Umod (NM_001278605) Mouse Untagged Clone

CAT#: MC228557

Umod (untagged) - Mouse uromodulin (Umod), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Umod
• Synonyms: THP; ureh; Urehd; Urehd1; urehr4; uromu
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228557 representing NM_001278605
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGATCCCTTTGACCTGGATGCTGCTGGTAATGATGGTAACCTCCTGGTTCACTCTGGCTGAAGCCA
  GTAACTCAACAGAAGCGAGACGGTGTTCTGAATGCCACAACAACGCCACCTGCACGGTGGATGGTGTGGT
  CACAACGTGCTCCTGCCAGACCGGCTTCACTGGTGATGGGCTGGTGTGTGAGGACATGGATGAGTGTGCT
  ACCCCATGGACTCACAACTGCTCCAACAGCAGCTGTGTGAACACCCCGGGCTCGTTTAAGTGCTCCTGTC
  AGGATGGTTTTCGTCTGACGCCTGAGCTGAGCTGCACTGATGTGGATGAGTGCTCAGAGCAGGGGCTCAG
  TAACTGTCATGCCCTGGCCACCTGTGTCAACACAGAAGGCGACTACTTGTGCGTGTGTCCCGAGGGCTTT
  ACAGGGGATGGTTGGTACTGTGAGTGCTCCCCAGGCTCCTGTGAGCCAGGACTGGACTGCTTGCCCCAGG
  GCCCGGATGGAAAGCTGGTGTGTCAAGACCCCTGCAATACATATGAGACCCTGACTGAGTACTGGCGCAG
  CACAGAGTATGGTGTGGGCTACTCCTGTGACGCGGGTCTGCACGGCTGGTACCGGTTCACAGGCCAGGGT
  GGCGTTCGCATGGCTGAGACCTGTGTGCCCGTCCTGCGATGCAACACGGCGGCACCCATGTGGCTCAATG
  GCTCTCATCCCTCGAGTAGTGAAGGCATTGTGAGCCGCACGGCCTGTGCACACTGGAGCGACCAATGCTG
  CCGGTGGTCCACAGAGATCCAGGTGAAGGCTTGCCCAGGTGGCTTCTATATTTACAACTTGACAGCGCCC
  CCTGAGTGCAATCTGGCTTACTGCACCGATCCTAGTTCCGTGGAGGGGACTTGCGAAGAATGCAGGGTAG
  ATGAAGATTGCATATCGGATAACGGCAGATGGCGCTGCCAGTGTAAACAGGACTCCAACATCACAGATGT
  CTCCCAATTGGAGTACAGGCTGGAGTGTGGGGCCAATGACATCAAGATGTCCCTCAGAAAGTGCCAGCTA
  CAGAGTTTGGGCTTTATGAATGTCTTCATGTACCTGAATGACAGACAATGCTCAGGCTTCAGTGAGAGTG
  ATGAACGAGACTGGATGTCCATAGTGACCCCTGCCAGGAATGGTCCCTGTGGGACAGTATTGAGGAGAAA
  CGAAACCCATGCCACCTACAGCAACACCCTCTACCTGGCAAATGCGATCATCATTCGGGACATCATCATA
  AGAATGAACTTTGAATGCTCTTACCCTCTGGACATGAAAGTCAGCCTGAAGACCTCCCTACAGCCCATGG
  TCAGTGCCCTGAACATCAGCTTGGGTGGGACAGGCAAGTTCACCGTGCGGATGGCATTGTTCCAGAGCCC
  TACCTACACACAGCCCCACCAAGGTCCTTCTGTGATGCTGTCCACTGAGGCTTTTCTGTATGTGGGCACC
  ATGCTGGATGGGGGTGACTTGTCCCGGTTTGTACTGCTAATGACCAACTGCTATGCCACACCCAGTAGCA
  ACTCCACAGACCCTGTGAAATACTTCATTATCCAGGACAGTTGTCCACGTACAGAAGATACAACCATTCA
  GGTGACAGAGAATGGCGAGTCATCTCAGGCCCGATTTTCTGTTCAGATGTTCCGGTTTGCAGGAAACTAC
  GACCTTGTCTACCTTCACTGCGAGGTGTACCTATGTGACTCTACGAGTGAACAGTGTAAACCTACCTGCT
  CTGGTACTAGATTTCGAAGTGGGAACTTCATAGATCAGACCCGTGTCCTGAACTTGGGTCCCATAACACG
  ACAAGGTGTCCAGGCCTCAGTGTCCAAGGCTGCTTCCAGCAACTTGAGGCTCCTGAGCATCTGGCTGCTG
  TTGTTTCCCTCAGCCACTTTGATCTTCATGGTTCAATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001278605
• Insert Size: 1929 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001278605.1, NP_001265534.1
• RefSeq Size: 2581 bp
• RefSeq ORF: 1929 bp
• Locus ID: 22242
• UniProt ID: Q91X17
• Cytogenetics: 7 63.88 cM
• Gene Summary: This gene encodes a glycoprotein that is the most abundant protein in mammalian urine under physiological conditions. It is synthesized in the kidney as a glycosyl-phosphatidylinositol anchored protein and released into urine as a soluble form by proteolytic cleavage. It is thought to regulate water and salt balance in the thick ascending limb of Henle and to protect against urinary tract infection and calcium oxalate crystal formation. In mouse deficiency of this gene is associated with increased susceptibility to bacterial infections and formation of calcium crystals in kidneys. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same protein.