Atg9a (NM_001288613) Mouse Untagged Clone

CAT#: MC228500

Atg9a (untagged) - Mouse autophagy related 9A (Atg9a), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Atg9a
• Synonyms: Apg9l1; Atg9; Atg9l1; AU019532
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228500 representing NM_001288613
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGGCCCTGGTGAACAAATCCCTCCTGCCTCTGCGCTTCCGTCTGCCCGGCCTCGGAGAGGTTGTCT
  TCTTCACCCGTGGCCTCAAGTACAACTTTGAGCTCATCCTCTTCTGGGGACCCGGCTCTCTGTTTCTCAA
  TGAGTGGAGCCTCAAGGCCGAGTACAAACGTGGAGGGCAACGGCTAGAGCTGGCCCAGCGTCTCAGCAAC
  CGCATCTTGTGGATTGGCATCGCCAACTTCCTGCTGTGTCCCCTCATCCTCATCTGGCAGATCCTCTATG
  CCTTCTTCAGCTATGCCGAGGTGCTGAAGAGAGAGCCGGGGGCCCTGGGAGCGCGTTGCTGGTCACTCTA
  TGGCCGTTGTTACCTCCGCCACTTCAATGAGCTGGAGCATGAGCTGCAGTCCCGCCTCAACCGAGGCTAC
  AAGCCCGCCTCCAAGTACATGAATTGCTTCTTGTCACCGCTGCTGACTCTGCTGGCCAAGAATGGTGCCT
  TCTTCGCTGGCTCTATCCTGGCTGTGCTTATTGCCCTCACCATCTATGATGAAGATGTGTTAGCTGTGGA
  ACACGTCCTCACCACGGTCACCCTCCTGGGAGTCACGGTGACTGTGTGCAGGTCCTTCATCCCAGACCAG
  CACATGGTGTTCTGCCCCGAGCAGCTGCTCCGAGTGATTCTTGCACACATCCACTACATGCCTGACCACT
  GGCAGGGTAATGCCCACCGCTCGCAGACCCGGGACGAGTTTGCCCAGCTCTTCCAGTACAAGGCAGTGTT
  CATCTTGGAGGAGTTGCTGAGTCCCATCGTCACACCCCTCATTCTCATCTTCTGCCTCCGCCCTCGGGCC
  CTGGAGATCATAGACTTCTTCCGCAACTTTACGGTCGAGGTCGTGGGTGTGGGAGACACCTGCTCCTTTG
  CTCAGATGGACGTTCGCCAGCATGGCCATCCTCAGTGGCTGTCTGGAGGGCAGACAGAGGCCTCAGTGTA
  CCAGCAAGCCGAGGACGGGAAGACCGAGTTGTCGCTCATGCACTTTGCCATCACCAATCCCGGCTGGCAG
  CCCCCTCGTGAGAGCACAGCTTTCCTGGGCTTCCTCAAGGAGCAGGTGCAGCGAGATGGAGCAGCTGCTG
  GCCTGGCCCAGGGTGGCCTGCTCCCCGAGAATGCCCTCTTCACATCCATCCAGTCCTTACAGTCTGAGTC
  CGAGCCACTGAGCCTTATTGCAAATGTGGTAGCAGGCTCATCCTGCCGAGGACCCTCACTGTCCAGAGAC
  CTGCAGGGCTCCAGGCACAGGGCTGATGTTGCTTCTGCCCTTCGATCCTTCTCCCCTCTGCAGCCTGGAG
  CGGCCCCTCAAGGCCGGGTTCCCAGTACCATGACAGGCTCTGGAGTGGATGCCAGGACAGCCAGCTCTGG
  GAGTAGCGTGTGGGAAGGACAGCTGCAGAGCCTGGTGCTGTCCGAATACGCGTCCACCGAGATGAGCCTG
  CACGCCCTGTACATGCACCAGCTCCACAAGCAGCAGACCCAGGCTGAGCCCGAGCGGCATGTGTGGCACC
  GCCGGGAGAGTGATGAGAGTGGAGAGAGTGCCCCTGAAGAGGGGGGAGAGGGTGCCCGGGCCCCCCAACC
  CATCCCCCGCTCGGCCAGCTATCCCTGTGCTACACCCCGGCCTGGAGCACCTGAGACCACCGCCCTGCAT
  GGGGGCTTCCAGAGGCGCTACGGGGGCATCACAGATCCTGGCACAGTGCCCCGTGGCCCCTCTCACTTCT
  CCAGGCTGCCCCTGGGAGGATGGGCAGAAGATGGCCAGCCAGCATCAAGGCACCCAGAGCCGGTGCCAGA
  GGAGGGCTCAGAGGATGAACTCCCCCCTCAAGTGCACAAGGTATAG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_001288613
• Insert Size: 1866 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001288613.1, NP_001275542.1
• RefSeq Size: 4104 bp
• RefSeq ORF: 1866 bp
• Locus ID: 245860
• Cytogenetics: 1 C4
• Gene Summary: Involved in autophagy and cytoplasm to vacuole transport (Cvt) vesicle formation. Plays a key role in the organization of the preautophagosomal structure/phagophore assembly site (PAS), the nucleating site for formation of the sequestering vesicle. Cycles between a juxta-nuclear trans-Golgi network compartment and late endosomes. Nutrient starvation induces accumulation on autophagosomes. Starvation-dependent trafficking requires ULK1, ATG13 and SUPT20H (By similarity). Required for carbonyl cyanide m-chlorophenylhydrazone (CCCP)-induced ATG8 family proteins lipidation, a key autophagy step.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) uses an alternate splice junction at the 5' end of an exon and initiates translation at an alternate start codon, compared to variant 1. The resulting isoform (b) is shorter at the N-terminus compared to isoform a.