Dync1i2 (NM_001198873) Mouse Untagged Clone

CAT#: MC228578

Dync1i2 (untagged) - Mouse dynein cytoplasmic 1 intermediate chain 2 (Dync1i2), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Dync1i2
• Synonyms: 3110079H08Rik; AW554389; Dncic2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228578 representing NM_001198873
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGGACAAAAGTGATTTAAAAGCTGAGTTGGAACGTAAGAAGCAACGGTTGGCCCAAATCAGAGAAG
  AAAAGAAGAGGAAAGAAGAGGAAAGGAAAAAAAAGGAAACTGATCAGAAGAAGGAAGCTGCTGTTTCTGT
  ACAAGAAGAGTCTGATCTTGAAAAAAAAAGAAGAGAAGCTGAGGCATTGCTTCAAAGCATGGGACTAACT
  ACGGACTCCCCTATTGAGGATTCTAGCTTCAATCAAGTTGACATAAAACTAGTTTTTTCTGAGTACTGGG
  TCCCTCCTCCTATGTCTCCATCCTCCAAGTCGGTGAGCACGCCAAGTGAAGCTGGAAGCCAGGATTCTGG
  AGATGGCGCCGTGGGATCTAGGACGCTGCATTGGGATACAGATCCATCAGTTCTTCAGCTTCACTCAGAT
  TCCGATTTGGGACGAGGACCTATTAAACTTGGAATGGCCAAAATTACTCAAGTTGACTTTCCCCCTCGAG
  AAATCGTCACATACACAAAGGAAACTCAGACCCCGGTTACAGCTCAACCCAAAGAAGATGAGGAGGAGGA
  AGATGATGTAGCAACTCCTAAACCTCCTGTTGAACCTGAAGAAGAGAAAACTTTGAAAAAAGACGAAGAG
  AATGATAGCAAAGCTCCACCTCATGAACTAACTGAAGAAGAAAAGCAACAGATTTTACACTCAGAAGAAT
  TTTTAAGTTTCTTTGACCATTCTACGAGAATTGTAGAAAGAGCCCTTTCTGAGCAGATTAACATCTTCTT
  TGACTACAGTGGGAGAGACTTGGAAGACAAAGAAGGGGAGATTCAAGCAGGTGCTAAGCTGTCATTAAAT
  CGACAGTTCTTTGATGAGCGTTGGTCAAAGCATCGAGTTGTTAGTTGTTTGGATTGGTCATCCCAGTATC
  CAGAATTACTTGTGGCTTCCTATAATAACAATGAAGAGGCTCCTCATGAGCCTGATGGTGTGGCCCTCGT
  GTGGAATATGAAGTACAAAAAAACTACCCCAGAGTATGTGTTCCACTGCCAGTCAGCTGTAATGTCTGCT
  ACATTTGCAAAATTTCATCCGAATCTTGTTGTTGGAGGTACATATTCAGGCCAGATTGTGCTTTGGGATA
  ACCGCAGCAATAAAAGAACTCCTGTCCAGAGAACGCCGCTGTCAGCTGCTGCACACACACACCCTGTCTA
  CTGTGTAAATGTTGTTGGAACACAAAATGCTCACAATCTGATTAGCATCTCTACTGATGGAAAAATTTGT
  TCCTGGAGTTTGGACATGCTTTCCCATCCACAGGATAGCATGGAGTTGGTTCATAAACAGTCAAAGGCAG
  TAGCTGTAACATCTATGTCTTTCCCTGTTGGAGACGTCAACAACTTTGTTGTGGGCAGTGAAGAAGGTTC
  TGTGTATACAGCATGCCGCCATGGCAGCAAAGCTGGAATCAGTGAGATGTTTGAGGGACACCAGGGACCC
  ATCACTGGAATCCACTGTCACGCGGCTGTTGGAGCAGTAGACTTCTCACATCTGTTTGTCACTTCGTCTT
  TTGACTGGACAGTTAAACTTTGGACAACTAAGAATAACAAGCCTTTGTACTCATTTGAAGATAATTCAGA
  CTATGTCTATGATGTTATGTGGTCACCCACCCACCCAGCCCTGTTTGCCTGTGTGGACGGCATGGGGAGA
  CTGGATCTATGGAATCTCAACAATGACACAGAGGTACCAACTGCCAGCATTTCTGTGGAGGGCAACCCTG
  CTCTTAACCGTGTGAGATGGACCCACTCAGGAAGAGAAATTGCCGTGGGTGATTCTGAAGGACAGATTGT
  CATCTATGATGTGGGAGAGCAGATCGCTGTTCCCCGAAATGATGAATGGGCAAGATTTGGCCGAACACTC
  GCAGAAATTAACGCAAACCGAGCTGACGCAGAGGAGGAGGCAGCTACCCGAATCCCTGCCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001198873
• Insert Size: 1953 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001198873.1, NP_001185802.1
• RefSeq Size: 2659 bp
• RefSeq ORF: 1953 bp
• Locus ID: 13427
• Cytogenetics: 2 42.38 cM
• Gene Summary: Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. The intermediate chains mediate the binding of dynein to dynactin via its 150 kDa component (p150-glued) DCNT1. Involved in membrane-transport, such as Golgi apparatus, late endosomes and lysosomes (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2; also called 1a.E) uses an alternate splice acceptor site in the 5' coding region, compared to variant 1. Variant 2 encodes a shorter isoform (2), compared to isoform 1. Variants 2 and 7 encode the same protein.