Ssx2ip (NM_001253770) Mouse Untagged Clone

CAT#: MC228474

Ssx2ip (untagged) - Mouse synovial sarcoma, X breakpoint 2 interacting protein (Ssx2ip), transcript variant 4

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ssx2ip
• Synonyms: Adip; AU014939; AU042321
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228474 representing NM_001253770
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAGATTGGATGACTGTGACAGATCCAGTTCTGTGTACAGAAAACAAAAATCTCTCTCAATATACCT
  CAGAAACAAAGATGTCTCCGTCCAGTTTGTACTCCCAGCAAGTTCTGTGCTCTTCAGTACCTTTATCCAA
  AAACGTGCATGGTGTTTTCGGTGTCTTCTGCACAGGAGAGAACATTGAACAAAGTATTTCCTATCTTGAT
  CAGGAGCTGACCACCTTCGGGTTTCCTTCCTTGTATGAAGAATCCAAAAGTAAAGAGGCAAAGAGAGAAT
  TAAATATAGTCGCTGTTCTGAACTGTATGAACGAGCTGCTCGTGCTTCAGCGGAAGAACCTGCTGGCCCA
  GGAGAGCGTGGAGACACAGAACTTGAAGCTGGGCAGTGACATGGACCACCTGCAGAGCTGCTACGCCAAA
  CTTAAGGAGCAGTTGGAAACGTCCAGGCGGGAGATGATCGGGCTTCAAGAGAGAGACAGGCAGCTGCAGT
  GCAAGAACAGGAGTTTGCATCAGCTCCTGAAGAATGAGAAAGATGAGGTACAAAAATTACAAAATATCAT
  AGCCAGCCGGGCTACTCAGTATAATCATGATGTGAAGAGGAAGGAGCGTGAATATAATAAGCTAAAGGAG
  CGCCTGCATCAGCTCGTTATGAACAAGAAGGATAAAAACATAGCCATGGATGTTTTAAATTATGTGGGTC
  GAGCTGATGGCAAACGAGGCTCATGGAGGACTGACAAAACAGAAGCCAGGAATGAAGATGAGATGTACAA
  AATTCTGTTGAATGATTATGAGTACCGCCAGAAGCAGATCCTGATGGAGAACGCGGAGCTGAAGAAGGTC
  CTCCAGCAGATGAAGAAGGAGATGATCTCTCTCCTGTCTCCTCAGAAGAAGAAGCCCAGGGAAAGAGCAG
  AGGACGGCACAGGCACTGTTGCTATCTCCGATATAGAAGATGACTCTGGGGAACTGAGCAGAGACAGCGT
  GTGGGGCCTTTCCTGTGACACTGTGAGAGAGCAGCTGACAAACAGCATCAGGAAACAGTGGAGAATTTTG
  AAAAGTCATGTAGAAAAACTCGATAACCAAGCTTCGAAGGTACACTCAGAGGGCCTTAATGAGGAGGACG
  TCATCTCACGACAAGACCATGAGCAAGAGACTGAGAAACTGGAGCTGGAGATTGAGCGGTGTAAAGAGAT
  GATCAAGGCTCAGCAGCAGCTCTTACAGCAGCTGGCCACCACGTGTGATGATGACACCACCTCACTGTTG
  CGAGACTGTTACTTGCTGGAAGAAAAGGAACGCCTTAAAGAAGAGTGGACCCTTTTTAAAGAGCAAAAAA
  AGAATTTTGAGAGAGAAAGGCGAAGCTTTACAGAAGCTGCCATTCGATTGGGGTTGGAGAGAAAGGCGTT
  TGAAGAAGAGCGAGCCAGCTGGGTAAAGCAGCAGTTTTTAAACATGACGAACTTTGACCACCAGAACTCA
  GAAAATGTGAAACTTTTCAGTGCCTTCTCAGGAAGTTCTGATCCAGACAATCTTATAGTCCACTCACGGC
  CACGGCAAAAGAAGCTACACAGTGTGGCTAATGGGGTGCCAGCTTGCACATCAAAACTGACTAAATCTCT
  TCCTGCCTCACCTTCTACTTCAGACTTTCGCCAGACACATTCATGTGTGTCTGAACACAGTTCCATCAGT
  GTGCTGAATATAACTCCTGAAGAAAGTAAACCAAGTGAGGTTGCAAGAGAAAGCACGGATCAGAAGTGGA
  GCGTGCAGTCGAGGCCCAGCTCGCGGGAGGGGTGCTACAGCGGATGCTCCTCGGCCTTCAGGAGCGCTCA
  CGGGGACCGAGATGACTTACCTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001253770
• Insert Size: 1845 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001253770.1, NP_001240699.1
• RefSeq Size: 3451 bp
• RefSeq ORF: 1845 bp
• Locus ID: 99167
• UniProt ID: Q8VC66
• Cytogenetics: 3 H2
• Gene Summary: Belongs to an adhesion system, which plays a role in the organization of homotypic, interneuronal and heterotypic cell-cell adherens junctions (AJs). May connect the nectin-afadin and E-cadherin-catenin system through alpha-actinin and may be involved in organization of the actin cytoskeleton at AJs through afadin and alpha-actinin (PubMed:12446711). Acts as a centrosome maturation factor, probably by maintaining the integrity of the pericentriolar material and proper microtubule nucleation at mitotic spindle poles. The function seems to implicate at least in part WRAP73; the SSX2IP:WRAP73 complex is proposed to act as regulator of spindle anchoring at the mitotic centrosome (By similarity). Involved in cell movement: localizes at the leading edge of moving cells in response to PDGF and is required for the formation of the leading edge and the promotion of cell movement, possibly via activation of Rac signaling (PubMed:22027834). Involved in ciliogenesis (By similarity). It is required for targeted recruitment of the BBSome, CEP290, RAB8, and SSTR3 to the cilia (By similarity).[UniProtKB/Swiss-Prot Function]