Foxn1 (NM_001277290) Mouse Untagged Clone

CAT#: MC228572

Foxn1 (untagged) - Mouse forkhead box N1 (Foxn1), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Foxn1
• Synonyms: D11Bhm185e; Fkh19; HFH-11; Hfh11; nu; nude; wh; Whn
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228572 representing NM_001277290
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGTCGCTACTCCCTCCGCAGTCTGACGTCACACTTCCAGGCTCCACCCGACTGGAGGGCGAACCCC
  AAGGGGACCTCATGCAGGCTCCGGGCCTCCCAGACTCCCCTGCCCCACAGAACCATGCTAACTTCAGCTG
  CTCGTCGTTTGTGCCTGACGGCCCTCCAGAGAGGACACCCTCACTGCCCCCACACAGCCCCAGCATCGCA
  TCTCCAGACCCAGAGCAGATCCAGGGCCACTGCACAGCCGGACCCGGCCCGGGCTCCTTCCGCCTTTCTC
  CTTCAGAAAAGTATCCTGGCTTTGGCTTTGAGGAGGGCCCAGCAGGCAGCCCAGGGCGCTTCCTCAAGGG
  CAACCACATGCCTTTCCACCCCTACAAGAGGCACTTCCATGAGGACATCTTCTCTGAGGCCCAGACGGCC
  ATGGCACTTGATGGACACTCCTTTAAGACTCAGGGGGCACTGGAAGCCTTTGAGGAGATCCCTGTGGACA
  TGGGCGATGCTGAGGCCTTCCTGCCTAGCTTCCCAGCAGAGGCTTGGTGCAATAAACTCCCTTACCCCAG
  CCAGGAACACAACCAAATTCTGCAGGGGTCAGAGGTCAAGGTCAAGCCCCAAGCTCTGGACAGTGGTCCT
  GGGATGTACTGCTACCAGCCTCCCTTGCAACATATGTACTGTTCTTCTCAGCCTGCCTTCCATCAGTACT
  CCCCGGGTGGAGGCAGCTACCCTGTGCCCTACCTGGGCTCACCTCACTATCCCTATCAGAGGATTGCACC
  CCAGGCCAACGCCGAAGGTCACCAGCCACTCTTCCCAAAGCCCATCTACTCTTACAGCATCCTCATCTTC
  ATGGCCCTTAAGAACAGTAAGACCGGAAGCCTTCCAGTCAGTGAAATCTACAATTTCATGACGGAGCACT
  TCCCTTACTTCAAGACTGCTCCTGATGGCTGGAAGAATTCTGTTCGCCATAACCTGTCCCTCAACAAGTG
  CTTTGAGAAGGTGGAGAATAAATCCGGAAGTTCCTCTCGAAAGGGCTGTCTGTGGGCCCTCAATCCTTCC
  AAAATCGACAAGATGCAGGAAGAACTGCAGAAGTGGAAGAGGAAAGACCCCATTGCTGTGCGCAAAAGCA
  TGGCCAAACCAGAAGAGCTGGACAGCCTCATTGGAGACAAAAGGGAAAAACTGGGCTCTCCGCTGCTGGG
  CTGTCCACCCCCTGGGCTGGCAGGCCCAGGTCCCATCCGGCCCATGGCACCATCAGCTGGTCTTTCCCAG
  CCTCTGCACCCAATGCATCCAGCTCCAGGCCCCATGCCTGGCAAGAACCCCCTGCAGGACCTACTGGGTG
  GCCATGCTCCCTCCTGCTATGGGCAGACCTACCCACACCTTTCCCCCAGCCTGGCCCCTTCTGGACACCA
  GCAGCCATTGTTCCCACAGCCAGATGGGCATCTTGAGCTGCAGGCCCAGCCAGGCACCCCCCAGGACTCA
  CCTCTACCTGCCCACACACCACCCAGCCACGGTGCCAAGCTGATGGCTGAGCCTTCCTCAGCCAGGACCA
  TGCACGATACTCTACTGCCAGATGGAGACCTTGGGACTGACCTGGATGCTATCAACCCTTCTCTCACTGA
  CTTCGACTTCCAGGGAAATCTGTGGGAGCAGCTGAAGGATGACAGCTTGGCCCTGGACCCCCTCGTATTG
  GTGACCTCGTCGCCGACGTCATCCTCCATGTTGCCACCCCCACCAGCAGCCCATTGCTTCCCCCCAGGGC
  CTTGTCTGGCAGAAACAGGCAATGAGGCAGGTGAACTGGCACCTCCAGGCAGCGGCGGCTCCGGTGCTCT
  GGGAGACATGCACCTCAGCACTCTCTACTCCGCCTTTGTGGAACTGGAGTCCACGCCCTCCTCAGCAGCT
  GCCGGCCCTGCCGTGTACCTCAGTCCCGGCTCAAAGCCATTGGCTCTGGCTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001277290
• Insert Size: 1944 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001277290.1, NP_001264219.1
• RefSeq Size: 3270 bp
• RefSeq ORF: 1944 bp
• Locus ID: 15218
• Cytogenetics: 11 46.74 cM
• Gene Summary: The protein encoded by this gene is part of the forkhead family or "winged-helix" transcription factors that are important in developmental processes, immune system regulation, metabolism, cancer and aging. This gene family has over 100 members, subdivided into classes (A-Q) based on phylogeny. The encoded protein is proposed to regulate development of the thymus and differentiation of keratinocytes. Mutations in this gene cause severe primary T-cell immunodeficiency and congenital alopecia. In mouse mutations of this gene underlie the phenotype of the nude mouse, which has been widely used as a model system in oncology, immunology, dermatology, and transplantation studies. In humans mutations in this gene have been correlated with T-cell immunodeficiency, the skin disorder congenital alopecia, and nail dystrophy. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Apr 2013]
  Transcript Variant: This variant (2) uses an alternate in-frame acceptor splice site in the 5' coding region compared to variant 1. It encodes isoform 2 which is shorter compared to isoform 1.