Rnf112 (NM_001291024) Mouse Untagged Clone

CAT#: MC228529

Rnf112 (untagged) - Mouse ring finger protein 112 (Rnf112), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rnf112
• Synonyms: bfp; neurolastin; Zfp179; ZNF179
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228529 representing NM_001291024
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGAGGCCCGTCCTGTCAGTCACTGCTTTTTGTCATCGGCTTGGCAAACGGGAGAGCAAACGAAGCT
  TCATGGGAAACAGCAGCAACAGTTGGTCCCATGCATCATTCCCCAAGCTGGAGCTGGGCCTGGGACACCG
  TCCCTCCCCAACCCGGGAGCCGCCCACCTGCTCCATCTGTCTGGAAAGGCTTCGAGAGCCCATCTCGCTG
  GACTGTGGCCATGACTTCTGCATCCGATGCTTCAGCACACACCGCATCCCAGGCTGTGAGCTACCATGCT
  GTCCTGAATGCCGGAAGATCTGTAAGCAAAGGAAGGGCCTCCGCAGTCTAGGGGAAAGGATGAAACTCCT
  ACCACAGCGGCCACTGCCCCCTGCACTGCAGGAGACCTGTGCTGTGAGGGCAGAACGTCTGCTGTTGGTA
  CGAATCAATGCCTCCGGAGGCCTCATCCTCAGGATGGGCGCCATAAACCGTTGCCTGAAGCATCCACTGG
  CCAGGGACACACCCGTCTGCTTATTGGCTGTCCTGGGAGAGCAGCACTCAGGAAAGTCCTTCCTTTTGGA
  CCACTTGCTCAGTGGCTTACCAAGCCTGGAATCTGGTGACAGCGGCAGGCCCAGAGCAGAGGGGTCTCTG
  CCTGGGATCAGATGGGGCGCTAACGGCCTCACGAGGGGTATCTGGATGTGGAGTCACCCCTTCCTGCTGG
  GAAAAGAGGGGAAGAAGGTGGCTGTGTTCTTAGTGGACACGGGGGATGTCATGAGCCCAGAACTGAGCAA
  GGAGACAAGGGTCAAGCTCTGTGCTCTCACCATGATGCTCAGTTCCTACCAGATCCTGAATACCTCCCAA
  GAGCTGAAGGATACCGATCTGGGCTATCTAGAGATGTTCGTTCACGTGGCTGAGGTGATGGGCAAACATT
  ATGGGATGGTACCAATCCAGCATCTGGATCTCTTAGTCCGAGACTCTTCCCATCATAATAAGTCAGGGCA
  AGGGCACGTGGGTGACATACTCCAGAAGCTGTCCGGCAAATACCCCAAGGTCCAGGAGCTGCTCCTCGGG
  AAACGGGCCCGCTGTTACCTCCTCCCTGCTCCTGAGAGGCAGTGGGTAAACAAAGACCAAGCCAGCCCCA
  GAGGCAACACAGAAGATGACTTCTCCCACCATTTCCGGGCCTACATCTTGGACGTGCTGAGCACAGCCCC
  TCAGCACGCTAAGAGCCGCTGTCAAGGGTACTGGAGTGAGGGGCGTGCCGTGGCCAGGGGAGACAGACGC
  CTGCTCACAGGGCAGCAGCTAGCACAGGAGATCAAGAATCTCTCTGGCTGGATGGGGAAGACTGGGCCCA
  GTTTCAACTCTCCTGATGAGATGGCTGCTCAGCTCCATGATCTGAGGAAAGTGGAAGCCGCCAAGAAGGA
  GTTCGAGGAGTACGTGAGACAGCAGGACATAGCCACCAAGCGCATCTTCTCTGCACTACGAGTCCTGCCT
  GACACGATGAGGAACCTCCTCTCTACCCAGAAGGATGCCATCTTGGCCCGCCATGGTGTGGCCCTGTTGT
  GCAAGGAGAGAGAGCAGACCTTGGAGGCCCTGGAAGCCGAGCTGCAGGCAGAAGCCAAGGCCTTCATGGA
  CTCCTACACAATGCGCTTCTGTGGCCACCTGGCCGCGGTAGGGGGCGCTGTAGGTGCTGGGCTCATGGGC
  CTGGCAGGGGGTGTGGTGGGCGCAGGCATGGCAGCAGCGGCGTTGGCTGCTGAGGCTGGGATGGTAGCAG
  CTGGGGCCGCGGTGGGGGCCACCGGGGCTGCTGTGGTTGGGGGTGGTGTGGGTGCTGGTCTGGCCGCCAC
  TGTGGGCTGCATGGAGAAAGAGGAAGATGAGAGAGTTCAAGGAGGGGACCGAGAGCCCCTACTCCAGGAG
  GAATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001291024
• Insert Size: 1896 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001291024.1, NP_001277953.1
• RefSeq Size: 3029 bp
• RefSeq ORF: 1896 bp
• Locus ID: 22671
• UniProt ID: Q96DY5
• Cytogenetics: 11 37.96 cM
• Gene Summary: E3 ubiquitin-protein ligase that plays an important role in neuronal differentiation, including neurogenesis and gliogenesis, during brain development. During embryonic development initiates neuronal differentiation by inducing cell cycle arrest at the G0/G1 phase through up-regulation of cell-cycle regulatory proteins (PubMed:21566658, PubMed:28684796). Plays a role not only in the fetal period during the development of the nervous system, but also in the adult brain, where it is involved in the maintenance of neural functions and protection of the nervous tissue cells from oxidative stress-induced damage (PubMed:27918959, PubMed:26792191, PubMed:26951452). Exhibits GTPase and E3 ubiquitin-protein ligase activities. Regulates dendritic spine density and synaptic neurotransmission; its ability to hydrolyze GTP is involved in the maintenance of dendritic spine density (PubMed:26212327).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an alternate in-frame exon and uses alternate in-frame splice sites in the 5' coding region, compared to variant 3. It encodes isoform 2, which lacks two separate internal segments and is shorter, compared to isoform 3.