Anapc5 (NM_001289519) Mouse Untagged Clone

CAT#: MC228593

Anapc5 (untagged) - Mouse anaphase-promoting complex subunit 5 (Anapc5), transcript variant 5

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Anapc5
• Synonyms: 2510006G12Rik; AA408751; AA536819; AA986414; Anpc5; APC5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228593 representing NM_001289519
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGACCAACGGGGTAGTGCACGCCAACTTGTTTGGCATCAAGGACTGGGTGACGCCCTATAAGATCG
  CCGTCCTGGTGCTGCTCAACGAGATGGGCCGCACGGGCGAGGGCGCCGTCAGCCTCGTGGAGCGGCGGAA
  GCTCAACCAGCTGCTCCTGCCCCTGCTGCAGGGCCCAGATATTACACTGTCAAAGCTGTACAAGCTAATT
  GAAGAATCGTGTCCTCAGCTGGCAAATTCAGTGCAGATCAGAATCAAGCTCATGGCTGAAGGCGAACTGA
  AGGATATGGAACAATTTTTTGATGACCTTTCAGATTCTTTTTCTGGAACTGAACCAGAGGTTCACAAAAC
  GAGTGTAGTAGGTCTGTTCCTGCGCCACATGATCTTGGCCTACAGTAAGCTTTCTTTCAGTCAAGTGTTT
  AAGCTGTACACTGCCCTCCAGCAGTACTTCCAGAACGGGGAGAAAAAGACGGTGGAAGATGCTGATATGG
  ACCGAGAGGATGGAGAGAAACAGATGGAGAAGGAAGAGCTCGACGTGTCCGTGAGAGAAGAGGAAGTATC
  TTGCAGTGGTCCTCTGTCCCAAAAACAAGCGGAATTTTTTCTCTCTCAGCAGGCCGCTTTGTTGAAGAAT
  GATGAGACTAAAGCCCTCACCCCAGCTTCCTTGCAGAAGGAATTGAACAACCTGTTGAAATTTAATCCTG
  ATTTTGCTGAAGCTCATTACCTCAGCTACTTAAACAACCTCCGTGTTCAAGATGTTTTCAGCTCAACACA
  CAGCCTCCTGCATTATTTTGACCGCCTGATTCTCACTGGAGCGGAGGGCAAAAGTAATGGGGAAGAGGGT
  TATGGCCGGAGCCTGAGATACGCTGCTCTCAACCTGGCTGCCCTGCACTGCCGCTTCGGTCACTATCAAC
  AGGCAGAGCTCGCCCTGCAGGAGGCAATTAGGATTGCCCAGGAGTCCAACGATCACGTGTGTCTGCAGCA
  CTGTTTGAGCTGGCTTTATGTGCTGGGGCAGAAGAGAGCCGATAGCTATGTTCTGCTGGAGCACTCTGTG
  AAGAAAGCAGTACATTTTGGGTTACCGTACCTCGCCTCCCTGGGAATACAGTCCCTTGTTCAACAGAGAG
  CTTTTGCTGGGAAGACGGCCAACAAACTGATGGATGCCCTAAAGGACTCTGACCTCCTGCACTGGAAACA
  CAGCCTGTCAGAACTTATCGATATCAGCATTGCACAGAAAACGGCCATCTGGAGGCTGTACGGCCGCAGC
  ACCATGGCACTGCAACAAGCCCAGATGTTGCTGAGCATGAACAGCCTGGAGTCGCTGAATGCGGGTGTGC
  AGCAGAACAATACTGAGTCCTTTGCCGTCGCTCTCTGCCATCTTGCAGAGCTCCATGCAGAACAGGGCTG
  TTTTGCGGCTGCTGGTGAAGTATTAAAGCACTTGAAGGACCGATTTCCACCCAACAGTCAGCACGCCCAG
  TTATGGATGCTGTGTGATCAAAAAATACAGTTTGACAGAGCAATGAATGATGGCAAATTCCATTTGGCTG
  ATTCACTTGTTACAGGAATCACAGCGCTTAATGGCATAGAAGGTGTATACAGGAAAGCAGTCGTACTGCA
  GGCTCAGAACCAAATGACAGAGGCACACAAGCTACTACAGAAGTTGCTGACGTACTGTCAGAAGCTAAAG
  AACACAGAAATGGTCATCAGAGCTGTACTGGCGATCTTCGTCCCCGACCATCGCCATGCCTGTGCTCCTG
  GAAGCTCTGGCCCTCTCCAAAGAATACCGATTGCAGTACTTGGCCTCCGAAACTGTGCTCAACTTGGCTT
  ATGCCCAGCTCATCCTTGGAATCCCGGAACAGGCCTTAACCCTTCTCCACATGGCTATCGAGCCCATCCT
  AGCCGACGGGGCTGTCCTGGACAAAGGTCGTGCCATGTTCTTAGTGTCCAAGTGCCAAGTGGCTTCGGCA
  GCGTCCTATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001289519
• Insert Size: 1971 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001289519.1, NP_001276448.1
• RefSeq Size: 2770 bp
• RefSeq ORF: 1971 bp
• Locus ID: 59008
• Cytogenetics: 5 F
• Gene Summary: Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (5) uses an alternate splice site, which results in a frameshift, compared to variant 1. The encoded isoform (e) has a distinct C-terminus and is shorter than isoform a.