTCTCAAGCCGGCACTTCCTCCCAAGTCAGAAAGCCCAGCAAGCCTTTATCGACCCCACTGGTG
  AGGAATTTTTCCAGGCCGGTAGAAAGGCCAACGAACTTTGTGAGAGCGCGGCCAGAAGGCAAAGTGATTC
  CTTTGAAACCAATTGAAATCGTTCTGCCGCCAGTGATATTCCCATTGAGTTCCCAGGGGCCCAGGGTTCA
  GATGCCAGCCACCCATTTTTATTACCGCTTCATTGGATCAAAGGACTCTTCTAGGGAGGACAGGATCAAT
  AATTTCCTGATAAAGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001302833
• Insert Size: 2118 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001302833.1, NP_001289762.1
• RefSeq Size: 2772 bp
• RefSeq ORF: 2118 bp
• Locus ID: 70918
• UniProt ID: Q14AW5
• Cytogenetics: 5 C3.1
• Gene Summary: This gene encodes a member of the NOL1/NOP2/sun domain RNA methyltransferase family. Mice with a mutation in this gene exhibit male sterility due to impaired sperm motility. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Nov 2014]
  Transcript Variant: This variant (1) encodes the longest isoform (1).